Genomic landscapes of the two typical NEs found in this study.

Slides:



Advertisements
Similar presentations
What is neural stemness? Why is it important? What are the molecular signatures of neural stemness? What are the regulatory networks that control neural.
Advertisements

Data integration across omics landscapes Bing Zhang, Ph.D. Department of Biomedical Informatics Vanderbilt University School of Medicine
Transcriptome Sequencing with Reference
발표자 석사 2 년 김태형 Vol. 11, Issue 3, , March 2001 Comparative DNA Sequence Analysis of Mouse and Human Protocadherin Gene Clusters 인간과 마우스의 PCDH 유전자.
COURSE OF BIOINFORMATICS Exam_31/01/2014 A.
The Havana-Gencode annotation GENCODE CONSORTIUM.
RNA-Seq Primer Understanding the RNA-Seq evidence tracks on the GEP UCSC Genome Browser Wilson Leung08/2014.
What do we already know ? The rice disease resistance gene Pi-ta Genetically mapped to chromosome 12 Rybka et al. (1997). It has also been sequenced Bryan.
COURSE OF BIOINFORMATICS Exam_30/01/2014 A.
Figure S2 A B Log2 Fold Change (+/- cAMP) Transcriptome (9hr)
ENCODE Pseudogenes and Transcription
DNase‐HS sites are main independent determinants of DNA replication timing Simulations based on genome sequence features (GC content, CpG islands), or.
Inferred dynamic model of transcriptional regulatory network.
binding sites 58 of the 473 unambiguously assigned phosphorylation sites are predicted by Scansite to be sites for binding. 50 of these correspond.
Principal component analysis of the GO category composition of all genes in each genome/transcriptome and WGD paralogs. Principal component analysis of.
Phosphorylation and sequence disorder in microtubule-associated protein Tau.A, schematic illustration of the domain profile of Tau with all known phosphorylation.
Distribution of phosphorylation sites identified in the cytosolic phosphoproteome.A, numbers of approved phosphopeptides, previously phosphorylated peptides,
Sequence analyses and evolutionary conservation of the CLDN10 gene and the identified CLDN10 variants. Sequence analyses and evolutionary conservation.
Relationship between Genotype and Phenotype
Effect of altered 3′UTR on miRNA-mediated gene regulation.
The upstream region of MUC5AC bound by SPDEF is required for the expression of MUC5AC in human lung cancer cells The upstream region of MUC5AC bound by.
SgRNAs targeting the 5′ coding region of β-catenin are ineffective due to an alternative translation initiation site in exon 3. sgRNAs targeting the 5′
Figure 3. Genes differentially expressed in batch cultures during adaptation to low temperature. Genes differentially expressed in batch cultures during.
Novel phosphorylation sites on H+-ATPase proteins
Diverse abnormalities manifest in RNA
The Antisense Transcriptomes of Human Cells
Figure 5 Systems biological model of IBS
Predicted location and functional classification of differentially expressed transcripts. Predicted location and functional classification of differentially.
Identification of essential genome features.
Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,
Protein information in the Human Protein Atlas.
Schematic of MS1 filtering.
Visual validation of the computational outputs.
Schematic representation of proteogenomic annotation strategy.
Evaluation to the NEs with the evidence of FNPs
Correction of translational start site by identification of N-terminal peptide. Correction of translational start site by identification of N-terminal.
Evaluation of the novel peptides derived from MS/MS data.
A, schematic presentation of fetuin-A domains.
N-terminal extension of a gene using peptides mapping upstream to an annotated start site. N-terminal extension of a gene using peptides mapping upstream.
Identification of novel peptides in annotations from other plants.
Tissue Western analysis of purified monospecific antibodies.
Testing the effectiveness of the three-step peptide fractionation method.A, μLC mass chromatograms of SCX fractions for an acidic FFE fraction. Testing.
Altered pathways in prostate cancer.
Interaction networks of the regulated phosphoproteins.
Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum. Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum.A,
Plot of the deviation of the predicted pI value of every peptide spectrum from the average pI calculated for each fraction for validated (a) and non-validated.
Multiple filtration to treat the putative novel peptides.
Comparison of mapped epitopes and peptides identified in immuno-SILAC screening of polyclonal antibodies against trypsin-digested PrESTs. Comparison of.
Number of genes/antibodies included in the database.
Bar plot representation of the transcriptomic changes in Δsaci_ptp and Δsaci_pp2a. Bar plot representation of the transcriptomic changes in Δsaci_ptp and.
Genome-wide binding sites of OsMADS1 and the distribution of binding sites in different regions of annotated genes. Genome-wide binding sites of OsMADS1.
Comparison of proteomics and RNA‐Seq data.
Illustration of chromatography metric C-2A applied to LC-MS/MS data from three Thermo LTQ systems in analyses of yeast proteome samples in CPTAC Study.
Characterising dermis expansion and gene expression changes during mouse development (related to Fig 1)‏ Characterising dermis expansion and gene expression.
Schematic of AIMS-to-MRM experiment.
Overlap between changes in de novo protein synthesis after p53- or miR-34a-induction. Overlap between changes in de novo protein synthesis after p53- or.
Integrative omic approaches for the study of host–pathogen interactions Integrative omic approaches for the study of host–pathogen interactions (A) Proteomic.
Occurrence of fur−-only genes and expected sensitivity to Fur.
Universal Alternative Splicing of Noncoding Exons
Detection of H3K9ac enrichment peaks during the 9-d timeframe of dox-induced mitDNA depletion by ChIPseq. Detection of H3K9ac enrichment peaks during the.
The genomic distribution of essential and non-essential mouse genes, separated into known and predicted essentiality. The genomic distribution of essential.
Genomic structure of LTBP-4 around the 3rd 8-Cys repeat.
Structure of Wt1 genomic regions in human and zebrafish.
Comparing drug sensitivity predictions from different data types in melanoma and endometrial cancer cell lines. Comparing drug sensitivity predictions.
Survey of phosphorylation motifs
Prophage genome map of the C. diphtheriae prophage encoding DT
Figure Genetic characterization of the novel GYG1 gene mutation (A) GYG1_cDNA sequence and position of primers used. Genetic characterization of the novel.
Cartoon illustrating the genomic organization of a typical mucosal high-risk HPV. The genome contains early and late regions, which relate to the positions.
Lack of pilE expression in NZCM238 is associated with lower TNF-α secretion by 16HBE cells during coculture. Lack of pilE expression in NZCM238 is associated.
Presentation transcript:

Genomic landscapes of the two typical NEs found in this study. Genomic landscapes of the two typical NEs found in this study.A, Both evidence types, transcriptomics and proteomics, support the encoding regions located upstream of the of the Os12t0428600 gene. A screen shot is obtained from Gramene, showing the genomic information of position 13687144–13702026 on rice chromosome 12. The track names on the left illustrate evidence from different sources, including predicted genes, NEs and novel peptides. On the right, the genomic landscape for the gene Os12t0428600 from a theoretical prediction and official annotation, and the location of the transcripts and peptides identified on the same gene based on the official annotation and the NEs. B, A screen shot is obtained from Gramene, showing the genomics information of 22413908–22418499 on rice chromosome 3. The three novel peptides and especially the junction peptide (NTLYVGGLAEEVDEK) suggest a novel encoding region and confirmed its junction site. Zhe Ren et al. Mol Cell Proteomics 2019;18:86-98 © 2019 Varland et al.