M. Ylihärsilä, E. Harju, R. Arppe, L. Hattara, J. Hölsä, P

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Genotyping of clinically relevant human adenoviruses by array-in-well hybridization assay M. Ylihärsilä, E. Harju, R. Arppe, L. Hattara, J. Hölsä, P. Saviranta, T. Soukka, M. Waris Clinical Microbiology and Infection Volume 19, Issue 6, Pages 551-557 (June 2013) DOI: 10.1111/j.1469-0691.2012.03926.x Copyright © 2013 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 1 Distribution of real-time PCR cycle threshold (Ct) values for the human adenovirus (hAdV)-typed specimens and unspecific amplification of hAdV-negative specimens or specimens with other viruses. DNA from prototypes (stars) was extracted from culture supernatants with varying level of infection and their Ct values do not reflect the assay sensitivity. Clinical Microbiology and Infection 2013 19, 551-557DOI: (10.1111/j.1469-0691.2012.03926.x) Copyright © 2013 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 2 The array-in-well layout. The symbols C1, C2 and C3 denote generic control probe spots and spot numbers 1–16 correspond to the specific probes listed in the Supplementary material, Data S3. The probe number does not refer to the human adenovirus (hAdV) genotype. (b) Hybridization patterns of representative prototypes from hAdV genotypes C01, C02, B03, E04, C05, C06, B07, B11, B14, D19, B21, A31, D36, D37, clinical specimens D08 and F41 (genotypes were determined by sequencing), non-targeted hAdV prototype A18, and hAdV-negative specimen. The assignment to hAdV type in unknown specimens was based on the hybridization pattern in comparison to that of the known types. Genotype C02 dilution 1 (dil.1): 5 × 105 copies, dil.2: 500 copies, and dil.3: 5 copies per PCR, respectively. Clinical Microbiology and Infection 2013 19, 551-557DOI: (10.1111/j.1469-0691.2012.03926.x) Copyright © 2013 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 3 Adenovirus genotypes detected in different age groups. Sporadic types in the age group 0–4 years: E04 and F41; 5–17 years: C01, C05, D19 and D37; 18–67 years: D08 and A31. Clinical Microbiology and Infection 2013 19, 551-557DOI: (10.1111/j.1469-0691.2012.03926.x) Copyright © 2013 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 4 Adenovirus genotypes detected in different specimen types (n = 216). NP = nasopharyngeal aspirate or swab and other respiratory specimens. Others include tissue, urine and colonoscopy specimens. Sporadic types in ocular swab specimens: D08; in stool specimens: C01, E04, A31 and F41; in unknown specimens: C02 and B03. Clinical Microbiology and Infection 2013 19, 551-557DOI: (10.1111/j.1469-0691.2012.03926.x) Copyright © 2013 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 5 Monthly distribution of detected adenovirus genotypes. The number of samples is presented in relation to the total number of specimens (positive and negative) analysed by PCR, multiplex PCR, antigen detection and virus culture during these months. Clinical Microbiology and Infection 2013 19, 551-557DOI: (10.1111/j.1469-0691.2012.03926.x) Copyright © 2013 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions