Epitope-Specific Antibody Response to Mel-CAM Induced by Mimotope Immunization Christine Hafner, Stefan Wagner, Joanna Jasinska, Dorothee Allwardt, Otto.

Slides:

Advertisements

Similar presentations

Autoantibodies in Scurfy Mice and IPEX Patients Recognize Keratin 14

Advertisements

Journal of Allergy and Clinical Immunology

Volker Assmann, Christina Fieber, Peter Herrlich, Martin Hofmann

Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin)

Antigen-Specific CD8 T Cells Can Eliminate Antigen-Bearing Keratinocytes with Clonogenic Potential via an IFN-γ-Dependent Mechanism Rachel L. De Kluyver,

Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy Raphaela.

Birgit Stallmeyer, Heiko Kämpfer, Josef Pfeilschifter, Stefan Frank

Induction of IgE antibodies with predefined specificity in rhesus monkeys with recombinant birch pollen allergens, Bet v 1 and Bet v 2 Fatima D. Ferreira,

Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes Genji Imokawa, Yutaka Takagi,

Overexpression of Laminin-8 in Human Dermal Microvascular Endothelial Cells Promotes Angiogenesis-Related Functions Jie Li, Lisa Zhou, Hoang T. Tran,

Specific Lysis of Melanoma Cells by Receptor Grafted T Cells is Enhanced by Anti- Idiotypic Monoclonal Antibodies Directed to the scFv Domain of the Receptor

IL-18 Regulates Melanoma VLA-4 Integrin Activation through a Hierarchized Sequence of Inflammatory Factors María Valcárcel, Teresa Carrascal, Olatz Crende,

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Maria A. Vidal, Angel Astroza, Carola E

Elastin-Derived Peptides Upregulate Matrix Metalloproteinase-2-ediated Melanoma Cell Invasion Through Elastin-Binding Protein Carole Ntayi, Anne-Laure.

17β-estradiol, Progesterone, and Dihydrotestosterone Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production Naoko Kanda, Shinichi.

Anti-HLA antibody binding to HLA class I molecules induces proliferation of airway epithelial cells: A potential mechanism for bronchiolitis obliterans.

Aimee S. Payne, Don L. Siegel, John R. Stanley

Xiang Ding, Luis A. Diaz, Janet A. Fairley, George J. Giudice, Zhi Liu

Volume 115, Issue 4, Pages (October 1998)

17β-Estradiol Enhances Vascular Endothelial Growth Factor Production and Dihydrotestosterone Antagonizes the Enhancement via the Regulation of Adenylate.

Selection of Mimotopes of the Cell Surface Adhesion Molecule Mel-CAM from a Random pVIII-28aa Phage Peptide Library Christine Hafner, Ursula Samwald,

Gangliosides GD1b, GT1b, and GQ1b Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production: the Inhibition of Adenylate Cyclase1

T Cell Stimulating Stratum Corneum Antigens: Characterization by Chromatography and Electrophoresis Indicates Limited Diversity Jonathan M. Hales, Richard.

Evidence that Anti-Type VII Collagen Antibodies Are Pathogenic and Responsible for the Clinical, Histological, and Immunological Features of Epidermolysis.

Function Blocking Autoantibodies Against Matrix Metalloproteinase-1 in Patients with Systemic Sclerosis Shinichi Sato, Ikuko Hayakawa, Minoru Hasegawa,

Identification of Monoclonal Anti-HMW-MAA Antibody Linear Peptide Epitope by Proteomic Database Mining Abraham Mittelman, Raj Tiwari, Guglielmo Lucchese,

S100A15, an Antimicrobial Protein of the Skin: Regulation by E

Protein unfolding strongly modulates the allergenicity and immunogenicity of Pru p 3, the major peach allergen Masako Toda, PhD, Gerald Reese, PhD, Gabriele.

Inhibitory Effect of β-Thujaplicin on Ultraviolet B-Induced Apoptosis in Mouse Keratinocytes Takako Baba, Hajime Nakano, Katsuto Tamai, Daisuke Sawamura,

In Vitro Keratinocyte Dissociation Assay for Evaluation of the Pathogenicity of Anti- Desmoglein 3 IgG Autoantibodies in Pemphigus Vulgaris Ken Ishii,

PARP Determines the Mode of Cell Death in Skin Fibroblasts, but not Keratinocytes, Exposed to Sulfur Mustard Dana Anderson, Betty Benton, Zhao-Qi Wang,

The Function of Nitric Oxide in Wound Repair: Inhibition of Inducible Nitric Oxide- Synthase Severely Impairs Wound Reepithelialization Birgit Stallmeyer,

Hermine Schlagbauer-Wadl, Marieke Griffioen, Andrea van Elsas, Peter I

Halofuginone, an Inhibitor of Type-I Collagen Synthesis and Skin Sclerosis, Blocks Transforming-Growth-Factor-β-Mediated Smad3 Activation in Fibroblasts

Interleukin-6-Resistant Melanoma Cells Exhibit Reduced Activation of STAT3 and Lack of Inhibition of Cyclin E-Associated Kinase Activity Markus Böhm,

Contact Hypersensitivity in MHC Class II-Deficient Mice Depends on CD8 T Lymphocytes Primed by Immunostimulating Langerhans Cells Anne Bouloc, Andrea.

Human Keratinocytes Respond to Osmotic Stress by p38 Map Kinase Regulated Induction of HSP70 and HSP27 M. Garmyn, A. Pupe Journal of Investigative Dermatology

Resistance of Human Melanoma Cells Against the Death Ligand TRAIL Is Reversed by Ultraviolet-B Radiation via Downregulation of FLIP Elke Zeise, Michael.

Transforming Growth Factor β1 Induces Apoptosis in Normal Melanocytes but not in Nevus Cells Grown in Type I Collagen Gel Tuomo Alanko Journal of Investigative.

Journal of Investigative Dermatology

IFN-τ inhibits IgE production in a murine model of allergy and in an IgE-producing human myeloma cell line Mustafa G. Mujtaba, PhDa, Lorelie Villarete,

Increased Expression of Endothelial Cell Adhesion Molecules Due to Mediator Release from Human Foreskin Mast Cells Stimulated by Autoantibodies in Chronic.

Belén López-García, Phillip H. A. Lee, Kenshi Yamasaki, Richard L

Society for Investigative Dermatology 2010 Meeting Minutes

Erythema Multiforme Associated Human Autoantibodies Against Desmoplakin I and II: Biochemical Characterization and Passive Transfer Studies Into Newborn.

The Intracellular and Extracellular Domains of BP180 Antigen Comprise Novel Epitopes Targeted by Pemphigoid Gestationis Autoantibodies Giovanni Di Zenzo,

Interferon-γ-Mediated Growth Regulation of Melanoma Cells: Involvement of STAT1- Dependent and STAT1-Independent Signals Anja Bosserhoff Journal of Investigative.

Yohei Mukoyama, Shuxia Zhou, Yoshiki Miyachi, Norihisa Matsuyoshi

Research Snippets Journal of Investigative Dermatology

Malondialdehyde oxidation of cartilage collagen by chondrocytes

Mads Hald Andersen, Jürgen C. Becker, Per thor Straten

Research Snippets from the British Journal of Dermatology

Pathogenicity and Epitope Characteristics of Anti-Desmoglein-1 from Pemphigus Foliaceus Patients Expressing Only IgG1 Autoantibodies Mary K. Hacker-foegen,

Dawn Cooper, Jonathan Hales, Richard Camp

Journal of Investigative Dermatology

Autoantibody in Mucous Membrane Pemphigoid Binds to an Intracellular Epitope on Human β4 Integrin and Causes Basement Membrane Zone Separation in Oral.

Autoantibodies to BP180 Associated with Bullous Pemphigoid Release Interleukin-6 and Interleukin-8 from Cultured Human Keratinocytes Enno Schmidt, Stanislaus.

Identification of Androgen-Inducible TGF-β1 Derived from Dermal Papilla Cells as a Key Mediator in Androgenetic Alopecia Shigeki Inui, Yoko Fukuzato,

A hypoallergenic cat vaccine based on Fel d 1–derived peptides fused to hepatitis B PreS Katarzyna Niespodziana, MSc, Margarete Focke-Tejkl, PhD, Birgit.

Valerie R. Wiersma, Marco de Bruyn, Robert J

Janet A. Fairley, Chang Ling Fu, George J. Giudice

Human Leukocyte Elastase Induces Keratinocyte Proliferation by Epidermal Growth Factor Receptor Activation Ulf Meyer-Hoffert, Jana Wingertszahn, Oliver.

Journal of Investigative Dermatology

Volume 57, Issue 2, Pages (October 2000)

Envoplakin and Periplakin, the Paraneoplastic Pemphigus Antigens, are also Recognized by Pemphigus Foliaceus Autoantibodies Shideh Kazerounian, Mỹ G.

Pegylated and Conventional Interferon-α Induce Comparable Transcriptional Responses and Inhibition of Tumor Growth in a Human Melanoma SCID Mouse Xenotransplantation.

Histone H4 Is a Major Component of the Antimicrobial Action of Human Sebocytes Dong-Youn Lee, Chun-Ming Huang, Teruaki Nakatsuji, Diane Thiboutot, Sun-Ah.

Zhuxiang Nie, Takashi Hashimoto Journal of Investigative Dermatology

Jay E. Slater, MDa, Elizabeth J. Paupore, BSa, Michael R

Presentation transcript:

Epitope-Specific Antibody Response to Mel-CAM Induced by Mimotope Immunization Christine Hafner, Stefan Wagner, Joanna Jasinska, Dorothee Allwardt, Otto Scheiner, Klaus Wolff, Hubert Pehamberger, Ursula Wiedermann, Heimo Breiteneder Journal of Investigative Dermatology Volume 124, Issue 1, Pages 125-131 (January 2005) DOI: 10.1111/j.0022-202X.2004.23515.x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Development of mimotope-specific antibodies. Microtiter plates were coated with melcam mim1 and melcam mim2 and an ELISA assay was performed to measure specific antibody levels. Sera of animals (n=5) immunized with tetanus toxoid (TT)-melcam mim1 and TT as control were analyzed for (A) IgG1 and (B) IgG2a subclasses in dilutions from 1:640 to 1:40,640 in triplicates. Sera of animals (n=5) immunized with TT-melcam mim2 were also analyzed for (C) IgG1 and (D) IgG2a subclasses in dilutions from 1:640 to 1:40,640 in triplicates. □, pre-immune sera; ▪, immune sera. Journal of Investigative Dermatology 2005 124, 125-131DOI: (10.1111/j.0022-202X.2004.23515.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Melanoma cell-adhesion molecule (Mel-CAM)-specific ELISA inhibition assay. ELISA was performed to evaluate antibodies specific to recombinant Mel-CAM after immunization with (A) melcam mim1 and melcam mim2 and (B) tetanus toxoid-coupled melcam mim1 and melcam mim2. Sera were analyzed in a dilution 1:40 or 1:500, respectively. Sera were pre-incubated with increasing concentrations of melcam mim1, melcam mim2, or an unrelated peptide ch3 as the inhibitor, and incubated with recombinant Mel-CAM coated to a microtiter plate. Binding results are shown as inhibition (percentage) of binding of mouse sera to Mel-CAM as a function of the free mimotope concentration. ▪, melcam mim1 serumpool/inhibition with melcam mim1; □, melcam mim2 serumpool/inhibition with melcam mim2; △, melcam mim1 serumpool/inhibition with ch3. Journal of Investigative Dermatology 2005 124, 125-131DOI: (10.1111/j.0022-202X.2004.23515.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Melanoma cell-adhesion molecule (Mel-CAM)-specific immunoblot. Eight micrograms of Mel-CAM were separated by 8% SDS-PAGE, transferred to a nitrocellulose membrane and incubated with a pre-immune serum pool (lane 1), a serum pool from melcam mim1-immunized mice (lane 2), melcam mim2-immunized mice (lane 3), tetanus toxoid (TT)-coupled melcam mim1-immunized mice (lane 4) and TT-coupled melcam mim2-immunized mice (lane 5), monoclonal antibody MAd18-5D7 (lane 6), and TT-immunized mice (lane 7). (B) Buffer control, (C) isotype control; arrow indicates position of recombinant Mel-CAM. Journal of Investigative Dermatology 2005 124, 125-131DOI: (10.1111/j.0022-202X.2004.23515.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Cell proliferation assay on splenocytes of mice immunized with the indicated mimotope or tetanus-coupled mimotope. Splenocytes were co-cultured with (A) melcam mim1or (B) melcam mim2. SI, stimulation index=ratio of mean proliferation with peptide and medium control values. Journal of Investigative Dermatology 2005 124, 125-131DOI: (10.1111/j.0022-202X.2004.23515.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Melanoma cell proliferation assay.3H-thymidine proliferation assay demonstrating the effects of the murine IgG on MelJuso and MD3a cell line growth. Journal of Investigative Dermatology 2005 124, 125-131DOI: (10.1111/j.0022-202X.2004.23515.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions