Volume 26, Issue 2, Pages e4 (February 2018)

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Volume 26, Issue 2, Pages 337-344.e4 (February 2018) MonoRes: Automatic and Accurate Estimation of Local Resolution for Electron Microscopy Maps Jose Luis Vilas, Josué Gómez-Blanco, Pablo Conesa, Roberto Melero, José Miguel de la Rosa-Trevín, Joaquin Otón, Jesús Cuenca, Roberto Marabini, José María Carazo, Javier Vargas, Carlos Oscar S. Sorzano Structure Volume 26, Issue 2, Pages 337-344.e4 (February 2018) DOI: 10.1016/j.str.2017.12.018 Copyright © 2018 Elsevier Ltd Terms and Conditions

Structure 2018 26, 337-344.e4DOI: (10.1016/j.str.2017.12.018) Copyright © 2018 Elsevier Ltd Terms and Conditions

Figure 1 Signal Decomposition From an Original Signal s(t) (continuous), the Hilbert Transform is calculated (dotted) and the Amplitude or Envelope of the Analytic Signal is shown (dashed). Structure 2018 26, 337-344.e4DOI: (10.1016/j.str.2017.12.018) Copyright © 2018 Elsevier Ltd Terms and Conditions

Figure 2 MonoRes Workflow Input volumes, the algorithm (middle box blocks), and the result (i.e., the resolution map - down block). HPF, high-pass filter; LPF, low-pass filter. Structure 2018 26, 337-344.e4DOI: (10.1016/j.str.2017.12.018) Copyright © 2018 Elsevier Ltd Terms and Conditions

Figure 3 Filtered Signals at different frequencies and their Monogenic Amplitudes Frequencies are shown on the left and amplitudes on the right. The monogenic amplitude of noise is indicated in green, signal or monogenic amplitude with added noise in blue, and without noise in red. Structure 2018 26, 337-344.e4DOI: (10.1016/j.str.2017.12.018) Copyright © 2018 Elsevier Ltd Terms and Conditions

Figure 4 MonoRes Results for Synthetic Maps (A) Glutamate dehydrogenase filtered at 2, 4, and 6 Å from the PDB: 5K12 atomic model. (B) The synthetic large map (1,000 × 1,000 × 1,000) from the PDB: 3JC9 filtered at 10 Å. For (A and B) MonoRes resolution map (left), resolution slices obtained by MonoRes (right). Structure 2018 26, 337-344.e4DOI: (10.1016/j.str.2017.12.018) Copyright © 2018 Elsevier Ltd Terms and Conditions

Figure 5 MonoRes Resolution Map, MonoRes and ResMap Resolution Slices, and Histograms for the Experimental Maps (A–C) (A) β-galactosidase (EMDB-2984), (B) proteosome (EMDB-6287) and (C) TRPV1 (EMDB: EMD-5778). Resolution maps are on the left, MonoRes and ResMap resolution slices are in the middle, and histograms on the right. Structure 2018 26, 337-344.e4DOI: (10.1016/j.str.2017.12.018) Copyright © 2018 Elsevier Ltd Terms and Conditions

Figure 6 Local Resolution Results MonoRes resolution map (left), and MonoRes, ResMap, and blocres resolution slices (center) and histograms (right) for the experimental half-maps of (A) β-galactosidase (EMDB: EMD-2984) and (B) proteosome (EMDB: EMD-6287). Structure 2018 26, 337-344.e4DOI: (10.1016/j.str.2017.12.018) Copyright © 2018 Elsevier Ltd Terms and Conditions

Figure 7 Local Filtering Results (A) Original volume (top) and slice (bottom) of the TRPV1 (EMDB: EMD-5778). (B) Volume locally filtered by its local resolution values. Structure 2018 26, 337-344.e4DOI: (10.1016/j.str.2017.12.018) Copyright © 2018 Elsevier Ltd Terms and Conditions

Figure 8 MonoRes Resolution Map, Resolution Slice, and Histogram Obtained for the Aquareovirus (EMDB: EMD-5160) Structure 2018 26, 337-344.e4DOI: (10.1016/j.str.2017.12.018) Copyright © 2018 Elsevier Ltd Terms and Conditions