Proplatelets and large protrusions are morphologically distinct and possess different MT arrangements. Proplatelets and large protrusions are morphologically.

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Presentation transcript:

Proplatelets and large protrusions are morphologically distinct and possess different MT arrangements. Proplatelets and large protrusions are morphologically distinct and possess different MT arrangements. (A) Selected images from time-lapse DIC microscopy of proplatelet formation in vitro. One proplatelet tip has been followed (arrowhead). (B) Model constructed from TEM section of the same megakaryocyte (green). (C) MT (red) arrangement within the proplatelet tip. (D) MTs at the leading edge of the proplatelet. MTs within 50 nm of the plasma membrane are colored blue. (E) Model of large protrusion constructed from serial TEM sections showing the megakaryocyte (green) protrusion entering the sinusoid (pink). Free proplatelet-like structures (blue) are also present. (F) MT (red) arrangement within the protrusion tip. (G) MTs at the leading edge of the protrusion. MTs within 50 nm of the plasma membrane are colored blue. Scale bars represent 50 μm in (A), 10 μm in (B, E), and 1 μm in (C, D, F, G). Bone marrow of the diaphysis region obtained from flushed femurs. Images representative of three independent experiments. Source data are available for this figure. Edward Brown et al. LSA 2018;1:e201800061 © 2018 Brown et al.