TgCA_RP is most similar to the η-class carbonic anhydrase PfCA.

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TgCA_RP is most similar to the η-class carbonic anhydrase PfCA. TgCA_RP is most similar to the η-class carbonic anhydrase PfCA. (A) Sequence alignment of the amino acid sequence of TgCA_RP with several α-CAs (human carbonic anhydrase 1 [HuCA1], human carbonic anhydrase 2 [HuCA2], and Neisseria gonorrhoeae carbonic anhydrase [NgCA]) highlighting several distinct features. The canonical α-CA Zn2+ coordination site, which consists of three histidine (H) residues, is indicated with the symbols *, **, and ***. TgCA_RP shows two substitutions in this domain: a phenylalanine (F) replaces the first histidine (*) and a glutamine (Q) replaces the third conserved histidine (***). The two gateway amino acids, indicated with the symbols • and ••, glutamic acid and threonine, are conserved. Histidine64 (′′′), proposed to act as a proton shuttle in HuCA1, HuCa2, and NgCA, is replaced in TgCA_RP by a Lys (K). (B) Multiple sequence alignment of the TgCA_RP amino acid sequence with coccidian (Neospora caninum [NcCAh] and Eimeria tenella [EtCAh]) and piroplasm (Plasmodium berghei [PbCAh], Plasmodium yoelii [PyCAh], and Plasmodium falciparum [PfCA]) orthologs shows shared features. The proton shuttle histidine indicated with ′′′ is replaced by lysine (K) in the coccidian sequences (TgCA_RP, NcCA, and EtCA) and by asparagine (N) in the Aconoidasida sequences (PyCA, PbCA, and PfCA). The three histidine residues of the Zn2+ coordination domain are indicated with the symbols *, **, and ***. The first histidine is conserved in PfCA, but it is replaced by a phenylalanine (TgCA_RP1 and NcCAh), leucine (L) (EtCAh), or asparagine (PbCAh and PyCAh) in apicomplexan orthologs. The gatekeeper residues are indicated with the symbols • and ••, and both the glutamine and threonine are conserved among all members of this group. The orange lane indicates the GPI attachment consensus sequence. (C) Predicted structural alignment of the predicted TgCA_RP α-CA domain (red) with human carbonic anhydrase 2 (gray) showing the important active site residues referenced above in TgCA_RP, corresponding to the α-CA proton shuttle (Lys201, purple), gateway residues (Glu239 and Thr414, blue), and Zn2+ coordination residues (Phe233, His235, and Gln254, green). Nathan M. Chasen et al. mSphere 2017; doi:10.1128/mSphere.00027-17