Photon-Manipulated Drug Release from a Mesoporous Nanocontainer Controlled by Azobenzene-Modiﬁed Nucleic Acid † Molecular Science and Biomedicine Laboratory,

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Presentation transcript:

Photon-Manipulated Drug Release from a Mesoporous Nanocontainer Controlled by Azobenzene-Modiﬁed Nucleic Acid † Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Biology, and College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China, ‡ Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center and UF Genetics Institute, Center for Research at the Bio/Nano Interface, University of Florida, Gainesville, Florida 32611-7200, United States, § State Key Laboratory of Rare Earth Materials Chemistry and Applications, Peking University, Beijing 100871, China, and ^ College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072, China

Figure 2. (a) Synthetic strategy for arm-DNA grafting onto the surface of MSNs. (b) Representative SEM image of DNAfunctionalized MSN. (c) Small-angle XRD of MSNs, cyanatofunctioned MSN, and DNA-modiﬁed MSN. (d) TEM image of DNA-modiﬁed MSNs.

Figure 3. (a) Time course of Rh6G release from MSN modified by azobenzene- incorporated DNA (azo-DNA-4). Data have been normalized to the maximumlevel of dye released in the experiment. (b) UV-light-responsive release from MSN system modified with azobenzene-incorporated DNA (azo-DNA-4) and normal DNA for 1500 min, as determined by fluorescence intensity. Data have been normalized to the loading amount of dye in the experiment.

Figure S3. UV light responsive release of Rh6G toward MSN functionalized with different azobenzene incorporated DNA (azoDNA-1, azoDNA-2, azoDNA-3, and azoDNA-4) for 1500 min, as measured by fluorescence intensity.

In summary, we have demonstrated, as a proof-of- concept, how the incorporation of azobenzene into a novel biocompatible DNA/MSN molecular valve can impart controllable photo responsive release behaviour. (2) We exploited this azo-DNA/MSN nanoplatform as a carrier for the anticancer drug DOX because of its good biocompatibility via MTT assay. (3) The endocytosis of DOX-loaded azo-DNA/MSN by A549 cells was demonstrated through confocal laser microscopy.