Volume 122, Issue 1, Pages 85-93 (January 2002) Endogenous cannabinoids: A new system involved in the homeostasis of arterial pressure in experimental cirrhosis in the rat  Josefa Ros, Joan Clària, Jordi To–Figueras, Anna Planagumà, Pilar Cejudo–Martín, Guillermo Fernández–Varo, Raúl Martín–Ruiz, Vicente Arroyo, Francisca Rivera, Juan Rodés, Wladimiro Jiménez  Gastroenterology  Volume 122, Issue 1, Pages 85-93 (January 2002) DOI: 10.1053/gast.2002.30305 Copyright © 2002 American Gastroenterological Association Terms and Conditions

Fig. 1 Effect of blood cell suspension (A) or isolated monocytes (B) from control and cirrhotic rats with ascites on MAP in normal recipient rats. (C) The effect of isolated monocytes from control and cirrhotic rats with ascites on MAP in normal recipient rats pretreated with the CB1 receptor antagonist, SR141716A. (a) P < 0.05, (b) P < 0.01, and (c) P < 0.001 from corresponding control value (unpaired Student t test). Gastroenterology 2002 122, 85-93DOI: (10.1053/gast.2002.30305) Copyright © 2002 American Gastroenterological Association Terms and Conditions

Fig. 2 Effect of isolated monocytes from control (CT) and cirrhotic rats with ascites (CH) on mean arterial pressure (MAP) in normal recipient rats receiving a constant infusion of the NO synthesis inhibitor, L-NAME. (a) P < 0.05, (b) P < 0.01, and (c) P < 0.001 from rats only receiving L-NAME (unpaired Student t test). Gastroenterology 2002 122, 85-93DOI: (10.1053/gast.2002.30305) Copyright © 2002 American Gastroenterological Association Terms and Conditions

Fig. 3 Effect of blood cell suspension from hemorrhaged (○) control and (●) cirrhotic rats with ascites on mean arterial pressure (MAP) in normal recipient rats. (a) P < 0.05, (b) P < 0.01, and (c) P < 0.001 from corresponding control value (unpaired Student t test). Gastroenterology 2002 122, 85-93DOI: (10.1053/gast.2002.30305) Copyright © 2002 American Gastroenterological Association Terms and Conditions

Fig. 4 HPLC chromatograms of (A) authentic anandamide, (B) LPS-treated monocytes, (C) monocytes from control, and (D) cirrhotic rats. The column eluate was monitored by a photodiode array detector settled at 204 nm. The arrow denotes the elution position of authentic anandamide. The double-headed arrows denote the anandamide elution region. Representative chromatograms of experiments repeated 3 times are shown. Gastroenterology 2002 122, 85-93DOI: (10.1053/gast.2002.30305) Copyright © 2002 American Gastroenterological Association Terms and Conditions

Fig. 5 Identification of anandamide in rat monocytes of cirrhotic rats by GC/MS. Following HPLC purification of authentic anandamide, LPS-treated monocytes, and monocytes from control and cirrhotic rats, fractions 46 to 50 were pooled and subjected to GC/MS analysis. The chromatograms in the upper panels reflect combined current obtained by selected ion monitoring on m/z 116, m/z 175, m/z 404, and m/z 412, corresponding to the major fragments of anandamide (116/175/404) and deuterated d8-anandamide (116/175/412). The arrows denote the retention time of anandamide. The lower panels depict the SIM mass spectra at the apex of the anandamide peak. Representative chromatograms of experiments repeated 3 times are shown. Gastroenterology 2002 122, 85-93DOI: (10.1053/gast.2002.30305) Copyright © 2002 American Gastroenterological Association Terms and Conditions