Slc1a3-CreER as a Targeting Tool for the K6+ Epithelial Stem Cell Niche and its Precursors during Mouse Hair Follicle Cycle Aiko Sada, Prachi Jain, Sherry.

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Slc1a3-CreER as a Targeting Tool for the K6+ Epithelial Stem Cell Niche and its Precursors during Mouse Hair Follicle Cycle Aiko Sada, Prachi Jain, Sherry Wang, Eva Leung, Tudorita Tumbar Journal of Investigative Dermatology Volume 137, Issue 7, Pages 1569-1571 (July 2017) DOI: 10.1016/j.jid.2017.02.974 Copyright © 2017 The Authors Terms and Conditions

Figure 1 Slc1a3-CreER specifically marks the inner root sheath in the anagen follicle. (a) Scheme for lineage tracing of Slc1a3-CreER marked cells. (b) Structure of the anagen hair follicle. Appropriate markers for each layer are shown in boxed text. (c) Immunostaining with lineage markers indicated in (b). Upper panels show sagittal sections and lower panels show cross sections of the hair follicle. The dashed line indicates the bulge. Arrowheads represent the boundary between the upper and lower IRS. Scale bars = 50 μm. A, anagen; C, catagen; Ch, cuticle of hair shaft; Ci, cuticle of IRS; Co, cortex of hair shaft; Cp, companion layer; DP, dermal papilla; He, Henle’s layer; HF, hair follicle; HS, hair shaft; Hu, Huxley’s layer; IFE, inter-follicular epidermis; IRS, inner root sheath; M, morphogenesis; Me, medulla; ORS, outer root sheath; PD, postnatal day; T, telogen. Journal of Investigative Dermatology 2017 137, 1569-1571DOI: (10.1016/j.jid.2017.02.974) Copyright © 2017 The Authors Terms and Conditions

Figure 2 Slc1a3-CreER marked cells are a source for the telogen bulge K6+ inner layer. (a–f) Images of immunostained lineage traced skin tamoxifen injected as shown in Figure 1 at ages and stages indicated. Confocal images of Z-stacks are shown in Supplementary Figure S3a–d. Arrowheads in (b) represent cell death. The dashed line in (b) represents the lower part of the follicle, where tdTomato+ cells were not colocalized with K6. The dashed line in (d) bounds the anagen follicle. Note localization of tdTomato+ cells in the old bulge in this image. The asterisk in (f) shows remnants of tdTomato+ cells shed through the infundibulum. Arrows represent tdTomato+ clones in the interfollicular epidermis. Scale bars = 50 μm. (g) Summary cartoon with tdTomato in red and K6 in green. Abbreviations are the same as in the Figure 1 legend. Journal of Investigative Dermatology 2017 137, 1569-1571DOI: (10.1016/j.jid.2017.02.974) Copyright © 2017 The Authors Terms and Conditions