Figure 1. Glc-TOR signaling is compromised in prr579 mutant

Slides:

Advertisements

Similar presentations

Figure 1. Schematic of the corA leader mRNA

Advertisements

Figure S1 A B C D E F G Long Day Hypocotyl lenght (mm)

Figure 1. Gene expression analysis

Figure 5 ISOX and vorinostat partially restore splicing pattern in DM1 patient-derived fibroblasts. (A) ISOX and vorinostat partially rescue mis-splicing.

Figure 1. Inhibition of GSK3β reduces MiR biogenesis through repression of pri-MiR processing. (A) qRT-PCR analysis of miR-27a, miR-23a, miR-24, miR-141.

Figure 1. drb7.2 mutant plants display altered accumulation of endoIR-siRNA. Wild-type (Col-0) and drb7.2 mutant plants were subjected to high throughput.

Figure 1. DNMT3A interacts with the histone deiminase PADI4

Figure 1. Cdc48 is cotranscriptionally recruited on active genes

Figure 1. Distinct chromatin regions isolated by the N-ChroP strategy

Jun-Ho Ha, Hyo-Jun Lee, Jae-Hoon Jung, Chung-Mo Park

Volume 21, Issue 2, Pages (January 2011)

Figure 1. (A) The VEGF promoter PQS and scheme of G oxidation to OG, as well as (B) the proposed APE1-dependent pathway ... Figure 1. (A) The VEGF promoter.

Figure 1. A novel image analysis tool to monitor epigenetic changes in spatiotemporal distribution of chromatin in live ... Figure 1. A novel image analysis.

Figure 1. Flow chart illustrating the experimental strategy used for the mass spectrometry analyses. Two cell ... Figure 1. Flow chart illustrating the.

Figure 1. Apparent translational control of ribi genes in the Yeast Metabolic Cycle and meiosis. (A) Harvesting scheme ... Figure 1. Apparent translational.

Figure 1. Overview of the workflow of NetworkAnalyst 3.0.

Figure 1. (A) Diagram of artificial microDNA creation by LAMA

Volume 7, Issue 9, Pages (September 2014)

Figure 7. Primary cells from prostate tumours are more sensitive to ML than adjacent non-cancerous cells from the ... Figure 7. Primary cells from.

Figure 4. (A) Venn diagram showing the overlap of peaks differentially changed in DHT as compared to NT with peaks ... Figure 4. (A) Venn diagram showing.

Figure 2. Status of rRNA methylations upon SAM depletion

Figure 1. AML containing the t(8:21) are extremely sensitive to CDK inhibitors. (A–C) Alamar blue assays were used to ... Figure 1. AML containing the.

Figure 1. Effect of random T/A→dU/A substitutions on transcription by T7 RNAP using a 321 bp DNA transcription template ... Figure 1. Effect of random.

Figure 1. Site-specific replication fork stalling at Tus/Ter barriers causes localized mutagenesis. (A) Schematic ... Figure 1. Site-specific replication.

Figure 1. Position and number of NLS improves genome editing by AsCas12a, LbCas12a and FnoCas12a. (A) General schematic ... Figure 1. Position and number.

Figure 1. Domain organization of IMP1

Figure 2. Ectopic expression of PLZF modulates H3K27ac at enhancer regions in myeloid cells. (A) Box plots showing ... Figure 2. Ectopic expression of.

Figure 6. HU sensitivity is due to the failure to process multiple consecutive ribonucleotides. 10-fold serial ... Figure 6. HU sensitivity is due to the.

BZR1 Positively Regulates Freezing Tolerance via CBF-Dependent and CBF- Independent Pathways in Arabidopsis Hui Li, Keyi Ye, Yiting Shi, Jinkui Cheng,

Figure 1. BRCA1-associated R-Loop accumulation at a non-coding region upstream of ESR1 locus. (A) Alignment of DRIP-seq ... Figure 1. BRCA1-associated.

Figure 1. Designing a cell-specific Cas-ON switch based on miRNA-regulated anti-CRISPR genes. (A) Schematic of the ... Figure 1. Designing a cell-specific.

Volume 26, Issue 14, Pages (July 2016)

The WUSCHEL Related Homeobox Protein WOX7 Regulates the Sugar Response of Lateral Root Development in Arabidopsis thaliana Danyu Kong, Yueling Hao, Hongchang.

Figure 1. Ratios of observed to expected numbers of exon boundaries aligning to boundaries of domain and disorder ... Figure 1. Ratios of observed to expected.

Figure 2. ChvR expression is controlled by the ChvI-ChvG TCS

Young-Hee Cho, Sang-Dong Yoo, Jen Sheen Cell

Figure 5. Set1-catalyzed H3K4me3 regulates histone gene transcription

Figure 1. Chemical structures of DNA and tc-DNA

Figure 1. Genetic tools used for perturbing cellular ppGpp levels in this study. The wild type E. coli cell has two ... Figure 1. Genetic tools used for.

Volume 9, Issue 1, Pages (January 2016)

The Arabidopsis Transcription Factor AtTCP15 Regulates Endoreduplication by Modulating Expression of Key Cell-cycle Genes Li Zi-Yu , Li Bin , Dong Ai-Wu.

Arabidopsis MSBP1 Is Activated by HY5 and HYH and Is Involved in Photomorphogenesis and Brassinosteroid Sensitivity Regulation Shi Qiu-Ming , Yang Xi.

Figure 1 Large consanguineous pedigree from Northern Pakistan

Volume 3, Issue 3, Pages (March 2013)

Figure 1. Illustration of DGR systems and their prediction using myDGR

Figure 1. The pipeline of Aggrescan3D 2.0 server.

Figure 1. Summary of experimental conditions and data normalization

Figure 1. Using Voronoi tessellation to define contacts

Arabidopsis WRKY45 Interacts with the DELLA Protein RGL1 to Positively Regulate Age-Triggered Leaf Senescence Ligang Chen, Shengyuan Xiang, Yanli Chen,

Figure 1. Designed cotranscriptional RNA structures

Figure 4. RLS spectra of (A) TMPipEOPP and (B) OMHEPzEOPP in the presence of different concentrations of KRAS. The RLS ... Figure 4. RLS spectra of (A)

Arabidopsis NF-YCs Mediate the Light-Controlled Hypocotyl Elongation via Modulating Histone Acetylation Yang Tang, Xuncheng Liu, Xu Liu, Yuge Li, Keqiang.

Figure 1. Denr knockdown engenders ribosome redistribution from CDS to 5′ UTR. (A) Experimental approach of the study. ... Figure 1. Denr knockdown engenders.

Xiang Han, Hao Yu, Rongrong Yuan, Yan Yang, Fengying An, Genji Qin

HOS1 Facilitates the Phytochrome B-Mediated Inhibition of PIF4 Function during Hypocotyl Growth in Arabidopsis Ju-Heon Kim, Hyo-Jun Lee, Jae-Hoon Jung,

Figure 1. Uncertainty reduction, value creation, and appropriation in two case studies. Unless provided in the caption above, the following copyright applies.

BZR1 Interacts with HY5 to Mediate Brassinosteroid- and Light-Regulated Cotyledon Opening in Arabidopsis in Darkness Qian-Feng Li, Jun-Xian He Molecular.

Figure 1. The configuration menu allows the user to choose among the main visualization/processing options of the ... Figure 1. The configuration menu.

Figure 1. ULS1 deletion causes sensitivity to ACF due Top2 activity

Figure 1. (A) Architecture of Doc2Hpo. (B) Interactive user interface

Figure 1. Overview of features that can be assessed in a single RegulationSpotter VCF analysis run. Depending upon a ... Figure 1. Overview of features.

Figure 1. Accumulation kinetics of TC-NER factors reveal a CSA independent UVSSA recruitment. (A) Representative images ... Figure 1. Accumulation kinetics.

Figure 4. MTase JHP1050 inactivation causes phenotypic effects that vary between strains: growth, viability and ... Figure 4. MTase JHP1050 inactivation.

Volume 3, Issue 2, Pages (March 2010)

Figure 1. 3C analysis of HEM3, BLM10, and SEN1 genes in rpb4Δ and isogenic wild type cells. (A) Schematic ... Figure 1. 3C analysis of HEM3, BLM10, and.

Figure 1. CSB does not affect the recruitment of OGG1 to oxidative DNA damage. (A) Representative stills of time-lapse ... Figure 1. CSB does not affect.

Arabidopsis ABF3 and ABF4 Transcription Factors Act with the NF-YC Complex to Regulate SOC1 Expression and Mediate Drought-Accelerated Flowering Keumbi.

Volume 15, Issue 1, Pages (July 2008)

The bHLH Transcription Factors MYC2, MYC3, and MYC4 Are Required for Jasmonate- Mediated Inhibition of Flowering in Arabidopsis Houping Wang, Yang Li,

Volume 11, Issue 7, Pages (July 2018)

Presentation transcript:

Figure 1. Glc-TOR signaling is compromised in prr579 mutant Figure 1. Glc-TOR signaling is compromised in prr579 mutant. (A) Venn digram showing overlap between genes activated by ... Figure 1. Glc-TOR signaling is compromised in prr579 mutant. (A) Venn digram showing overlap between genes activated by TOR signaling (9) and genes down regulated in prr579 mutant (39). (B) Compared to the WT (Col-0), primary root growth was reduced in prr579 after sugar-induced reactivation of TOR-signaling. Three-day-old seedlings grown in liquid sugar-free 1/2 MS were treated with 15 mM Glc or Suc for 3 days in weak light (22°C, LD). Red arrows indicate the hypocotyl and root junctions. DAG, days after germination; D, day; Mock, sugar-free; Glc, glucose; Suc, sucrose; (scale bar: 0.5 cm). (C) Quantitative analysis of primary root length in (B). Data represent mean ± s.e.m. (n = 18). The experiment was carried out with three biological replicates with similar results. The lengths of primary roots were measured by using ImageJ. (D, E) Root meristem proliferation was not fully activated in prr579 mutant after activation of TOR signaling by sugars. (D) Differential interference contrast (DIC) imaging of root meristems (scale bars: 50 μm). The blue arrow indicates the root quiescent cells, and the red arrow indicates the transition between meristem zone and elongation zone. (E) Quantitative analysis of root meristem length in (D). Data represent mean ± s.e.m. (n = 13). The lengths of root meristems were measured by using ImageJ. (F) The prr579 mutant was defective in S-phase entry of cell cycle as evidenced by reduced 5-ethynyl-2′-deoxyuridine staining (EdU) signals in situ (scale bars: 50 μm). (G) Time-course expression of TOR in Col-0 and prr579. The total RNA was isolated from the roots of 6-day-old seedlings grown in half-strength liquid MS medium without sugar in 12-h weak light (13 μmol m<sup>−1</sup> s<sup>−1</sup>)/12-h dark cycles at 22°C. The gene expression level was normalized by the geometric mean of ACT2 and TUB4expression. Data represent mean ± s.e.m. of three biological replicates. (H) Expression of S-phase marker genes was reduced in prr579 mutant. For RNA extraction, the roots were harvested from the seedlings treated with 15 mM Glc or Suc for 3 days after growing in liquid sugar-free 1/2 MS for 3 days in weak light (22°C, LD) at ZT8. The gene expression level was normalized by ACT2 expression. The bar values represent the gene expression levels in sugar treatments relative to the corresponding expression levels in sugar-free condition. Data represent mean ± s.e.m. of three biological repeats. Different letters in (C) and (E) represent significant difference at P < 0.01 by one-way ANOVA using SPSS software. Uppercase letters compare with each other in 15 mM Glc treatment condition, and lowercase letters compare with each other in 15 mM Suc treatment condition. The asterisk in (G) and (H) indicates significant difference as * P < 0.05, ** P < 0.01, and *** P < 0.001 by t-test. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz191, https://doi.org/10.1093/nar/gkz191 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 2. TZF1 is a direct transcriptional target of PRR5, 7 and 9 Figure 2. TZF1 is a direct transcriptional target of PRR5, 7 and 9. (A) RT-qPCR analysis of TZF1 expression in Col-0 ... Figure 2. TZF1 is a direct transcriptional target of PRR5, 7 and 9. (A) RT-qPCR analysis of TZF1 expression in Col-0 and prr579 mutant. Ten-day-old seedlings grown in LD condition were transferred to the LL condition or DD condition. The samples were collected every 3 hours over a 24-h time-course. The gene expression level was normalized by the geometric mean of ACT2 and TUB4 expression. Data represent mean ± s.e.m. of three biological repeats. (B) Diagram depicting TZF1 gene structure and the potential PRR proteins binding G-box elements in TZF1 promoter. Red arrows indicate the locations of oligo primers used for ChIP-qPCR analysis. (C-E) ChIP-qPCR results showing enriched DNA fragments containing G-box element. Ten-day-old seedlings of Col-0, PRR5pro:PRR5-GFP (ZT10), PRR7pro:PRR7-GFP (ZT8), PRR9pro:PRR9-GFP (ZT4) grown at 22°C under LD condition were used for chromatin immunoprecipitation. Error bars represent standard error of three biological replicates. The asterisk indicates significant difference relative to Col-0 at * P < 0.05 by t-test. The amplicon of APX3 promoter was used as a negative control. (F) TZF1 expression was repressed by PRR proteins in protoplast transient expression analyses. Data represent mean ± s.e.m. GUS expression was used as transformation control to normalize the LUC expression from the reporter. Error bars represent standard errors of three technical replicates. The result was shown from one of three independent experiments with similar results. The asterisk indicates significant difference as *** P < 0.001 by t-test. (G) Transient expression analysis using N. benthamiana leaf infiltration showing that TZF1 expression was repressed by PRR proteins. CCA1pro:LUC was used as a positive control for the repression by PRRs. The result was shown from one of three independent experiments with similar results. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz191, https://doi.org/10.1093/nar/gkz191 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 3. Glc-TOR signaling is compromised in TZF1 OE line Figure 3. Glc-TOR signaling is compromised in TZF1 OE line. (A) The diminished activation of Glc-TOR signaling in TZF1 ... Figure 3. Glc-TOR signaling is compromised in TZF1 OE line. (A) The diminished activation of Glc-TOR signaling in TZF1 OE line was evidenced by reduced primary root elongation. Three-day-old seedlings grown in liquid sugar-free 1/2 MS were treated with 15 mM Glc or Suc for 3 days in weak light (22°C, LD). Red arrows indicate the hypocotyl and root junctions. DAG, days after germination; D, day; Mock, sugar-free; Glc, glucose; Suc, sucrose; (scale bar: 0.5 cm). (B) Quantitative analysis of primary root length in (A). Data represent mean ± s.e.m. of 18 plants. The result was shown from one of three independent experiments with similar results. Root lengths were measured by using ImageJ. (C) DIC imaging of the root meristem zones of Col-0 and TZF1 OE line (scale bars: 50 μm). The blue arrow indicates the root quiescent cells, and the red arrow indicates the transition between meristem zone and elongation zone. (D) Quantitative analysis of the root meristem size with or without sugar treatment. Data represent mean ± s.e.m. of 13 plants. The result was shown from one of three independent experiments with similar results. The root meristem size was measured by using ImageJ. (E) S-phase entry of cell cycle in primary roots is reduced in TZF1 OE as evidenced by reduced EdU staining signals in situ (scale bar: 50 μm). (F) Time-course expression of TOR in Col-0 and TZF1 OE. The gene expression level was normalized by the geometric mean of ACT2 and TUB4 expression. The total RNA was isolated from the roots of 6-day-old seedlings grown in half-strength liquid MS medium without sugar in 12-h weak light (13 μmol m<sup>−1</sup> s<sup>−1</sup>)/12-h dark cycles at 22°C. Data represent mean ± s.e.m. of three biological replicates. (G) Expression of S-phase marker genes was reduced in TZF1 OE with sugar treatments. For RNA extraction, the roots were harvested from the seedlings treated with 15 mM Glc or Suc for 3 days after growing in liquid sugar-free 1/2 MS for 3 days in weak light (22°C, LD) at ZT8. The gene expression level was normalized by ACT2 expression. The bar value represented the gene expression levels in sugars treatment relative to the corresponding expression levels in sugar-free condition. Data represent mean ± s.e.m. of three biological repeats. Different letters in (B) and (D) represent significant difference at P < 0.01 by one-way ANOVA using SPSS software. Uppercase letters compare with each other in 15 mM Glc treatment condition, and lowercase letters compare with each other in 15 mM Suc treatment condition. The asterisk in (F) and (G) indicates significant difference as * P < 0.05, ** P < 0.01, and *** P < 0.001 by t-test. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz191, https://doi.org/10.1093/nar/gkz191 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 4. Functional redundancy of TZF1 and TZF4 in mediating Glc-TOR signaling. (A, B) The tzf1 tzf4 double mutant ... Figure 4. Functional redundancy of TZF1 and TZF4 in mediating Glc-TOR signaling. (A, B) The tzf1 tzf4 double mutant displays a modest but significant synergistic enhancement of Glc-TOR signaling response. Red arrows indicate the hypocotyl and root junctions. DAG, days after germination; D, day; Mock, sugar-free; Man, mannitol; Glc, glucose; Suc, sucrose; scale bars: 0.5 cm. Data represent mean ± s.e.m. of 16 plants. The result was shown from one of the three biological replicates with similar results. Root lengths were measured by using ImageJ. (C) DIC imaging of root meristem zones. Scale bar: 50 μm. The blue arrow indicates the root quiescent cells, and the red arrow indicates the transition between root meristem zone and elongation zone. (D) Quantitative analysis of the root meristem size in (C). Data represent mean ± s.e.m. of 16 plants. The result was shown from one of three independent experiments with similar results. The root meristem size was measured by using ImageJ. Different letters in (B) and (D) indicate significant differences at P < 0.05 by one-way ANOVA. Uppercase letters compared with each other in 15 mM Glc treatment condition, and lowercase letters compared with each other in 15 mM Suc treatment condition. (E) TOR expression level is significantly enhanced in tzf1 tzf4. The total RNA was isolated from the roots of 6-day-old seedlings grown in half-strength liquid MS medium without sugar in 12-h weak light (13 μmol m<sup>−1</sup> s<sup>−1</sup>) /12-h dark cycles at 22°C and harvested at ZT6. The gene expression level was normalized by the geometric mean of ACT2 and TUB4 expression. Data represent mean ± s.e.m. of three biological replicates. The asterisks indicate significant difference at P < 0.01 (**) by t-test. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz191, https://doi.org/10.1093/nar/gkz191 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 5. TZF1 acts downstream of PRR proteins to modulate TOR signaling. (A) The primary root length reduction of TZF1 ... Figure 5. TZF1 acts downstream of PRR proteins to modulate TOR signaling. (A) The primary root length reduction of TZF1 OE prr579 was not additive of prr579 and TZF1 OE upon activation of TOR signaling by sugars. Three-day-old seedlings grown in liquid sugar-free 1/2 MS were treated with 15 mM Glc or Suc for 3 days in weak light (22°C, LD). (Scale bar: 0.5 cm). (B) Quantitative analysis of the primary root length in (A). Red arrows indicate the hypocotyl and root junctions. Data represent mean ± s.e.m. of 16 plants. The result was shown from one of three independent experiments with similar results. Root lengths were measured by using ImageJ. (C) DIC imaging of root meristem zones. Scale bar: 50 μm. The blue arrow indicates the root quiescent cells, and the red arrow indicate the transition between meristem zone and elongation zone. (D) Quantitative analysis of the root meristem size in (C). Data represent mean ± s.e.m. of 15 plants. The result was shown from one of three independent experiments with similar results. The root meristem size was measured by using ImageJ. Different letters in (B) and (D) represent the significant difference as P < 0.05 by one-way ANOVA with SPSS software. Uppercase letters compare with each other in 15 mM Glc treatment condition, and lowercase letters compare with each other in 15 mM Suc treatment condition. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz191, https://doi.org/10.1093/nar/gkz191 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 6. TZF motif is critical for TZF1 to bind TOR mRNA and mediate TOR signaling. (A) The two intragenic mutations, ... Figure 6. TZF motif is critical for TZF1 to bind TOR mRNA and mediate TOR signaling. (A) The two intragenic mutations, G155E and H186Y in the transgene CaMV35S:TZF1-GFP abolished the TZF1 OE phenotypes. Compared to TZF1 OE plants, the two EMS point mutants (G155E in inter-ZF region and H186Y in 2<sup>nd</sup> zinc finger) displayed non-compact WT phenotypes. The expression levels of the two mutant GFP fusion proteins were comparable to that of the WT TZF1-GFP in a Western blot analysis detected by the GFP antibody. (B) TZF1 OE mutants (G155E and H186Y) were ineffective in triggering mRNA decay. Arabidopsis protoplasts were transiently co-expressed with a pair of effector (TZF1 WT or mutant protein) and reporter (GFP fused with ARE or mutated ARE (MutG) at 3′UTR) (see Lin et al., 2011 for detailed methods). WT TZF1 protein triggered GFP-ARE (but not GFP-MutG) reporter gene decay specifically, as indicated by reduced green fluorescence signals (upper left). No differences were observed in any effector-reporter pairs using G155E or H186Y mutant protein. Number in each image indicates the signal ratio of GFP/mCherry derived from the expression of reporter and effector, respectively. (C, D) The TZF1 (H186Y) OE and TZF1 (G155E) OE lines responded to Glc-TOR signaling normally. Three-day-old seedlings grown in liquid sugar-free 1/2 MS were treated with 15 mM Glc or Suc for 3 days in weak light (22°C, LD). Mock, sugar-free, Glc, glucose; Suc, sucrose. Data represent mean ± s.e.m. of 19 plants. The result was shown from one of three independent experiments with similar results. Root lengths were measured by using ImageJ. Scale bar in (C) is 0.5 cm. (E) Diagram depicting the putative TZF1-binding sites in the TOR. Black arrowheads indicate the positions of ARE elements, the putative TZF1-binding sites. Fragments underlined with numbers indicate the RIP-qPCR targets. (F) RIP–qPCR results indicated a direct binding of TZF1 to TOR mRNA. Ten-day-old seedlings grown at 22°C under LD condition was collected at ZT8 for this analysis. Data represent mean ± s.e.m. of three biological replicates. The asterisk indicates significant difference as ** P < 0.01 and *** P < 0.001 by t-test. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz191, https://doi.org/10.1093/nar/gkz191 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 7. Diminished TOR-signaling affects root architecture in TZF1 OE line and prr579 mutant. (A) Reduced lateral ... Figure 7. Diminished TOR-signaling affects root architecture in TZF1 OE line and prr579 mutant. (A) Reduced lateral root number in prr579 mutant and TZF1 OE line. The lower panel is a close-up view of the upper panel. The seedlings were grown on solid MS plates with 1% sucrose at 22°C under LD condition for 14 days. (B) Quantitative analysis of lateral root number per plant as shown in (A). Data represent mean ± s.e.m. of 15 plants. The result was shown from one of three independent experiments with similar results. The asterisk indicates significant difference as ** P < 0.01 by t-test. (C) Primary root elongation was reduced in both prr579 mutant and TZF1 OE line. The seedlings were grown on MS plates for 14 days in weak light (22°C, LD). Scale bar: 1 cm. Red arrows indicate the hypocotyl and root junctions. (D) Quantitative analysis of primary root length. The root lengths were measured by using ImageJ. Mock, sugar-free; Man, mannitol; Glc, glucose; Suc, sucrose. Data represent mean ± s.e.m. of 15 plants. The result was shown from one of three independent experiments with similar results. Different letters represent significant difference as P < 0.05 by one-way ANOVA with SPSS software. Uppercase letters compare with each other in 15 mM Glc treatment condition, and lowercase letters compare with each other in 15 mM Suc treatment condition. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz191, https://doi.org/10.1093/nar/gkz191 The content of this slide may be subject to copyright: please see the slide notes for details.

Figure 8. Proposed model of a novel plant circadian clock output mediating TOR signaling through PRRs-TZF1-TOR module. ... Figure 8. Proposed model of a novel plant circadian clock output mediating TOR signaling through PRRs-TZF1-TOR module. The underlying mechanism by which TOR feedback affects circadian clock remains to be determined. Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Nucleic Acids Res, gkz191, https://doi.org/10.1093/nar/gkz191 The content of this slide may be subject to copyright: please see the slide notes for details.