High correlation of expression changes of NMD-regulated genes identified by both the pSILAC screen and previously reported global RNA screens after UPF1.

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High correlation of expression changes of NMD-regulated genes identified by both the pSILAC screen and previously reported global RNA screens after UPF1 depletion. High correlation of expression changes of NMD-regulated genes identified by both the pSILAC screen and previously reported global RNA screens after UPF1 depletion.A, Heatmap comparing the log2-fold change of expression of UPF1 targets on protein (pSILAC) or RNA level (9, 11, 12, 14). Only genes are shown that have been identified in the pSILAC screen and at least 2 RNA screens. B–E, Candidate genes that have been identified by our pSILAC approach and by 3 out of 4 independent RNA screens show a high correlation of expression regulation. R = Pearson correlation coefficient. Jana Sieber et al. Mol Cell Proteomics 2016;15:1584-1597 © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.