Identification of a novel cosavirus species in faeces of children and its relationship with acute gastroenteritis in China  J.-M. Yu, Y.-Y. Ao, L.-L.

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Identification of a novel cosavirus species in faeces of children and its relationship with acute gastroenteritis in China  J.-M. Yu, Y.-Y. Ao, L.-L. Li, Z.-J. Duan  Clinical Microbiology and Infection  Volume 23, Issue 8, Pages 550-554 (August 2017) DOI: 10.1016/j.cmi.2017.02.018 Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 (a) Genome organization of Cosa-CHN. (b) Whole virus sequencing approach. (c) Protein cleavage map. Nine fragments were used to produce final genome sequence. Potential cleavage sites of Cosa-CHN are similar to those of HCosV B. Location of initial PCR for HCosV and Cosa-CHN specific reverse-transcription nested PCR amplicon products are indicated in red and marked as VP1 and VP3/VP1 respectively. Cosa-CHN, novel human cosavirus; HCosV, human cosavirus. Clinical Microbiology and Infection 2017 23, 550-554DOI: (10.1016/j.cmi.2017.02.018) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 2 Phylogenetic analysis of Cosa-CHN sequences. (a) Polyprotein amino acid sequences. (b) Partial VP3/VP1 nucleotide sequences. Tree was constructed using neighbour-joining method by MEGA5 software with 1000 bootstrap replicates. Sequences from this study are indicated by solid circles. Cosa-CHN formed lineage distinct from other known HCosV. Cosa-CHN, novel human cosavirus; HCosV, human cosavirus. Clinical Microbiology and Infection 2017 23, 550-554DOI: (10.1016/j.cmi.2017.02.018) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

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