Staurosporine-induced filamentation requires Cyr1 and PKA

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Staurosporine-induced filamentation requires Cyr1 and PKA Staurosporine-induced filamentation requires Cyr1 and PKA. (A) Farnesol inhibits filamentation induced by staurosporine. Staurosporine-induced filamentation requires Cyr1 and PKA. (A) Farnesol inhibits filamentation induced by staurosporine. SN95 wild-type cells were grown to log phase at 30°C in YPD or YPD plus 0.5 μg/ml staurosporine in the absence or presence of 200 μM farnesol. Cells were imaged by DIC microscopy. The scale bar indicates 10 μm. (B) Cyr1 and PKA are the only components of the cAMP signaling pathway tested that are required for filamentation induced by staurosporine. A CAI4 wild-type strain or mutants lacking components of the cAMP signaling pathway were subcultured to stationary phase in YPD at 30°C in the presence or absence of 0.5 μg/ml staurosporine (STS). Cells were imaged by DIC microscopy. The scale bar indicates 20 μm. Jinglin L. Xie et al. mSphere 2017; doi:10.1128/mSphere.00056-17