Investigation of the microbiota of the reproductive tract in women undergoing a total hysterectomy and bilateral salpingo-oopherectomy  Shana M. Miles, M.D., Ph.D., Britney L. Hardy, B.S., D.Scott Merrell, Ph.D.  Fertility and Sterility  Volume 107, Issue 3, Pages 813-820.e1 (March 2017) DOI: 10.1016/j.fertnstert.2016.11.028 Copyright © 2016 Terms and Conditions

Figure 1 Distribution of phyla by body site. Samples from each patient are grouped by individual body site. Each column represents the percent abundance of the indicated bacterial phyla present at each designated body site for an individual patient. Data for the five most common phyla are presented; all remaining phyla were grouped and classified as “other.” Fertility and Sterility 2017 107, 813-820.e1DOI: (10.1016/j.fertnstert.2016.11.028) Copyright © 2016 Terms and Conditions

Figure 2 Distribution of genus by body site. Each column represents the percent abundance of the six most common genera present at a single body site from each patient. Less common genera and sequences that could not be classified to the genus level were grouped as “other.” (A) Vagina; (B) cervix; (C) endometrium; (D) myometrium; (E) fallopian tube; and (F) ovary. Fertility and Sterility 2017 107, 813-820.e1DOI: (10.1016/j.fertnstert.2016.11.028) Copyright © 2016 Terms and Conditions

Figure 3 PCoA demonstrating membership variation as determined using the Jaccard diversity calculator. To prevent potential bias from samples with few sequencing reads, only samples (33/64) with a Good's coverage value of ≥90% were included for analysis. Each colored symbol corresponds to an individual sample as indicated. The variation represented by each axis (PC1 or PC2) is indicated as %. (A) Samples grouped by patient. (B) Samples grouped by body site. Fertility and Sterility 2017 107, 813-820.e1DOI: (10.1016/j.fertnstert.2016.11.028) Copyright © 2016 Terms and Conditions

Supplemental Figure 1 PCoA demonstrating membership and abundance variation as determined using the ThetaYC diversity calculator. To prevent potential bias from samples with few sequencing reads, only samples (33/64) with a Good's coverage value of ≥90% were included for analysis. Each colored symbol corresponds to an individual sample as indicated. The variation represented by each axis (PC1 or PC2) is indicated as %. (A) Samples grouped by patient. (B) Samples grouped by body site. Fertility and Sterility 2017 107, 813-820.e1DOI: (10.1016/j.fertnstert.2016.11.028) Copyright © 2016 Terms and Conditions