Erratum Experimental Hematology   Experimental Hematology  Volume 31, Issue 11, Pages 1135-1138 (November 2003) DOI: 10.1016/j.exphem.2003.09.019

Figure 1 Identification of a GFP+ cell in a slice of BM. (a): Representative photograph of a GFP+ cell directly observed in a longitudinally sliced femur of KSL3 (×50). GFP+ cells could be identified at the single cell level (arrowhead). (b): Immunohistostaining of a BM section on KSL10 using anti-GFP antibody secondary conjugated alkaline phosphatase (red, arrow). GFP+ cells were detected in the endosteal and mainly in the epiphysis region. These GFP+ cells were confirmed to be CD45+. (c): Representative photograph of the epiphysis region of the femur on KSL5, where most of GFP+ cells were identified (arrows). Bar: 1 mm. (d): Representative photograph of a diaphysis region of the femur on KSL5. No GFP+ cells were detected. Bar: 1 mm. Experimental Hematology 2003 31, 1135-1138DOI: (10.1016/j.exphem.2003.09.019)

Figure 2 Sequential observation of the GFP+ region in the femur of adult irradiated recipient mice. GFP+ regions (arrows or arrowheads) of femurs transplanted with wBM (a–c), Lin− (d–f), and KSL cells (g–i) are shown sequentially, on days 3, 10, and 20. (a, d, g: ×100; b, c, e, f, h, i: ×12). a, b, and c: High-power images of the epiphysis of a femur. These photographs are representative data from at least 9 transplanted mice for each day. Experimental Hematology 2003 31, 1135-1138DOI: (10.1016/j.exphem.2003.09.019)

Figure 3 Engraftment site in the femur after transplantation of KSL cells into adult irradiated mice. The initial engraftment site was analyzed 10 days after transplantation. The femur was categorized into two parts, the epiphysis and diaphysis regions, as described under Materials and methods. A and B are representative photographs of an engraftment site, one in the epiphysis and the other in diaphysis (×12 arrowhead). The distribution of engraftment sites where GFP+ cells appeared first was evaluated in irradiated adult recipient and classified into three groups. C shows the number of bones showing the first engraftment site, in the epiphysis, the diaphysis, or both regions (n=26). White column is for KSL cell transplantation and black column for Lin− cell transplantation. D and E show the extent and intensity of the GFP signal corresponding to the engraftment region in irradiated adult mice. Extent (D) and intensity (E) of GFP+ region in the femur (n=7) of irradiated adult mice 10 days after transplantation of KSL cells were calculated with Image-Pro Plus as described under Materials and methods. The GFP+ region in the epiphysis was significantly larger and brighter than that in the diaphysis region. Experimental Hematology 2003 31, 1135-1138DOI: (10.1016/j.exphem.2003.09.019)

Figure 4 Distribution of transplanted GFP+ cells in ribs and vertebrae. On days 3, 10, and 20 after transplantation, all bones were examined under a fluorescent stereomicroscope. The GFP+ regions (arrowheads) among ribs transplanted with wBM (a–c). Lin− (d–f), and KSL (g–i) cells are shown (a, d, g: ×100; b, c, e, f, h, i: ×12). The GFP+ regions were visible at intervals among the ribs, which were used as stepping-stones. The bottom panels (j, k: ×100) show the appearance of vertebrae after KSL cell transplantation. The white line indicates the shape of the vertebrae. These photographs are representative of at least 9 mice for each day after transplantation. Experimental Hematology 2003 31, 1135-1138DOI: (10.1016/j.exphem.2003.09.019)

Figure 5 Distribution of transplanted GFP+ cells in the spleen (A) and the thymus (B). Appearances of the spleen on days 3, 10, and 20 after transplantation with wBM (A, panels a, b, c), Lin− (A, panels a, b, c), and KSL (A, panels g, h) cells and appearances of the thymus on days 10, 20, and 30 after transplantation with KSL cells (B, panels a, b, c) are shown. GFP+ colonies were detected in the spleen (A, panels e, f, g, h), but the GFP+ region in the thymus was restricted throughout the experiment (B, panels a, b, c). Results shown here are representative of total of 48 mice examined. Experimental Hematology 2003 31, 1135-1138DOI: (10.1016/j.exphem.2003.09.019)

Figure 6 Sequential analysis of chimeric ratios of organs in irradiated adult mice. Chimeric ratios of BM (A), spleen (B), thymus (C), and peripheral blood (D) are shown on days 10, 20, and 30 after each type of transplantation. Black squares: wBM cell transplantation; black circle: Lin− cell transplantation; white circle: KSL cell transplantation. At least 3 mice were examined on each day and for each type of transplantation. Experimental Hematology 2003 31, 1135-1138DOI: (10.1016/j.exphem.2003.09.019)

Figure 7 Sequential observations of GFP+ regions in nonirradiated W/Wv mice. (A): GFP+ regions of the femur (panels a–d), ribs (panels e–g), and vertebrae (panels h–k) of W/Wv mice for transplantation of KSL cells are shown sequentially (×100). B shows the chimeric ratio corresponding to the appearance on KSL30. The upper panels show the appearance of each GFP+ region (a: epiphysis region of the femur, b: ribs, c: vertebrae [×100]). The lower panels show the chimeric ratios corresponding to each region. At least 9 mice were examined on each day for each type of transplantation. Extent (C) and fluorescence intensity (D) of the GFP+ region in femurs of nonirradiated W/Wv mice 4 months after transplantation of KSL cells were calculated with Image-Pro Plus as described under Materials and methods. C shows the ratio of the GFP+ area to the whole area of the femur in the epiphysis and diaphysis regions and D shows the accumulated fluorescence intensity in these regions. Experimental Hematology 2003 31, 1135-1138DOI: (10.1016/j.exphem.2003.09.019)