Titration of Abundance for Purified GST-Nups

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Presentation transcript:

Titration of Abundance for Purified GST-Nups 7 50 mL GST-Nup 6 5 mL GST-Nup 8 1mg BSA 9 2mg BSA 10 4mg BSA 3 +IPTG 2 -IPTG 5 blank 4 MSS 1 MW 7 50 mL GST-Nup 6 5 mL GST-Nup 8 1mg BSA 9 2mg BSA 10 4mg BSA 3 +IPTG 2 -IPTG 5 blank 4 MSS 1 MW 7 50 mL GST-Nup 6 5 mL GST-Nup 8 1mg BSA 9 2mg BSA 10 4mg BSA 3 +IPTG 2 -IPTG 5 blank 4 MSS 1 MW A GST-Nsp1h (1-172) B GST-Nup116h (165-715) C GST-Nup100h (1-640) D GST-Nup145h (1-242) E GST-Nup1h (798-1076) F GST-Nsp1 (377-471) K>R G GST-Nsp1 (377-471) wild type H GST-Nup42 (1-382)

Titration of Abundance for Purified GST-Nups Group GST-Nup Amino Acids Molecular Weight (daltons) A GST-Nsp1h (1-172) 1-172 46,653 B GST-Nup116h (165-715) 165-715 83,844 C GST-Nup100h (1-640) 1-640 93,835 D GST-Nup145h (1-242) 1-242 53,498 E GST-Nup1h (798-1076) 798-1076 57,092 F GST-Nsp1 (377-471) K>R 371-471 39,841 G GST-Nsp1 (377-471) wild type 377-471 39,365 H GST-Nup42 (1-382) 1-382 67,144