Composition of commercial media used for human embryo culture Dean E. Morbeck, Ph.D., Rebecca L. Krisher, Ph.D., Jason R. Herrick, Ph.D., Nikola A. Baumann, Ph.D., Dietrich Matern, M.D., Ph.D., Thomas Moyer, Ph.D.  Fertility and Sterility  Volume 102, Issue 3, Pages 759-766.e9 (September 2014) DOI: 10.1016/j.fertnstert.2014.05.043 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Development of individually cultured one-cell mouse embryos to the expanded blastocyst stage at 96 hours in commercially available culture media. Analysis of (A) all experimental treatments combined (with and without protein at 5% and 20% oxygen, n = 120–132 embryos per media), (B) comparison of 5% vs. 20% oxygen (n = 60–66 embryos per media), and (C) comparison of culture with or without protein (n = 60–66 embryos per media). Values with different letters are statistically different, P<.05. Fertility and Sterility 2014 102, 759-766.e9DOI: (10.1016/j.fertnstert.2014.05.043) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 (A) Timing of development from thawing to blastulation for one-cell mouse embryos (n = 30–33 per media) cultured without protein at 5% oxygen. Values represent mean ± SE in hours. a, IVC > Global (P<.05). b, IVC > Global and Vitrolife (P<.001). (B) Timing of development from thawing to blastulation for one-cell mouse embryos (n = 30–33 per media) cultured without protein at 20% oxygen. a, Global > Vitrolife (P<.05). b, Global > Sage and Vitrolife (P<.01). c, Sage > Vitrolife (P<.01). Fertility and Sterility 2014 102, 759-766.e9DOI: (10.1016/j.fertnstert.2014.05.043) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions