Prokineticin 1, homeobox A10, and progesterone receptor messenger ribonucleic acid expression in primary cultures of endometrial stromal cells isolated from endometrium of healthy women and from eutopic endometrium of women with endometriosis  Federica Tiberi, B.S., Anna Tropea, M.D., Federica Romani, M.D., Rosanna Apa, M.D., Riccardo Marana, M.D., Antonio Lanzone, M.D.  Fertility and Sterility  Volume 94, Issue 7, Pages 2558-2563 (December 2010) DOI: 10.1016/j.fertnstert.2010.03.006 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Prokineticin 1 (A) and PR mRNA (B) expression quantification obtained by real-time PCR according to the comparative cycle time (Ct) method in ESC isolated from control women after 72 and 96 hours of treatment with medium alone (CTR) or with a physiologic dose of MAP (5 × 10–8 M) alone (MAP) or in combination with E2 (4 × 10−10 M) (MAP + E2). The columns represent the mean ± SD of 2–ΔΔCt calculus from six different experiments. Glyceraldehyde-3-phosphate dehydrogenase and CTR sample were used as normalizer. Relative expression of each sample is expressed with respect to CTR sample (set equal to 1). Paired Student's t test was used to compare the average mRNA expression level between groups. A: ***P<.001 vs. CTR; °°°P<.001 vs. MAP. B: ***P<.001 vs. CTR; **P<.01 vs. CTR; °P<.05 vs. MAP. Fertility and Sterility 2010 94, 2558-2563DOI: (10.1016/j.fertnstert.2010.03.006) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Prokineticin 1 mRNA expression quantification obtained by real-time PCR according to the comparative cycle time (Ct) method in ESC isolated from control women and from women affected by endometriosis after 16 days of treatment with medium alone (CTR) or with MAP (2 × 10–7 M) and E2 (10−8 M) (MAP/E2). The columns represent the mean ± SD of 2–ΔΔCt calculus from six different experiments. Glyceraldehyde-3-phosphate dehydrogenase and CTR sample were used as normalizer. Relative expression of each sample is expressed with respect to CTR sample (set equal to 1). Paired Student's t test was used to compare the average mRNA expression level between groups. ***P<.001 vs. CTR. Fertility and Sterility 2010 94, 2558-2563DOI: (10.1016/j.fertnstert.2010.03.006) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Progesterone receptor (A) and HOXA10 (B) mRNA expression quantification obtained by real-time PCR according to the comparative cycle time (Ct) method in ESC isolated from control women and from women affected by endometriosis after 16 days of treatment with medium alone (CTR) or with MAP (2 × 10−7 M) and E2 (10−8 M) (MAP/E2). The columns represent the mean ± SD of 2–ΔΔCt calculus from six different experiments. Glyceraldehyde-3-phosphate dehydrogenase and CTR sample were used as normalizer. Relative expression of each sample is expressed with respect to CTR sample (set equal to 1). Paired Student's t test was used to compare the average mRNA expression level between groups. ***P<.001 vs. CTR. Fertility and Sterility 2010 94, 2558-2563DOI: (10.1016/j.fertnstert.2010.03.006) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions