Variance in total levels of phospholipase C zeta (PLC-ζ) in human sperm may limit the applicability of quantitative immunofluorescent analysis as a diagnostic.

Slides:

Advertisements

Similar presentations

Egg Donor Informed Consent Tool (EDICT): development and validation of a new informed consent tool for oocyte donors Amanda Skillern, M.D., Marcelle.

Advertisements

Krinos M. Trokoudes, M. D. , Constantinos Pavlides, M. Sc

Direct vitamin D3 actions on rhesus macaque follicles in three-dimensional culture: assessment of follicle survival, growth, steroid, and antimüllerian.

Clinical outcome of non–hCG-primed oocyte in vitro maturation treatment in patients with polycystic ovaries and polycystic ovary syndrome Michel De Vos,

Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility Fabiola Zambrano, M.Sc., Tessa.

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolas Garrido, Ph

Edward G. Hughes, M.B., F.R.C.S.(C.), Deirdre DeJean, M.Sc.

Characterization of two heterozygous mutations of the oocyte activation factor phospholipase C zeta (PLCζ) from an infertile man by use of minisequencing.

Infertility evaluation and treatment among women in the United States

Ranjit S. Sandhu, B. S. , Timothy H. Wong, B. A. , Crystal A. Kling, B

Fernando Zegers-Hochschild, M.D. Fertility and Sterility

Nabil Aziz, M. D. , Ashok Agarwal, Ph. D. , HCLD, Iwan Lewis-Jones, M

Michael M. Alper, M.D. Fertility and Sterility

Urinary peptide profiling identifies a panel of putative biomarkers for diagnosing and staging endometriosis Muna M. El-Kasti, Ph.D., Cynthia Wright,

Sperm involved in recurrent partial hydatidiform moles cannot induce the normal pattern of calcium oscillations Dimitra Nikiforaki, M.Sc., Frauke Vanden.

Contrasting patterns in in vitro fertilization pregnancy rates among fresh autologous, fresh oocyte donor, and cryopreserved cycles with the use of day.

Reducing multiples: a mathematical formula that accurately predicts rates of singletons, twins, and higher-order multiples in women undergoing in vitro.

Phospholipase C zeta undergoes dynamic changes in its pattern of localization in sperm during capacitation and the acrosome reaction Claire Young, B.Sc.,

Azoospermia: is sample centrifugation indicated

Evaluation of the immature human oocytes from unstimulated cycles in polycystic ovary syndrome patients using a novel scoring system Shan Liu, M.D.,

Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis Pravin T. Goud, M.D., Ph.D., Anuradha P.

In vitro fertilization availability and utilization in the United States: a study of demographic, social, and economic factors Ahmad O. Hammoud, M.D.,

Live birth of twins derived from zona-free oocytes

Ali Sazci, Ph. D. , Nesrin Ercelen, M. D. , Emel Ergul, M. S

Double probing individual human spermatozoa: aniline blue staining for persistent histones and fluorescence in situ hybridization for aneuploidies Laszlo.

Feng Liu, B. Sc. , Yong Zhu, Ph. D

Diagnostic accuracy of sperm chromatin dispersion test to evaluate sperm deoxyribonucleic acid damage in men with unexplained infertility Cinthia M.

Multidrug-resistant transport activity protects oocytes from chemotherapeutic agents and changes during oocyte maturation Lynae M. Brayboy, M.D., Nathalie.

Koji Matsuura, Ph. D. , Takuya Uozumi, M. S. , Takuya Furuichi, Ph. D

Randomized, controlled pilot trial of natural versus hormone replacement therapy cycles in frozen embryo replacement in vitro fertilization Ginny Mounce,

Reproduction at an advanced maternal age and maternal health

Oocyte cryopreservation

Ramadan Saleh, M. D. , Reda Z. Mahfouz, M. D. , Ashok Agarwal, Ph. D

Genome-wide sperm deoxyribonucleic acid methylation is altered in some men with abnormal chromatin packaging or poor in vitro fertilization embryogenesis

Acetylated α-tubulin is reduced in individuals with poor sperm motility Shweta Bhagwat, M.Sc., Veena Dalvi, M.Sc., Darshan Chandrasekhar, M.Sc., Tinsu.

Reply of the Authors Fertility and Sterility

Gadolinium radiologic contrast is a useful alternative for hysterosalpingography in patients with iodine allergy Dorette Noorhasan, M.D., Michael J.

Looking back: egg donors' retrospective evaluations of their motivations, expectations, and experiences during their first donation cycle Nancy J. Kenney,

HOXA-10 and E-cadherin expression in the endometrium of women with recurrent implantation failure and recurrent miscarriage Yihua Yang, Ph.D., Xiaoyan.

Total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta are significantly correlated with fertilization rates after.

Limitations on the compensation of gamete donors: a public opinion survey Malinda S. Lee, M.D., M.B.A., Leslie V. Farland, Sc.D., Stacey A. Missmer, Sc.D.,

Xiuye Xing, M. D. , Ph. D. , Han Zhao, M. D. , Ph. D. , Mei Li, M. Sc

A new approach to ovarian reserve testing

DNA integrity is maintained after freeze-drying of human spermatozoa

Florian Schneider, M. D. , Klaus Redmann, Ph. D. , Joachim Wistuba, Ph

What are patients doing with their mosaic embryos

Age-specific probability of live birth with oocyte cryopreservation: an individual patient data meta-analysis Aylin Pelin Cil, M.D., Heejung Bang, Ph.D.,

Evaluation of diagnostic testis biopsy and the repetition of testicular sperm extraction surgeries in infertility patients Alayman Hussein, M.B.B.C.H.,

Ri-Cheng Chian, Ph.D., Peter S. Uzelac, M.D., Geeta Nargund, M.D.

Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles Giannina.

Effect of varying equilibration time in a two-step vitrification method on the post-warming DNA integrity of mouse blastocysts Amr Kader, M.D., Audrey.

Lindsay M. Mains, M. D. , Babak Vakili, M. D. , Yves Lacassie, M. D

L. Scott, J. Bernsten, K. DeLegge, J. Hill, N. Ramsing

Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in.

Fertility and Sterility: an evaluation

Insulin growth factors may be implicated in human sperm capacitation

Marta Baptista, M. Sc. , Stephen J. Publicover, Ph. D

Impact of subclinical hypothyroidism in women with recurrent early pregnancy loss Lia A. Bernardi, M.D., Ronald N. Cohen, M.D., Mary D. Stephenson, M.D.,

Eric S. Surrey, M. D. , Debra A. Minjarez, M. D. , John M. Stevens, B

Genetic evaluation procedures at sperm banks in the United States

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

In vitro sildenafil citrate use as a sperm motility stimulant

Saad Elzanaty, M.D., Ph.D., Johan Malm, M.D., Ph.D.

Krinos M. Trokoudes, M. D. , Constantinos Pavlides, M. Sc

CAG repeat length variation in the Androgen Receptor gene is not associated with spermatogenic failure Henrike Westerveld, M.D., Liesbeth Visser, M.Sc.,

Contact among families who share the same sperm donor

Randomized, controlled pilot trial of natural versus hormone replacement therapy cycles in frozen embryo replacement in vitro fertilization Ginny Mounce,

Myung-Geol Pang, Ph. D. , Young-Ah You, M. S. , Yoo-Jin Park, M. S

Effect of two assisted oocyte activation protocols used to overcome fertilization failure on the activation potential and calcium releasing pattern Dimitra.

In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection Sílvia Mateo,

Presentation transcript:

Variance in total levels of phospholipase C zeta (PLC-ζ) in human sperm may limit the applicability of quantitative immunofluorescent analysis as a diagnostic indicator of oocyte activation capability Junaid Kashir, Ph.D., Celine Jones, Ginny Mounce, M.Sc., Walaa M. Ramadan, M.Sc., Bernadette Lemmon, B.M., Bjorn Heindryckx, Ph.D., Petra de Sutter, M.D., Ph.D., John Parrington, Ph.D., Karen Turner, Ph.D., Tim Child, M.D., Enda McVeigh, M.D., Kevin Coward, Ph.D. Fertility and Sterility Volume 99, Issue 1, Pages 107-117.e3 (January 2013) DOI: 10.1016/j.fertnstert.2012.09.001 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Mean relative total phospholipase C zeta (PLC-ζ) fluorescence levels exhibited by sperm from individual control and oocyte activation–deficient (OAD) patients. Fluorescence intensity was quantified in arbitrary units using ImageJ software. Asterisks (*; **; ***) denote statistically significant differences (P≤.05), whereas hash marks (#; ##; ###; #′; ##′) denote statistically insignificant differences. Combinations of marks indicate comparisons between different groups (e.g., # indicates comparison with one group, and ## indicates comparison with another). Data are shown as mean ± SEM. Fertility and Sterility 2013 99, 107-117.e3DOI: (10.1016/j.fertnstert.2012.09.001) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (A) Representative confocal images of phospholipase C zeta (PLC-ζ) immunofluorescence in human sperm showing characteristic localization patterns previously identified by Grasa et al. (2008). However, additional patterns, not previously described by Grasa et al., were identified in the present study. Images were captured at ×63 optical magnification and represent overlays of PLC-ζ (green), and differential interference contrast images. White scale bars indicate 5 μm. White arrow indicates acrosomal localization; black arrow indicates equatorial localization; and red arrows indicate postacrosomal localization. The rightmost panel indicates sperm exhibiting no PLC-ζ immunoreactivity. (B) Mean proportions of sperm (percentages) exhibiting PLC-ζ immunofluorescence from control subjects and oocyte activation–deficient (OAD) patients. Proportions were determined with the use of ImageJ software. The asterisks (*; **) on the histogram denote statistically significant differences (P≤.05), whereas hash marks (#; ##; ###) denote statistically insignificant differences (P≥.05). Combinations of marks indicate comparisons between different groups (e.g., * indicates comparison with one group, and ** indicates comparison with another). Data are shown as mean ± SEM. Fertility and Sterility 2013 99, 107-117.e3DOI: (10.1016/j.fertnstert.2012.09.001) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Mean relative total phospholipase C zeta (PLC-ζ) fluorescence levels exhibited by control and oocyte activation–deficient (OAD) patient sperm. Fluorescence intensity was quantified in arbitrary units with the use of ImageJ software. Asterisk (*) denotes a statistically significant difference (P≤.05). Data are shown as mean ± SEM. Fertility and Sterility 2013 99, 107-117.e3DOI: (10.1016/j.fertnstert.2012.09.001) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Mean proportions of sperm (percentages) exhibiting phospholipase C zeta (PLC-ζ) immunofluorescence from control and oocyte activation–deficient (OAD) sperm. Proportions were determined with the use of ImageJ software. Asterisk (*) denotes a statistically significant difference (P≤.05). Data are shown as mean ± SEM. Fertility and Sterility 2013 99, 107-117.e3DOI: (10.1016/j.fertnstert.2012.09.001) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 3 Mean proportions of sperm (percentages) exhibiting phospholipase C zeta (PLC-ζ) immunofluorescence patterns in total sperm from (A) control subjects and (B) oocyte activation–deficient (OAD) patients. Proportions were determined with the use of ImageJ software. (A) The asterisk (*) denotes statistically significant differences (P≤.05), whereas hash marks (#; #′; ##; ###) on the histogram denote statistically insignificant differences (P≥.05). (B) Asterisks (*; **; **′; ***) on the histogram denote statistically significant differences (P≤.05). Combinations of asterisks and hash marks on both histograms indicate comparisons between different groups (e.g., # indicates comparison with one group, and ## indicates comparison with another). Data are shown as mean ± SEM. Fertility and Sterility 2013 99, 107-117.e3DOI: (10.1016/j.fertnstert.2012.09.001) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions