Phylogeny of VexL pectate lyases. Phylogeny of VexL pectate lyases. The figure depicts a phylogram of VexL homologs, identified by position-specific iterative BLAST (19) of VexL from Achromobacter denitrificans. The maximum-likelihood phylogram was generated from 100 bootstrapped datasets using PhyML3.0 (64) and visualized using iTOL (65). GenBank (84) accession numbers for VexL homologs are listed in SI Appendix, Materials and Methods. Bootstraps are labeled as purple circles. Sean D. Liston et al. PNAS 2018;115:21:E4870-E4879 ©2018 by National Academy of Sciences