Tetramer staining performed to determine frequencies of epitope-specific T cells after clearance of HPV16 infection but prior to isolation of T-cell clones.

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Tetramer staining performed to determine frequencies of epitope-specific T cells after clearance of HPV16 infection but prior to isolation of T-cell clones for subject B. Cells were selected using a lymphocyte gate in forward and side scatter and for being CD4/14/19 negative. Tetramer staining performed to determine frequencies of epitope-specific T cells after clearance of HPV16 infection but prior to isolation of T-cell clones for subject B. Cells were selected using a lymphocyte gate in forward and side scatter and for being CD4/14/19 negative. (a) Tetramer staining of PBMCs from a healthy donor known to be HLA-A6801 negative mixed with HPV16 E7 133-142 T-cell clone cells (#83-B) at a 10:1 ratio (positive control). (b) Tetramer staining of PBMCs from a healthy donor known to be HLA-A6801 negative (negative control). (c) Tetramer staining of peptide-stimulated PBMC sample collected 4 months after the clearance of HPV infection. (d) Tetramer staining of peptide-stimulated PBMC sample collected 22 months after the clearance. Xuelian Wang et al. Clin. Vaccine Immunol. 2008; doi:10.1128/CVI.00404-07