Reeling in the Catch: Advancing Cleavable Linkers for Proteomics

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Presentation transcript:

Reeling in the Catch: Advancing Cleavable Linkers for Proteomics Meng M. Rowland, Michael D. Best Chemistry & Biology Volume 17, Issue 11, Pages 1166-1168 (November 2010) DOI: 10.1016/j.chembiol.2010.11.001 Copyright © 2010 Elsevier Ltd Terms and Conditions

Figure 1 Cartoon Depicting the Purification of Labeled Proteins using a Diazobenzene-Cleavable Linker Proteins that have been labeled with alkyne- (or azide-) tagged moieties are selectively derivatized using click chemistry and purified through attachment to a solid support. Cleavage of the diazobenzene linker is then used to efficiently release purified proteins to facilitate purification and analysis. Note: for a previous cartoon depicting bioorthogonal labeling using a fishing analogy, see Sletten and Bertozzi, 2009. Chemistry & Biology 2010 17, 1166-1168DOI: (10.1016/j.chembiol.2010.11.001) Copyright © 2010 Elsevier Ltd Terms and Conditions