Volume 14, Issue 1, Pages (January 2016)

Slides:



Advertisements
Similar presentations
Volume 101, Issue 7, Pages (October 2011)
Advertisements

Binding Site in Eag Voltage Sensor Accommodates a Variety of Ions and is Accessible in Closed Channel  William R. Silverman, John P.A. Bannister, Diane.
Volume 95, Issue 11, Pages (December 2008)
Volume 14, Issue 4, Pages (February 2016)
The Mechanism of Na+/K+ Selectivity in Mammalian Voltage-Gated Sodium Channels Based on Molecular Dynamics Simulation  Mengdie Xia, Huihui Liu, Yang Li,
Structural Effects of an LQT-3 Mutation on Heart Na+ Channel Gating
Sebastian Meyer, Raimund Dutzler  Structure 
Volume 102, Issue 8, Pages (April 2012)
Volume 20, Issue 8, Pages (August 2012)
The Structure of the Cytoplasmic Domain of the Chloride Channel ClC-Ka Reveals a Conserved Interaction Interface  Sandra Markovic, Raimund Dutzler  Structure 
Chimeras Reveal a Single Lipid-Interface Residue that Controls MscL Channel Kinetics as well as Mechanosensitivity  Li-Min Yang, Dalian Zhong, Paul Blount 
Kimberly Matulef, Galen E Flynn, William N Zagotta  Neuron 
Volume 47, Issue 6, Pages (September 2005)
Volume 103, Issue 1, Pages (July 2012)
Brv1 Is Required for Drosophila Larvae to Sense Gentle Touch
Frank J. Smith, Victor P.T. Pau, Gino Cingolani, Brad S. Rothberg 
Large-Scale Conformational Dynamics of the HIV-1 Integrase Core Domain and Its Catalytic Loop Mutants  Matthew C. Lee, Jinxia Deng, James M. Briggs, Yong.
Crystal Structure of Tetrameric Arabidopsis MYC2 Reveals the Mechanism of Enhanced Interaction with DNA  Teng-fei Lian, Yong-ping Xu, Lan-fen Li, Xiao-Dong.
Volume 14, Issue 3, Pages (January 2016)
Volume 95, Issue 10, Pages (November 2008)
Narae Shin, Heun Soh, Sunghoe Chang, Do Han Kim, Chul-Seung Park 
Crystal Structures of a Ligand-free MthK Gating Ring: Insights into the Ligand Gating Mechanism of K+ Channels  Sheng Ye, Yang Li, Liping Chen, Youxing.
Amanda H. Lewis, Alisa F. Cui, Malcolm F. McDonald, Jörg Grandl 
Christoph Randak, Michael J Welsh  Cell 
Volume 106, Issue 6, Pages (March 2014)
Volume 5, Issue 5, Pages (December 2013)
Volume 20, Issue 12, Pages (December 2012)
A Gating Mechanism of the Serotonin 5-HT3 Receptor
Stationary Gating of GluN1/GluN2B Receptors in Intact Membrane Patches
Impaired Ca2+-Dependent Activation of Large-Conductance Ca2+-Activated K+ Channels in the Coronary Artery Smooth Muscle Cells of Zucker Diabetic Fatty.
Computational Modeling Reveals that Signaling Lipids Modulate the Orientation of K- Ras4A at the Membrane Reflecting Protein Topology  Zhen-Lu Li, Matthias.
Volume 7, Issue 7, Pages (July 2000)
Volume 76, Issue 3, Pages (November 2012)
Volume 20, Issue 7, Pages (July 2012)
Volume 15, Issue 6, Pages (June 2007)
Volume 23, Issue 6, Pages (June 2015)
Gating of HCN Channels by Cyclic Nucleotides: Residue Contacts that Underlie Ligand Binding, Selectivity, and Efficacy  Lei Zhou, Steven A. Siegelbaum 
The I182 Region of Kir6.2 Is Closely Associated with Ligand Binding in KATP Channel Inhibition by ATP  Lehong Li, Jing Wang, Peter Drain  Biophysical.
Volume 95, Issue 5, Pages (November 1998)
Volume 66, Issue 6, Pages (June 2010)
Volume 97, Issue 1, Pages (July 2009)
Volume 111, Issue 5, Pages (September 2016)
Extrapore Residues of the S5-S6 Loop of Domain 2 of the Voltage-Gated Skeletal Muscle Sodium Channel (rSkM1) Contribute to the μ-Conotoxin GIIIA Binding.
Energetics of Pore Opening in a Voltage-Gated K+ Channel
Mechanisms Contributing to T Cell Receptor Signaling and Assembly Revealed by the Solution Structure of an Ectodomain Fragment of the CD3ϵγ Heterodimer 
Fredrik Elinder, Michael Madeja, Hugo Zeberg, Peter Århem 
Localization of Divalent Cation-Binding Site in the Pore of a Small Conductance Ca2+- Activated K+ Channel and Its Role in Determining Current-Voltage.
Volume 101, Issue 7, Pages (October 2011)
Elementary Functional Properties of Single HCN2 Channels
Structure of the Staphylococcus aureus AgrA LytTR Domain Bound to DNA Reveals a Beta Fold with an Unusual Mode of Binding  David J. Sidote, Christopher.
A Point Mutation in Domain 4-Segment 6 of the Skeletal Muscle Sodium Channel Produces an Atypical Inactivation State  John P. O’Reilly, Sho-Ya Wang, Ging.
The Pore of the Voltage-Gated Proton Channel
Volume 7, Issue 7, Pages (July 2000)
Mechanism of Anionic Conduction across ClC
Volume 25, Issue 8, Pages e3 (August 2017)
Volume 23, Issue 4, Pages (April 2015)
Structural and Mechanistic Analysis of the Slx1-Slx4 Endonuclease
Volume 19, Issue 8, Pages (August 2011)
Pamela M. England, Yinong Zhang, Dennis A. Dougherty, Henry A. Lester 
Volume 25, Issue 9, Pages e3 (September 2017)
Volume 25, Issue 3, Pages (March 2000)
Parul Mishra, Julia M. Flynn, Tyler N. Starr, Daniel N.A. Bolon 
Volume 9, Issue 5, Pages (December 2014)
Volume 95, Issue 10, Pages (November 2008)
A Distinct Contribution of the δ Subunit to Acetylcholine Receptor Channel Activation Revealed by Mutations of the M2 Segment  Jian Chen, Anthony Auerbach 
Galen E Flynn, William N Zagotta  Neuron 
Dian Ding, Mengmeng Wang, Jing-Xiang Wu, Yunlu Kang, Lei Chen 
Structure of GABARAP in Two Conformations
Stimulatory Action of Internal Protons on Slo1 BK Channels
Volume 98, Issue 3, Pages (February 2010)
Presentation transcript:

