Relative abundance of proteins identified in MALDI IMS

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binding sites 58 of the 473 unambiguously assigned phosphorylation sites are predicted by Scansite to be sites for binding. 50 of these correspond.
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Correlation of log-transformed signal intensity from two Affymetrix microarray hybridizations using platelet RNA. Plotted are those probesets with an average.
Sequence alignment of C-terminal phosphorylated plant aquaporins
Enrichment of sequence disorder in the cytosolic phosphoproteome.
Phosphorylation and sequence disorder in microtubule-associated protein Tau.A, schematic illustration of the domain profile of Tau with all known phosphorylation.
NSAF and GeneChip datasets have a similar variance-versus-mean dependence.A–C, the rowMean and the rowS.D. of the abundance values for each transcript.
Phosphopeptides identified harboring minimal binding motifs
Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.
Novel phosphorylation sites on H+-ATPase proteins
Plasma CEP adducts and autoantibodies by donor age.
A, high resolution MS/MS spectrum (lower panel) of 1435
Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,
Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA. A, relative quantitation over three.
Top-down protein identification.
Two-dimensional electrophoresis results and validation with Western blotting. Two-dimensional electrophoresis results and validation with Western blotting.
Significantly enriched phosphorylation motifs from up-regulated phosphopeptides by Motif-X analysis. Significantly enriched phosphorylation motifs from.
Frequency distribution of the effect size measure (ESM) of sperm protein spots of type-1 diabetic (A), type-2 diabetic (B) and non-diabetic obese (C) patients.
Novel p53 target genes identified by RNA-Seq, pSILAC and ChIP-Seq.
Assay of NOS activity. Assay of NOS activity. Box show total NOS activity, the calcium-dependent activity of the constitutive isoforms of NOS (eNOS and.
Bottom-up proteomic characterization of MALDI IMS samples.
Representative example of LAXIC performance for complex plant phosphoproteome. Representative example of LAXIC performance for complex plant phosphoproteome.A.
Success rates in validation of antibodies from external providers
Results from the Morris water task.
Degree of glycosylation of human milk LF from individual donors across lactation. Degree of glycosylation of human milk LF from individual donors across.
NanoLC-MS/MS/based analysis of proteome differences between colonospheres and isogenic differentiated tumor cells. NanoLC-MS/MS/based analysis of proteome.
The evolutionary conservation of the phosphoproteomes.a, E. coli. b, B. subtilis. The evolutionary conservation of the phosphoproteomes.a, E. coli. b,
Cluster analysis and pathway-based characterization of differentially expressed genes and proteins from integrated proteomics. Cluster analysis and pathway-based.
Colonopshere-enriched proteins display functional interactions.
A, schematic presentation of fetuin-A domains.
Proteins previously reported in published MALDI IMS studies and their frequency of observation in the present study. Proteins previously reported in published.
Homologs between yeast and human seven-β-strand methyltransferases.
Molecular network analysis of up-regulated genes and proteins in NF1-KD PC12 cells. Molecular network analysis of up-regulated genes and proteins in NF1-KD.
Schematic summarizing the various functions and features of MASH Suite Pro. Schematic summarizing the various functions and features of MASH Suite Pro.
Tissue Western analysis of purified monospecific antibodies.
Two-dimensional gel electrophoresis of CyDye-labeled human sperm proteomes (DIGE) and identification of diabetes- and obesity-associated sperm proteins.
Testing the effectiveness of the three-step peptide fractionation method.A, μLC mass chromatograms of SCX fractions for an acidic FFE fraction. Testing.
Altered pathways in prostate cancer.
Interaction networks of the regulated phosphoproteins.
The PPAR-α agonist GW7647 increases the levels of apoA-I in the retina and the fibrous sclera. The PPAR-α agonist GW7647 increases the levels of apoA-I.
IEF 2D PAGE of whole protein extracts from breast apocrine macrocysts
Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum. Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum.A,
Plot of the deviation of the predicted pI value of every peptide spectrum from the average pI calculated for each fraction for validated (a) and non-validated.
Relative quantification of cis and trans PSP gp10040–42/47–52 variants
Distribution of the phosphoproteins based on GO analysis, including biological process (Left) and cellular component (Right). Distribution of the phosphoproteins.
Extraction of proteins from MALDI IMS slides.
Differential expression of apoA-I and Vimentin on 2D gels
Significant alterations in cysteine and methionine metabolism.
K-Means clustering of protein and mRNA expression patterns after PPAR agonists treatments. k-Means clustering of protein and mRNA expression patterns after.
Number of genes/antibodies included in the database.
Immuno-MS results from antibodies toward 20 different target proteins in HeLa cell lysates. Immuno-MS results from antibodies toward 20 different target.
Bar plot representation of the transcriptomic changes in Δsaci_ptp and Δsaci_pp2a. Bar plot representation of the transcriptomic changes in Δsaci_ptp and.
Localization of selected clones in mammalian COS-7 cells.
Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. Analytical.
A and B, ROC curves of the proteomics panel diagnosis.
Biochemical characterization of the protein phosphatases Saci-PTP.
Changes in mRNA levels do not correlate with changes in protein levels in upf1Δ and xrn1Δ cells. Changes in mRNA levels do not correlate with changes in.
Illustration of chromatography metric C-2A applied to LC-MS/MS data from three Thermo LTQ systems in analyses of yeast proteome samples in CPTAC Study.
Classification of the 1458 identified proteins into molecular functions. Classification of the 1458 identified proteins into molecular functions. The pie.
Separation of colonospheres from differentiated tumor cells by cluster analysis. Separation of colonospheres from differentiated tumor cells by cluster.
Comparison of HCT-8 cell line proteome under apoptotic conditions in response to 10 mm Gln treatment Spot numbers with corresponding Swiss-Prot database.
2-D gel images visualized by Coomassie Brilliant Blue staining representing total proteins extracted from HCT-8 under apoptotic conditions in 2 mm Gln.
Expression of σ in SCCs Expression of σ in SCCs. Shown is a magnified section of a representative 2D PAGE gel run with a lysate from an SCC.
Antibody specificity ascertained by 2D-PAGE Western immunoblotting (IEF) of total cellular protein extracts from the RT4 human bladder cancer cell line.
Schematic of AIMS-to-MRM experiment.
GeneGoTM-based signaling pathway annotations of proteins identified in CD56+ NK cell subsets. GeneGoTM-based signaling pathway annotations of proteins.
Changes in protein expression during distinct stages of NK cell differentiation. Changes in protein expression during distinct stages of NK cell differentiation.
The average median S.D. and PEV reduction after applying different normalization methods compared with raw data. The average median S.D. and PEV reduction.
Peptide mass spectra of SUMO target proteins.
Phosphopeptides identified harboring minimal binding motifs
Model of the change in receptor structure on engagement of the ligand IFN-γ. Model of the change in receptor structure on engagement of the ligand IFN-γ.
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Relative abundance of proteins identified in MALDI IMS Relative abundance of proteins identified in MALDI IMS. A, Proteins from all 10 human tissues were ranked according to the number of assigned spectra divided by the molecular weight of the proteins' database entry. Relative abundance of proteins identified in MALDI IMS.A, Proteins from all 10 human tissues were ranked according to the number of assigned spectra divided by the molecular weight of the proteins' database entry. MALDI IMS markers extracted from the literature (see also Table I and supplemental Table S5) are indicated in orange showing that these are mainly abundant cellular proteins. B, Proteins identified from the MALDI matrix layer plotted against the ranked protein abundance used in panel A). Stefan K. Maier et al. Mol Cell Proteomics 2013;12:2901-2910 © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.