Volume 18, Issue 4, Pages (April 2011)

Slides:

Advertisements

Similar presentations

Development of Antibiotic Activity Profile Screening for the Classification and Discovery of Natural Product Antibiotics Weng Ruh Wong, Allen G. Oliver,

Advertisements

The Biosynthesis of Teicoplanin-Type Glycopeptide Antibiotics: Assignment of P450 Mono-Oxygenases to Side Chain Cyclizations of Glycopeptide A47934 Bianka.

DNA Topoisomerases and Their Poisoning by Anticancer and Antibacterial Drugs Yves Pommier, Elisabetta Leo, HongLiang Zhang, Christophe Marchand Chemistry.

Hoiamide A, a Sodium Channel Activator of Unusual Architecture from a Consortium of Two Papua New Guinea Cyanobacteria Alban Pereira, Zhengyu Cao, Thomas.

An Unnatural PIP Simulates Growth Factor Signaling

Volume 22, Issue 5, Pages v-vi (May 2015)

Enzyme Annotation with Chemical Tools

New Light on Methylthiolation Reactions

On-Bead Library Screening Made Easier

Antibiotics: A New Hope

Foundations for Directed Alkaloid Biosynthesis

Biosynthesis of the Antitumor Agent Chartreusin Involves the Oxidative Rearrangement of an Anthracyclic Polyketide Zhongli Xu, Kathrin Jakobi, Katrin.

Nature’s Strategy for Catalyzing Diels-Alder Reaction

Recent Developments in G-Quadruplex Probes

Chemical Proteomics of Host-Pathogen Interaction

Volume 15, Issue 2, Pages (February 2008)

Adding Specificity to Artificial Transcription Activators

Finding the Missing Code of RNA Recognition by PUF Proteins

Tuberculosis Terpene Targets

Volume 20, Issue 6, Pages (June 2013)

Volume 13, Issue 4, Pages (April 2006)

Huawei Chen, Sarah O’Connor, David E. Cane, Christopher T. Walsh

An FAD-Dependent Pyridine Nucleotide-Disulfide Oxidoreductase Is Involved in Disulfide Bond Formation in FK228 Anticancer Depsipeptide Cheng Wang, Shane.

Manipulation of Carrier Proteins in Antibiotic Biosynthesis

Polyketide Proofreading by an Acyltransferase-like Enzyme

Activity-Based Profiling of 2-Oxoglutarate Oxygenases

Targeting the Undruggable Proteome: The Small Molecules of My Dreams

Volume 19, Issue 2, Pages (February 2012)

A Revised Pathway Proposed for Staphylococcus aureus Wall Teichoic Acid Biosynthesis Based on In Vitro Reconstitution of the Intracellular Steps Stephanie.

A Noncanonical Path to Mechanism of Action

Volume 15, Issue 8, Pages (August 2008)

Redesign of a Dioxygenase in Morphine Biosynthesis

Volume 14, Issue 1, Pages (January 2007)

No Need To Be Pure: Mix the Cultures!

Volume 17, Issue 10, Pages (October 2010)

Elucidation of the Biosynthetic Gene Cluster and the Post-PKS Modification Mechanism for Fostriecin in Streptomyces pulveraceus Rixiang Kong, Xuejiao.

Carl J. Balibar, Sylvie Garneau-Tsodikova, Christopher T. Walsh

Volume 20, Issue 12, Pages (December 2013)

Insights into Bacterial 6-Methylsalicylic Acid Synthase and Its Engineering to Orsellinic Acid Synthase for Spirotetronate Generation Wei Ding, Chun.

Sherry S. Lamb, Tejal Patel, Kalinka P. Koteva, Gerard D. Wright

Volume 20, Issue 6, Pages (June 2013)

PqsE of Pseudomonas aeruginosa Acts as Pathway-Specific Thioesterase in the Biosynthesis of Alkylquinolone Signaling Molecules Steffen Lorenz Drees,

Macrophage Activation: On PAR with LPS

Volume 14, Issue 7, Pages (July 2007)

Evidence for a Protein-Protein Interaction Motif on an Acyl Carrier Protein Domain from a Modular Polyketide Synthase Kira J. Weissman, Hui Hong, Bojana.

Volume 21, Issue 8, Pages v-vi (August 2014)

An Artificial Pathway to 3,4-Dihydroxybenzoic Acid Allows Generation of New Aminocoumarin Antibiotic Recognized by Catechol Transporters of E. coli Silke.

Tandem Enzymatic Oxygenations in Biosynthesis of Epoxyquinone Pharmacophore of Manumycin-type Metabolites Zhe Rui, Moriah Sandy, Brian Jung, Wenjun Zhang

Foundations for Directed Alkaloid Biosynthesis

Volume 20, Issue 6, Pages (June 2013)

Volume 15, Issue 11, Pages (November 2008)

Characterization of the Biosynthetic Gene Cluster for Benzoxazole Antibiotics A33853 Reveals Unusual Assembly Logic Meinan Lv, Junfeng Zhao, Zixin Deng,

Gerald Lackner, Markus Bohnert, Jonas Wick, Dirk Hoffmeister

Nature’s Strategy for Catalyzing Diels-Alder Reaction

L-DOPA Ropes in tRNAPhe

Volume 12, Issue 6, Pages (June 2005)

Volume 16, Issue 4, Pages (April 2009)

Dual Carbamoylations on the Polyketide and Glycosyl Moiety by Asm21 Result in Extended Ansamitocin Biosynthesis Yan Li, Peiji Zhao, Qianjin Kang, Juan.

Volume 21, Issue 9, Pages (September 2014)

Volume 18, Issue 8, Pages (August 2011)

Volume 20, Issue 10, Pages (October 2013)

Interdomain Communication between the Thiolation and Thioesterase Domains of EntF Explored by Combinatorial Mutagenesis and Selection Zhe Zhou, Jonathan.

Tools to Tackle Protein Acetylation

Volume 14, Issue 9, Pages (September 2007)

Volume 22, Issue 11, Pages (November 2015)

A One-Pot Chemoenzymatic Synthesis for the Universal Precursor of Antidiabetes and Antiviral Bis-Indolylquinones Patrick Schneider, Monika Weber, Karen.

Volume 21, Issue 9, Pages (September 2014)

Volume 22, Issue 4, Pages vii-viii (April 2015)

Huawei Chen, Brian K Hubbard, Sarah E O'Connor, Christopher T Walsh

Polyketide Proofreading by an Acyltransferase-like Enzyme

Presentation transcript:

Volume 18, Issue 4, Pages 438-444 (April 2011) Supramolecular Templating in Kirromycin Biosynthesis: The Acyltransferase KirCII Loads Ethylmalonyl-CoA Extender onto a Specific ACP of the trans-AT PKS Ewa M. Musiol, Thomas Härtner, Andreas Kulik, Jana Moldenhauer, Jörn Piel, Wolfgang Wohlleben, Tilmann Weber Chemistry & Biology Volume 18, Issue 4, Pages 438-444 (April 2011) DOI: 10.1016/j.chembiol.2011.02.007 Copyright © 2011 Elsevier Ltd Terms and Conditions

Chemistry & Biology 2011 18, 438-444DOI: (10. 1016/j. chembiol. 2011 Copyright © 2011 Elsevier Ltd Terms and Conditions

Figure 1 Kirromycin Production Assay (A) HPLC/ESI-MS analysis of extracts of the wild-type S. collinus Tü 365. Left: HPLC UV/Vis trace. Right: mass spectrum of kirromycin (negative mode). (B) HPLC/ESI-MS analysis of extracts of the mutant ΔkirCII carrying the vector pRM4. (C) HPLC/ESI-MS analysis of extracts of the complemented mutant ΔkirCII pEM11CII. Left: HPLC UV/Vis trace. Right: mass spectrum of kirromycin (negative mode). See also Figures S1 and S2. Chemistry & Biology 2011 18, 438-444DOI: (10.1016/j.chembiol.2011.02.007) Copyright © 2011 Elsevier Ltd Terms and Conditions

Figure 2 HPLC/ESI-MS Analysis of the ACP4/ACP5-Loading Assay with Ethylmalonyl-CoA as Substrate for KirCII (A) ACP4. (B) ACP5. (I) Negative control containing ACP4/ACP5, pET52-proteins, no KirCII. (II) Loading assay containing ACP4/ACP5, ethylmalonyl-CoA, and KirCII. Left: HPLC UV/Vis trace. Central: mass spectrum. Right: deconvoluted spectrum generated with MagTran 1.03 (Zhang and Marshall, 1998). See also Figures S3, S6, and S7. Chemistry & Biology 2011 18, 438-444DOI: (10.1016/j.chembiol.2011.02.007) Copyright © 2011 Elsevier Ltd Terms and Conditions

Figure 3 Autoradiographic Analysis of the ACP4/ACP5-Loading Assay with [14C]malonyl-/[14C]methylmalonyl-CoA as Substrates for KirCII (A) Coomassie Brilliant Blue–stained gels and autoradiograms of the (I) ACP4- and (II) ACP5-loading assay with [14C]malonyl-CoA. Lane 1: positive control reaction for ACP4 containing apo-ACP4 and Sfp (detected signal is [14C]malonyl-holo-ACP4); lane 2: negative control for holo-ACP4 containing pET52-proteins, no KirCII; lane 3: holo-ACP4 and KirCII; lane 4: holo-ACP5 and KirCII; lane 5: positive control reaction for ACP5 containing apo-ACP5 and Sfp (detected signal is [14C]malonyl-holo-ACP5); lane 6: negative control for holo-ACP5 containing pET52-proteins, no KirCII. (B) Coomassie Brilliant Blue–stained gels and autoradiograms of the (I) ACP4- and (II) ACP5- loading assay with [14C]methylmalonyl-CoA. Lane 1: holo-ACP4 and KirCII; lane 2: positive control reaction for ACP4 containing apo-ACP4 and Sfp (detected signal is [14C]methylmalonyl-holo-ACP4); lane 3: negative control for holo-ACP4 containing pET52-proteins, no KirCII; lane 4: positive control reaction for ACP5 containing apo-ACP5 and Sfp (detected signal is [14C]methylmalonyl-holo-ACP5); lane 5: negative control for holo-ACP5 containing pET52-proteins, no KirCII; lane 6: holo-ACP5 and KirCII. See also Figure S3. Chemistry & Biology 2011 18, 438-444DOI: (10.1016/j.chembiol.2011.02.007) Copyright © 2011 Elsevier Ltd Terms and Conditions