Student: Crenguța Bucătaru University of Agronomic Sciences and Veterinary Medicine of Bucharest Faculty of Horticulture International Student Symposium “Hortus Academicus” March 29, 2019, Bucharest, Romania Genetic variability increased through intra and inter-specific hybrids in goji Student: Crenguța Bucătaru Assoc.Prof.Dr. Adrian Asănică International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Origin Introduction Family Solanacee Gender Lycium Lycium chinense International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Goji Introduction Lycium chinense Vitamins: A, C, E Antioxidants,fiber, beta-carotene Fruits with nutraceutical effects: effect on high cholesterol, diabetes, heart disease, tuberculosis, infantile pneumonia, anemia, visual disturbances.. Goji Lycium chinense International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Materials and Methods Location: Experimental Field of the Faculty of Horticulture, USAMV, Bucharest Trellis system: 3 pine pillars with 3 m height 4 supporting wires at 45 cm distance between them Planting distances: 1.2 m x 1.0 m Irrigation system: drip, flow rate 2l / hour International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Goji Materials and Methods Genitors Lycium chinense Maternal parent Patternal parent 410 C9 AS S Lycium chinense International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Materials and Methods Hybrid fruit Choice of parents, flowers for breeding Flower emasculation Izolation of emasculated flowers Breeding International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Removing all petals and stamens to prevent self-pollination Flower emasculation: Removing all petals and stamens to prevent self-pollination International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Isolation of castrated flowers: Introduction of the branches with emasculated flower in paper bags closed at the both sides International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Crossing breeding realised by touching between flowers International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

The seedling of hybrid seeds International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Results and discussions Genitors: 4 Lycium sp. biotypes Maternal parent Paternal parent Pollinated pistils Hybrid fruits Seeds number Fruit weight average (g) Code 410 C9 14 3/2 AS 36 6 78 0.21 5/2 S 10 1 5 0.12 6/4 16 4 34 0.4 7/4 International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Results and discussions Parents Seed Number of rising plants Percent Matern Patern Seeds number Rising 410 C9 0 % AS 78 31 39,74 % S 5 34 28 82,35 % International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

Conclusion and reccomendation Very good results after using the direct flower touch pollination method. The capacity of pollen germination was between 10 % for biotype B1, 42% for biotype AS The calendar for the start of work must be earlier, immediately after the binding for the first series of flowers International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

References Asănică A., 2017 – Vertical training of berry shrubs, Total Publishing, Bucharest Asanica A., 2015, 2016, 2017. Innovations in the fruit bushes culture, Stage I, II and III reports, USAMV Bucharest http://www.innoberry.usamv.ro/ https://ro.wikipedia.org/wiki/C%C4%83tin%C4%83_de_garduri#Variet%C4%83%C8%9Bi_Goji https://doc.ro/dieta-si-sport/fructele-goji-beneficii-si-contraindicatii http://www.lubera.co.uk/blog/plants/turgidus-the-large-sweet-goji-berry International Student Symposium "Hortus Academicus“ March 29, 2019 - Bucharest, Romania

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