Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA

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Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA Dwaine A Braasch, David R Corey Chemistry & Biology Volume 8, Issue 1, Pages 1-7 (January 2001) DOI: 10.1016/S1074-5521(00)00058-2

Fig. 1 Nucleic acid analogs studied for their potential as antisense agents. Phosphodiester DNA, phosphorothioate DNA, PNA, phosphoramidate DNA, morpholino and LNA. Chemistry & Biology 2001 8, 1-7DOI: (10.1016/S1074-5521(00)00058-2)

Fig. 2 LNAs bind tightly to DNA. Melting curves for an LNA oligonucleotide bound to complementary DNA compared with the melting curve for the analogous all-DNA duplex. The sequence of the LNA is 5′-AGGATCmeTAGG-3′ (Braasch, unpublished results). Chemistry & Biology 2001 8, 1-7DOI: (10.1016/S1074-5521(00)00058-2)

Fig. 3 Mechanisms for inhibition of gene. (A) Inhibition of gene expression by an antisense oligonucleotide that binds to the 5′-untranslated region and blocks binding of the translation apparatus. (B) Promotion of RNA cleavage and inhibition of gene expression by an antisense oligonucleotide that can activate RNase H. Chemistry & Biology 2001 8, 1-7DOI: (10.1016/S1074-5521(00)00058-2)

Fig. 4 Comparison of the properties of LNA and PNA. Chemistry & Biology 2001 8, 1-7DOI: (10.1016/S1074-5521(00)00058-2)