Two chromosomally located qnrB variants, qnrB6 and the new qnrB16, in Citrobacter spp. isolates causing bacteraemia J. Sanchez-Cespedes, S. Marti, S.M.

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Two chromosomally located qnrB variants, qnrB6 and the new qnrB16, in Citrobacter spp. isolates causing bacteraemia J. Sanchez-Cespedes, S. Marti, S.M. Soto, V. Alba, C. Melción, M. Almela, F. Marco, J. Vila Clinical Microbiology and Infection Volume 15, Issue 12, Pages 1132-1138 (December 2009) DOI: 10.1111/j.1469-0691.2009.02744.x Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Pulsed-field gel electrophoresis of the three clinical Citrobacter freundii isolates. DNA was digested with XbaI. M, lambda marker; 1, C. freundii 72857; 2, C. freundii 62778; 3, C. freundii 21112. Clinical Microbiology and Infection 2009 15, 1132-1138DOI: (10.1111/j.1469-0691.2009.02744.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Plasmid identification in Klebsiella pneumoniae 46408 by genomic mapping with I-CeuI and digestion with SI nuclease. (a) Pulsed-field gel electrophoresis (PFGE). Lane M: lambda ladder PFGE marker. Lane 1: digestion with I-CeuI. Lane 2: digestion with SI nuclease. (b) Digestion with I-CeuI. Lane 1: hybridization with a 23S rRNA gene probe. Lane 2: hybridization with a qnrS probe. (c) Digestion with SI nuclease. Lane 1: hybridization with a 23S rRNA gene probe. Lane 2: hybridization with a qnrS probe. Arrows indicate plasmid location. Clinical Microbiology and Infection 2009 15, 1132-1138DOI: (10.1111/j.1469-0691.2009.02744.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Genomic localization of qnrB by genomic mapping with I-CeuI and digestion with S1 nuclease. (a) Pulsed-field gel electrophoresis (PFGE). A1, digestion with I-CeuI; A2, digestion with S1 nuclease. (b) Digestion with I-CeuI. B1, hybridization with a probe for the 23S rRNA gene; B2, hybridization with a qnrB probe. (c) Digestion with S1 nuclease. C1, hybridization with a 23S rRNA gene probe; C2, hybridization with a qnrB probe. Lane M: lambda ladder PFGE marker. Lane 1: Citrobacter freundii 72857. Lane 2: C. freundii 62778. Lane 3. C. freundii 21112. Lane 4: Citrobacter werkmanii 14.0. The rectangle in A1 covers the fragments shown in Fig. 4 after changing the PFGE conditions. The arrow represents the slowest-moving band of the lambda ladder (727.5 kb). Clinical Microbiology and Infection 2009 15, 1132-1138DOI: (10.1111/j.1469-0691.2009.02744.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 4 Localization of qnrB on I-CeuI-digested DNA following pulsed-field gel electrophoresis (PFGE) under the conditions described by Liu et al. [13] (a) PFGE. (b) Hybridization with a 23S rRNA gene probe. (c) Hybridization with a qnrB probe. Lane 1: Citrobacter freundii 72857. Lane 2: C. freundii 62778. Lane 3: C. freundii 21112. Lane 4: Citrobacter werkmanii 14.0. The arrow indicates the position of the slowest-moving band of the lambda ladder (727.5 kb). Clinical Microbiology and Infection 2009 15, 1132-1138DOI: (10.1111/j.1469-0691.2009.02744.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 5 Undigested DNA after pulsed-field gel electrophoresis (PFGE) under the conditions described by Liu et al. [13] (a) PFGE. (b) Hybridization with a qnrB probe. Lane 1: Citrobacter freundii 21112. Lane 2: Citrobacter werkmanii 14.0. The arrow indicates undigested qnrB-containing DNA. Clinical Microbiology and Infection 2009 15, 1132-1138DOI: (10.1111/j.1469-0691.2009.02744.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions