run_pepstats.pl parse_pepstats.pl parse_pepstats.pl.

Slides:



Advertisements
Similar presentations
Separation and Purification of Macromolecules. Why? Molecules each have specific features: Mass Charge Hyrdopathicity.
Advertisements

The construction of cells DNA or RNA Protein Carbohydrates Lipid etc.
The construction of cells DNA or RNA Protein Carbohydrates Lipid etc. 04.
The construction of cells DNA or RNA Protein Carbohydrates Lipid etc
Prokaryote vs. Eukaryote
The construction of cells DNA or RNA Protein Carbohydrates Lipid etc
The construction of cells DNA or RNA Protein Carbohydrates Lipid etc.
How does it actually work?.  Lysis buffers are typically detergents such as sodium dodecyl sulfate (SDS)  What happens if you add dish soap to a frying.
Your Initials Your Initials
The Cell and Cell Structures How are cells structured to do the “right” thing?
Cells Section 1 – Cell Structure A. Common cell structures – outer covering called cell membrane and internal gelatin like cytoplasm. A. Comparing cells.
Lab 6 Salt fractionation In aqueous environments salt ions interact strongly with their complementary charge on a water molecule If the concentration of.
The Cell is like a Factory
Key points for Bacteria. Archaebacteria vs. Eubacteria Both have no nucleus or membrane bound organelles. No peptidoglycan at all in cell wall Several.
The differences between plant and animal cells What are the differences between them? Animal cells Plant cells.
Go Bananas! Introduction Tell you about DNA Show you how to extract DNA from a Banana.
Cell Structure and Function
Bacterial Transformation
Objective: What are the functions of the different organelles within cells?
Have your clickers ready, and get out a piece of paper to record your answers on in case of a technological blip.
Free Response ? Unit 2 - Cells Question # AP Exam.
DNA and Protein Synthesis Ch. 12 and 13. Youtube Review
MCB 3421 computer lab 14. student evaluations Please follow this link to the on-line surveys that are open for you this semester. If you are done, check.
 DNA is found in the cell of every living thing.  Proteins that help DNA maintain it’s form may obscure our ability to see the DNA so they must be removed.
A Cell Review Game By: Ms. Huber RIDDLE ME THIS….
Lab 6 Ig Purification What will the Ig be used for? How pure does it need to be? What is the source of the antibody (serum, ascites, cell culture supernatant)
Exit Questions Sept. 11, What is the function of the Chloroplast? 2. What is the function of the Cell Wall? 3. What is the function of a vacuole?
Amine R group Alpha Carbon Carboxylic Acid. Nonpolar side chains.
Tutorial 11 Modes and Methods in Capillary Electrophoresis
Day Date Subject To be read prior to this class period:
Compare and contrast two cell groups
1. What is the pH range of acids?
Ion Exchange Chromatography
DNA Extraction and Isolation
Cell Structure & Function
9 Square Vocabulary Builder
Prokaryotic and Eukaryotic
Bacteria Dagan kirschner.
No membrane bound nucleus Only distinct organelle ribosome
Pensacola High School IB Chemistry 3
Student evaluations Please go to husky CT and complete student evaluations !
Cell Theory and Prokaryote vs. Eukaryote
What is a cell? Basic functioning unit in unicellular and multicellular organisms.
محاضرة عامة التقنيات الحيوية (هندسة الجينات .. مبادئ وتطبيقات)
Cell Membrane protective layer around all cells
Is DNA in your food or just in you?
PGLO Lab Purpose: To transform E. coli bacteria by adding plasmids that allow the bacteria to glow green under UV light in the presence of arabinose sugar.
19.2 Amino Acids as Acids and Bases
KNOWING UNDERSTANDING.
P. 792.
DNA Extraction Lab.
Membrane and Transport Notes
Cells.
Gel Electrophoresis.
DNA and Protein Synthesis
CELL pp
Review from yesterday Write on a Post-It Note one fact about diffusion
Prokaryotes vs. Eukaryotes
THE CELL THEORY 1. All living things are composed of cells.
3.3 Cell Membrane.
Prokaryotic and Eukaryotic cells
Week 4 Vocab Definitions
Prokaryotic before nucleus Eukaryotic true nucleus.
Has membrane bound organelles Has no membrane bound organelles
All Cells Must Have……. Cell membranes Cytoplasm DNA Ribosomes
Properties of Water Liquid at most temperatures on earth
________________________ processes proteins makes membranes
Gel Electrophoresis.
Experiment 3 Separation of proteins by Anion exchange chromatography
Bacterial architecture and targets for biosensing.
Presentation transcript:

run_pepstats.pl

parse_pepstats.pl

parse_pepstats.pl

parse_pepstats_mod.pl

parse_pepstats_mod.pl

parse_pepstats_mod.pl

histogramScript_pdf.R

histogramScript_pdf.R

histogramScript_pdf.R

pH at which protein has no charge

DNA, cell wall & membrane bound proteins pH at which protein has no charge

pH at which protein has no charge

Histogram of theoretical isoelectric points of Thermplasmatales proteins pH at which protein has no charge

pH at which protein has no charge

Histogram of theoretical isoelectric points of Saccharomyces proteins DNA, cell wall & membrane bound proteins pH at which protein has no charge

Histogram of theoretical isoelectric points of Haloferax proteins Salt in strategy At neutral pH most proteins with negative charge pH at which protein has no charge