Volume 14, Issue 1, Pages 129-139 (January 2016) Epilepsy-Related Slack Channel Mutants Lead to Channel Over-Activity by Two Different Mechanisms  Qiong-Yao Tang, Fei-Fei Zhang, Jie Xu, Ran Wang, Jian Chen, Diomedes E. Logothetis, Zhe Zhang  Cell Reports  Volume 14, Issue 1, Pages 129-139 (January 2016) DOI: 10.1016/j.celrep.2015.12.019 Copyright © 2016 The Authors Terms and Conditions

Cell Reports 2016 14, 129-139DOI: (10.1016/j.celrep.2015.12.019) Copyright © 2016 The Authors Terms and Conditions

Figure 1 Spatial Distribution and Conservation of the Epilepsy-Related Amino Acid Residues of the Slack Channel (A) Schematic representation of the Slack channel. The N and C termini are localized inside the cell. Four subunits together form the pore in the plasma membrane of the cell. The long C terminus includes two RCK domains (RCK, regulators of conductance of K+). The positions of epilepsy-related mutants are indicated in red on the schematic. (B) The Slack channel homology model structure used a template that the Kv1.2/Kv2.1 pore domain (green) is superimposed on the Slo1 channel C terminus (gray but with AC region highlighted in orange, the N terminus of the RCK1 domain containing 76 amino acids, a region including the secondary structures βA-αC named as AC region) (left, side view). The structure of gating ring of the Slack channel is shown without the pore domain (right, bottom view). (C) Sequence alignment for the context region of epilepsy-related Slack channel mutants. The amino acid residues related to epilepsy are shown in red. Conservative residues in the indicated species are shown (C1) V252, G269; (C2) R379, R409, and R455; (C3) I739 and Y775; and (C4) M875, R907, F911, A913, and A945. Human Slack (hslack) Genbank: 57582, Rat Slack (rslack) Genbank: 60444, Mouse Slack Genbank: 227632, Dog Slack (dslack) Genbank: 491258, Bovine Slack Genbank: 529468, Chicken Slack (cslack) Genbank: 395248, Zebrafish Slack (zslack) Genbank: 100004419. Conserved amino residues are shown with yellow background. Non-conserved residues are shown with cyan background. Cell Reports 2016 14, 129-139DOI: (10.1016/j.celrep.2015.12.019) Copyright © 2016 The Authors Terms and Conditions

Figure 2 Seven Epilepsy-Related Mutants Enhance Sodium Sensitivity of Slack Channel (A–C) Macroscopic typical current traces recorded from inside-out patches expressing WT (A), A945T (B), and Y775H mutants (C), respectively. Currents were elicited in 0–500 mM [Na+]i by a ramp protocol from −100 mV to +100 mV. (D and E) Sample Hill equation fits of the Na+ dose-response for macroscopic current data of WT (kd of 68 ± 6 mM, co-efficient number: n = 2.9) versus A945T (kd of 65 ± 3.6 mM, n = 3.3). (D) kd of 65 ± 3.6 mM, n = 3.3 and (E) WT versus Y775H (kd of 35 ± 3.4 mM, n = 3), respectively. (F) Summary of Na+ sensitivity of kd values for Slack mutants related to epilepsy. WT (kd of 68 ± 4.6 mM, n = 2.9), V252F (kd of 46 ± 4.7 mM, n = 3), G269S (kd of 49.2 ± 4.3 mM, n = 3.3), R379Q (kd of 79 ± 4.3 mM, n = 3.5), R409Q (kd of 57.5 ± 1.3 mM, n = 3.6), R455H (kd of 52 ± 3.8 mM, n = 2.7), I739M (kd of 63 ± 2 mM, n = 3), Y775H (kd of 35 ± 4.6 mM, n = 3.1), M875I (kd of 65.2 ± 2.9 mM, n = 3.2), R907C (kd of 49 ± 4.3 mM, n = 3.1), F911A (kd of 45.5 ± 1.9 mM, n = 3.0), A913T (kd of 59.5 ± 4.5 mM, n = 2.9), and A945T (kd of 65 ± 3.6 mM, n = 3.3). See also Figure S1. Cell Reports 2016 14, 129-139DOI: (10.1016/j.celrep.2015.12.019) Copyright © 2016 The Authors Terms and Conditions

Figure 3 Y775H and Y775D Mutants Are Directly Involved in Conferring Sodium Sensitivity to Slack Channels (A) Typical current traces recorded in the inside-out patch configuration for Y775D, the ramp protocol ran from −100 mV to +100 mV. (B) Hill equation fits of the Na+ dose–response data of the wild-type Slack channel, Y775H, Y775F, Y775E, and Y775D mutants. (C) Summary of kd values for the wild-type Slack (kd of 68 ± 6 mM, n = 2.9) and the Y775 mutants (Y775H kd of 35 ± 3.4 mM, n = 3.1; Y775D kd of 17.9 ± 1.3 mM, n = 3.2; Y775E kd of 48 ± 4 mM, n = 3.1; Y775F kd of 61 ± 3.3 mM, n = 2.9; Y775A kd of 48.2 ± 4 mM, n = 3.0; Y775R kd of 45 ± 2.4 mM, n = 3.1). ∗ indicates the mean values of groups are significantly different from the wild-type Slack channel. (D) Local structure of sodium binding site of wild-type Slack channel homology model. The Y775 (phenol ring in blue and other bond in cyan), sodium ion (yellow sphere), D818 and H823 (nitrogen ring in blue and other bond in cyan) belongs to the sodium coordination site of the wild-type Slack channel. In the wild-type channel, the Y775 residue is not positioned parallel to H823 residue and is not involved in sodium binding. (E) The local sodium sensing site structure of the Y775H mutant structure on the homology model. The H775 (nitrogen ring with N atom in yellow and the other bond in orange), sodium ion (yellow sphere), D818 (bond in orange and the carboxylate of D818 in red), and H823 (nitrogen ring with nitrogen atom in yellow and other bond in orange) form the sodium co-ordinate site of Y775H mutant. In the Y775H mutant, the sodium binds with D818 and is flanked by the H823 and H775 residues. (F) Local structure of the Slack channel Y775D mutant sodium binding site on the homology model. Cell Reports 2016 14, 129-139DOI: (10.1016/j.celrep.2015.12.019) Copyright © 2016 The Authors Terms and Conditions

Figure 4 Epilepsy-Related Mutants Localized on the RCK1 Domain Cause an Independent Conformational Change to Shift the Sodium Sensitivity of Slack Channels (A–C) Sample Hill equation fits of the Na+ dose-response macroscopic current data of WT and R409Q (kd of 57.5 ± 1.3 mM, n = 3.3), WT and R455H (kd of 52 ± 3.8 mM, n = 2.7), and WT and R379Q (kd of 79 ± 4.3 mM, n = 3), respectively. ★ indicates the mean values of groups are significantly different from the mean value of the wild-type Slack channel. (D) Typical macroscopic current traces recorded in the inside-out patch configuration for R409Q/R455H with [Na+]i from 0–200 mM. (E) Na+ dose-response data were fitted with the Hill equation for the wild-type Slack channel, R409Q/R455H mutant. (F) Summary of kd values for the wild-type Slack (WT kd of 68 ± 6 mM, n = 2.9, R409Q/R455H, kd of 18.4 ± 3 mM, n = 1.5, R409Q/R455H/R379Q, kd of 43.8 ± 3.4 mM, n = 3, R379Q/Y775H, kd of 49.6 ± 4.5 mM, n = 3.2) ○ indicates the mean values of groups are significantly different from the mean value of the wild-type Slack channel. ∗ indicates the mean values of groups are significantly different from the mean value of R455H/R409Q groups and R379Q groups. Cell Reports 2016 14, 129-139DOI: (10.1016/j.celrep.2015.12.019) Copyright © 2016 The Authors Terms and Conditions

Figure 5 Single-Channel Recordings Reveal that Epilepsy-Related Mutants Not Only Alter the Sodium Sensitivity but Also Enhance the Maximal Po of the Slack Channel (A–C) Typical single channel traces with [Na+]i from 50–200 mM of Y775H (A), R379Q (B), and R455H (C). (D and E) Sodium dose-dependent single-channel open probabilities (Po) of mutants are fitted by the Hill equation. The coefficient factors are indicated in the n value. (D) Slack WT: kd = 81 ± 3.7, n = 3.3; Pmax = 0.57 ± 0.02; R379Q: kd = 92 ± 5.4 mM, n = 4; Pmax = 0.81 ± 0.012, Y775H: kd = 38 ± 3.8 mM, n = 2.3; Pmax = 0.9 ± 0.027. (E) R455H: kd = 45 ± 2.5 mM, n = 4, R409Q: kd = 68 ± 0.7mM, n = 4. (F) Summary of kd values obtained from single channel recording by fitting Po of the epilepsy-related Slack channels mutants with the Hill equation. See also Figures S2, S3, S4, and S6 and Tables S1 and S2. Cell Reports 2016 14, 129-139DOI: (10.1016/j.celrep.2015.12.019) Copyright © 2016 The Authors Terms and Conditions

Figure 6 Double Mutant R409Q/R455H Not Only Enhances Channel Sodium Sensitivity but Also Leads to Channel Opening in the Absence of Na+ (A) Typical single-channel recording current traces in the inside-out patch configuration for R409Q/R455H with 0–200 mM [Na+]i. (B) The total amplitude histogram of R409Q/R455H in single-channel recording events in 0 mM [Na+]i were fitted by a Gaussian function. Each number in the y axis is times 10,000 events. (C) R409Q/R455H double mutant amplitude histogram with 100 mM [Na+]i were fitted with a Gaussian function. (D) Comparison of averaged Po values of WT Slack channel (black) with Po values of R409Q/R455H mutant (blue) in different concentrations of [Na+]i. Po of wild-type Slack channel is not measurable in 0 mM [Na+]i, Po = 0.15 ± 0.01 in 50 mM [Na+]i, Po = 0.31 ± 0.03 in 100 mM [Na+]i, Po = 0.43 ± 0.02 in 200 mM [Na+]i. R409Q/R455H (blue) Po = 0.013 ± 0.005 in 0 mM [Na+]i, Po = 0.45 ± 0.012 in 50 mM [Na+]i, Po = 0.74 ± 0.012 in 100 mM [Na+]i, Po = 0.75 ± 0.02 in 200 mM [Na+]i). See also Figure S6 and Tables S1 and S2. Cell Reports 2016 14, 129-139DOI: (10.1016/j.celrep.2015.12.019) Copyright © 2016 The Authors Terms and Conditions

Figure 7 Some Slack Channel Mutants Show Uniform Increases in Po at High Concentrations of [Na+]i Po of all mutants are summarized in increasing [Na+]i (A) to (C), respectively. (A) The Po of all mutants in 50 mM [Na+]i are shown. (B) The Po of all mutants in 100 mM [Na+]i are shown. (C) The Po of all mutants in 200 mM [Na+]i are shown. The percentage numbers of Po were summarized in Tables S1 and S2. See also Figures S5, S6, and S7. Cell Reports 2016 14, 129-139DOI: (10.1016/j.celrep.2015.12.019) Copyright © 2016 The Authors Terms and Conditions