Vectors for CRISPR mutagenesis in Candida albicans, Candida glabrata, Naumovozyma castellii, and Saccharomyces cerevisiae. Vectors for CRISPR mutagenesis.

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Vectors for CRISPR mutagenesis in Candida albicans, Candida glabrata, Naumovozyma castellii, and Saccharomyces cerevisiae. Vectors for CRISPR mutagenesis in Candida albicans, Candida glabrata, Naumovozyma castellii, and Saccharomyces cerevisiae. (A) Recyclable C. albicans CRISPR vector pV1200 (previous generation) replaces one copy of ENO1, and flipout removes NATr and sgRNA gene sequences. Current vectors pV1393 and pV1524 insert into the Neut5L locus, and flipout leaves only an FRT insertion at Neut5L. Vector pV1393 uses SAP2p to drive FLP, while pV1524 uses MAL2p. (B) Vectors pV1326 and pV1382 for CRISPR mutagenesis in C. glabrata and S. cerevisiae. (C) Vector pV1465 for CRISPR mutagenesis in N. castellii. Valmik K. Vyas et al. mSphere 2018; doi:10.1128/mSphere.00154-18