Volume 65, Issue 2, Pages (August 2016)

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Volume 65, Issue 2, Pages 450-451 (August 2016) Corrigendum to “Interleukin-30 (IL27p28) alleviates experimental sepsis by modulating cytokine profile in NKT cells” Jun Yan, Abhisek Mitra, Jiemiao Hu, Jeffery J. Cutrera, Xueqing Xia, Thomas Doetschman, Mihai Gagea, Lopa Mishra, Shulin Li Journal of Hepatology Volume 65, Issue 2, Pages 450-451 (August 2016) DOI: 10.1016/j.jhep.2016.04.028 Copyright © 2016 European Association for the Study of the Liver Terms and Conditions

Fig. 5 IL-30 attenuates IFNγ and TNF-α production and augments IL-10 production in NKT cells of septic mice. Liver and spleen tissues were collected 6h after LPS challenge. (A, B) Intracellular cytokine staining of IFNγ+, IL-10+ and TNF-α+ lymphocytes were shown in livers and spleens respectively from mice treated with LPS plus control DNA (pCtlr) or plus IL30-encoding DNA injection (pIL30) (N=3 mice). IFNγ+, IL-10+ and TNF-α+ gating for these two panels were based on their matched fluorescence minus one (FMO) control respectively. Bar graphs represent the mean percentage of cytokine positive cells in immune cells gated population. (C, D) Cytokine positively gated cell population from panels A and B were analyzed to determine the percentage of NK (CD3-NK1.1+), NKT (CD3+ NK1.1+), T (CD3+ NK1.1-) and other immune cells (CD3-NK1.1-) in liver and spleen respectively. Gates were determined for CD3 and NK1.1 axes based on their matched fluorescence minus one (FMO) control respectively. Matched isotype staining of CD3 and NK1.1 in IFNγ+, IL-10+, or TNF-α+ cells were used as isotype control for C, D panels. Bar graph represents the number of cytokine positive NK, NKT, T and other immune cells based on the every 10E4 immune cells in livers and spleens respectively. Data are representative of three independent experiments. (This figure appears in colour on the web.) Journal of Hepatology 2016 65, 450-451DOI: (10.1016/j.jhep.2016.04.028) Copyright © 2016 European Association for the Study of the Liver Terms and Conditions