Do the matrix degrading enzymes cathepsins B and D increase following a high intensity exercise regime? E.A. Bowe, Ph.D., R.C. Murray, Ph.D., L.B. Jeffcott,

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Presentation transcript:

Do the matrix degrading enzymes cathepsins B and D increase following a high intensity exercise regime? E.A. Bowe, Ph.D., R.C. Murray, Ph.D., L.B. Jeffcott, Ph.D., M.E. Davies, Ph.D. Osteoarthritis and Cartilage Volume 15, Issue 3, Pages 343-349 (March 2007) DOI: 10.1016/j.joca.2006.08.014 Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 (A) Diagram of sites from which the cartilage was taken in the MCJ of the left forelimb. For the diagram of the entire limb, dorsal is to the left of the diagram and palmar is to the right. For the diagram of the carpal bones, medial is to the left and lateral to the right (RP; RD; IP; ID, modified from Murray et al.25). (B) Toluidine Blue staining of equine cartilage defining the cartilage zones from which the cell counts were taken: the AS contains flattened cells near the surface; the MZ contains rounder cells that are individually spaced; the DZ contains larger cells, clumped together. Osteoarthritis and Cartilage 2007 15, 343-349DOI: (10.1016/j.joca.2006.08.014) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Typical immunolocalisation of cathepsin B (A) within articular cartilage from a high intensity exercised horse (bar=600μm). (B) Magnification of box in A (bar=300μm). (C) An intense staining of cathepsin B was noted in chondrocyte clusters (arrow head), adjacent to the damaged AS (asterisk) (bar=100μm). Osteoarthritis and Cartilage 2007 15, 343-349DOI: (10.1016/j.joca.2006.08.014) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 The percentage of cathepsin B positive cells at each test site in low and high intensity exercise groups within the (A) AS, (B) MZ, (C) DZ. Grey bars, low intensity exercise; black bars, high intensity exercise. Osteoarthritis and Cartilage 2007 15, 343-349DOI: (10.1016/j.joca.2006.08.014) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 (A) The percentage of cathepsin B positive cells throughout the cartilage depth at the four test sites (RD; RP; ID; IP). (B) The percentage of cathepsin B positive cells within each cartilage zone, pooled from all dissection sites (AS; MZ; DZ). Grey bars, low intensity exercise; black bars, high intensity exercise; *, P<0.001. Osteoarthritis and Cartilage 2007 15, 343-349DOI: (10.1016/j.joca.2006.08.014) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Typical immunolocalisation of cathepsin D (A) within articular cartilage from a high intensity exercised horse (bar=600μm). (B) Magnification of box in A (bar=300μm). (C) An intense staining of cathepsin D was noted in chondrocyte clusters (arrow heads), adjacent to the damaged AS (asterisk) (bar=100μm). Osteoarthritis and Cartilage 2007 15, 343-349DOI: (10.1016/j.joca.2006.08.014) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 The percentage of cathepsin D positive cells at each test site in low and high intensity exercise groups within the (A) AS, (B) MZ, (C) DZ. Grey bars, low intensity exercise; black bars, high intensity exercise; #P<0.05; a, P<0.05 low intensity vs high intensity exercise. Osteoarthritis and Cartilage 2007 15, 343-349DOI: (10.1016/j.joca.2006.08.014) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 (A) The percentage of cathepsin D positive cells throughout the cartilage depth at the four test sites (RD; RP; ID; IP). (B) The percentage of cathepsin D positive cells within each cartilage zone, pooled from all dissection sites (AS; MZ; DZ). Grey bars, low intensity exercise; black bars, high intensity exercise; #P<0.05; ∗, P<0.01; a, P<0.05 low intensity vs high intensity exercise. Osteoarthritis and Cartilage 2007 15, 343-349DOI: (10.1016/j.joca.2006.08.014) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions