Gas Chromatography/Mass Spectrometry Characterization of Corticosteroid Metabolism in Human Immortalized Keratinocytes  Andrzej Slominski  Journal of Investigative Dermatology  Volume 118, Issue 2, Pages 310-315 (February 2002) DOI: 10.1046/j.0022-202x.2001.01648.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 PROG and DOC metabolism. TLC separation of products of PROG (P4) and DOC metabolism in cultured human keratinocytes was done using the following solvents: methanol 6%/chloroform/water 0.01% (MEOH: CHL); acetone 20%/chloroform (ACETONE: CHL); and tetrahydrofuran 50%/chloroform (THF:CHL). Journal of Investigative Dermatology 2002 118, 310-315DOI: (10.1046/j.0022-202x.2001.01648.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Mass spectra of the MOTMS derivatives of the steroids identified as (A) 3α,21-dihydroxy-5α-pregnan-20-one (3α,5α-THDOC) and (B) 3α,6,21-trihydroxy-5α-pregnan-20-one (6-hydroxyTHDOC). Insert: predicted structure of the compound. Journal of Investigative Dermatology 2002 118, 310-315DOI: (10.1046/j.0022-202x.2001.01648.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Mass spectra of the MOTMS derivatives of the steroids identified as (A) 3 β,21-dihydroxy-5-pregnen-20-one (3 βΔ5-21-OHpregnenolone), (B) 3α,21-dihydroxy-5-pregnen-20-one (3αΔ5-21-OHpregnenolone), and (C) standard (authentic) 3β,21-dihydroxy-5-pregnen-20-one. Insert: predicted structure of the compound. Journal of Investigative Dermatology 2002 118, 310-315DOI: (10.1046/j.0022-202x.2001.01648.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Proposed pathway for the metabolism of DOC in human keratinocytes. Journal of Investigative Dermatology 2002 118, 310-315DOI: (10.1046/j.0022-202x.2001.01648.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions