Differential interleukin-6 messenger ribonucleic acid expression and its distribution pattern in eutopic and ectopic endometrium Ali Salmassi, Ph.D.,

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Differential interleukin-6 messenger ribonucleic acid expression and its distribution pattern in eutopic and ectopic endometrium Ali Salmassi, Ph.D., Yahya Acil, Ph.D., Andreas Gerd Schmutzler, Dr.Med., Kerstin Koch, M.T.A., Walter Jonat, Dr.Med., Liselotte Mettler, Dr.Med. Fertility and Sterility Volume 89, Issue 5, Pages 1578-1584 (May 2008) DOI: 10.1016/j.fertnstert.2007.06.060 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 In vitro RT-PCR of IL-6. The lanes show the following: lane a, separation of a molecular weight marker; lanes b–d, IL-6 amplicon in human endometrium, endometriosis, and placenta as a positive control with primers P1 and P2; lanes e–g, IL-6 amplicon in human endometrium, endometriosis, and placenta as a positive control with primed in situ labeling primer P3 and P4; lane h, negative control, omitting RT; and lanes i–k, glyceraldehyde-3 phosphate dehydrogenase amplicon in human endometrium, endometriosis, and placenta tissue. Fertility and Sterility 2008 89, 1578-1584DOI: (10.1016/j.fertnstert.2007.06.060) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (a) Demonstration of IL-6 expression levels in endometriosis tissue (Es, n = 6), in endometrium (Em, n = 6), in placenta as positive control (C1–C3, dilutions of 1:5, 1:50, and 1:500), and in H2O as negative control (C4). (b) Comparison of relative IL-6 expression between endometriosis and non-endometriosis patients (each n = 6; P=.03, Wilcoxon test). Fertility and Sterility 2008 89, 1578-1584DOI: (10.1016/j.fertnstert.2007.06.060) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Detection of IL-6 by in situ RT-PCR technique. (a) Endometrium (magnification, ×200), (b) endometriosis (magnification, ×400), (c, d) tonsil and placenta as positive control (magnification, ×400), and (e) endometriotic tissue without primer as negative control (magnification, ×200). EP = epithelial cells; ST = stroma cells; PC = plasma cells; LC = lymphocytes; SC = syncytiotrophoblasts; and HC = fetal Hofbauer cells. Fertility and Sterility 2008 89, 1578-1584DOI: (10.1016/j.fertnstert.2007.06.060) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions