Figure 1. Structure of resistance islands containing dfrA35 in IncC plasmids. (a) pEc158 resistance island and (b) ... Figure 1. Structure of resistance.

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Figure 1. Structure of resistance islands containing dfrA35 in IncC plasmids. (a) pEc158 resistance island and (b) ... Figure 1. Structure of resistance islands containing dfrA35 in IncC plasmids. (a) pEc158 resistance island and (b) INF164 resistance island, representing the four IncC plasmids from 2013. The thick central lines represent the island, and the flanking plasmid backbone sequence is represented by the nucleotide sequence of the adjacent 5 bp at either end. Arrows indicate the extent and orientation of genes and ORFs with the names indicated below. Resistance genes are shown in red and mer genes are purple. IS are shown as green or light blue boxes with an arrow representing the transposase gene inside, and other transposition genes are dark blue. CR1 is orange with a bar representing the ori end. Gene cassettes in the integron are narrow open boxes with a bar representing the attC site and an open bar represents the attI site. The intI1 gene is light green. Vertical bars indicate inverted repeats (IR) with a label above/below and the IS4321-like elements are shown above the 38 bp IR they interrupt. A black line above the central line identifies the source of segments of the resistance island. A red bar below represents the region cloned from pEc158. This figure appears in colour in the online version of JAC and in black and white in the print version of JAC. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) J Antimicrob Chemother, dkz148, https://doi.org/10.1093/jac/dkz148 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 2. Maximum likelihood tree of DfrA amino acid sequences, generated using MEGA (version 7.0.26). Branch numbering ... Figure 2. Maximum likelihood tree of DfrA amino acid sequences, generated using MEGA (version 7.0.26). Branch numbering represents percentage bootstrap support resampled 1000 times. The scale refers to the number of substitutions per site. DfrA35 is indicated in bold. GenBank accession numbers for the DNA sequences from which amino acid sequences were obtained are located next to the protein name. The proteins used are the same as those that could be obtained from accessions in ResFinder (accessed 24 November 2018), except for the following. The DfrA19 sequence has been used for both DfrA18 and DfrA19 in ResFinder and the correct accession for dfrA18 is AY034138. DfrA3b (AY162283), which is no longer available in ResFinder, is renamed DfrA11. DfrA13 (Z50802) has not been included as it is DfrA21 with a change to the sequence of a short segment of the protein caused by two frameshift mutations. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) J Antimicrob Chemother, dkz148, https://doi.org/10.1093/jac/dkz148 The content of this slide may be subject to copyright: please see the slide notes for details.