Evaluation of clonal relatedness of extended-spectrum β-lactamase-producing Proteus mirabilis isolates by quantitative antibiogram and RAPD typing Marie-Hélène.

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Evaluation of clonal relatedness of extended-spectrum β-lactamase-producing Proteus mirabilis isolates by quantitative antibiogram and RAPD typing Marie-Hélène Nicolas-Chanoine, Florence Espinasse-Maes Clinical Microbiology and Infection Volume 3, Issue 1, Pages 45-52 (February 1997) DOI: 10.1111/j.1469-0691.1997.tb00250.x Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Dendogram of 40 ESβla-producing Proteus mirabilis isolates obtained by quantitative antibiogram typing and their RAPD fingerprints. The hospitals where these strains were isolated are indicated in the right-hand lane. Clinical Microbiology and Infection 1997 3, 45-52DOI: (10.1111/j.1469-0691.1997.tb00250.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 RAPD fingerprints obtained with the primer HLWL74 of plasmid-free E. coli and isogenic E. coli derivatives harboring the plasmids encoding the nine representative transferable resistance patterns. Lane 1: E. coli J53-2. Lanes 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11: E. coli derived from P. mirabilis 18, 19, s3, 15, 16, 1, 7, 13, 17 and 30 respectively. Lane 12: E. coli MC-1061. Lane 13: E. coli derived from P. mirabilis 14. Lane 14: negative control. M′, molecular weight marker. Molecular sizes are indicated in kb on the left. Clinical Microbiology and Infection 1997 3, 45-52DOI: (10.1111/j.1469-0691.1997.tb00250.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 RAPD fingerprints of 40 ESβla-producing P. mirabilis strains obtained with the primer MHN1. The strain number is indicated above. M and M′, molecular weight markers. Molecular sizes are indicated in kb on the left. T–, negative control. The same fingerprint was obtained with P. mirabilis 1, 2, 3, 4, 5, 6, 7, 8, 17, 29, s1, s2, s3, s4 and s5. Another fingerprint was shared by P. mirabilis 91, 92, 95 and 15, and another by P. mirabilis 89, 90, 18, 19, 20, 21, 22, 31, 32, 33, 35, 36, 44 and 45. Strains 16, 30, 93, 15, 14, 34 and 13 each displayed a specific fingerprint. Clinical Microbiology and Infection 1997 3, 45-52DOI: (10.1111/j.1469-0691.1997.tb00250.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions