Volume 114, Issue 1, Pages 37-43 (January 1998) Association of serum antibodies against p53 protein with poor survival in patients with Zollinger–Ellison syndrome  Philippe Jaïs, Albert Vuagnat, Benoit Terris, Anne–Marie Houillier, Serge Bonfils, Pierre Laurent–Puig, Michel Mignon, Miguel J.M. Lewin  Gastroenterology  Volume 114, Issue 1, Pages 37-43 (January 1998) DOI: 10.1016/S0016-5085(98)70630-4 Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 1 ELISA anti-p53 antibody levels in controls and patients with ZES. Anti-p53 antibody positivity was defined as an absorbance above that of the positive control sample (dashed line). Gastroenterology 1998 114, 37-43DOI: (10.1016/S0016-5085(98)70630-4) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 2 Western blotting of serum anti-p53 autoantibodies. Recombinant p53 protein was subjected to 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and electrophoretically transferred onto nitrocellulose membrane. The membrane was cut into strips, and each strip was reacted with 1:100 diluted serum from (lanes 1-7) ZES patients with positive results of ELISA, (lane 8) the control patient with positive results of ELISA, and (lane 9) a control patient with negative results of ELISA. Gastroenterology 1998 114, 37-43DOI: (10.1016/S0016-5085(98)70630-4) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 3 Kaplan–Meier survival curves according to the presence or the absence of anti-p53 antibodies. The two curves were statistically different using the log rank test (P = 0.0009). Anti-p53 Ab (−), anti-p53 antibody negative; Anti-p53 Ab (+), anti-p53 antibody positive. Gastroenterology 1998 114, 37-43DOI: (10.1016/S0016-5085(98)70630-4) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 4 Kaplan–Meier survival curves of the ZES patients with or without liver metastases according to the presence or the absence of anti-p53 antibodies. M (−), Anti-p53 Ab (−), no liver metastasis and anti-p53 antibody negative; M (+), Anti-p53 Ab (−), liver metastasis and anti-p53 antibody negative; M (−), Anti-p53 Ab (+), no liver metastasis and anti-p53 antibody positive; M (+), Anti-p53 Ab (+), liver metastasis and anti-p53 antibody positive. Gastroenterology 1998 114, 37-43DOI: (10.1016/S0016-5085(98)70630-4) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 5 p53 protein nuclear staining of a gastrinoma from a patient with liver metastasis using anti-p53 antibody DO-7. Gastroenterology 1998 114, 37-43DOI: (10.1016/S0016-5085(98)70630-4) Copyright © 1998 American Gastroenterological Association Terms and Conditions