UV Induces p21WAF1/CIP1 Protein in Keratinocytes Without p53 Ming Liu, Norbert M. Wikonkal, Douglas E. Brash Journal of Investigative Dermatology Volume 113, Issue 2, Pages 283-284 (August 1999) DOI: 10.1046/j.1523-1747.1999.00657.x Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions
Figure 1 In vivo induction of p21WAF1/CIP1 protein by UV is p53-independent. Wild-type (p53+/+) and p53 knockout (p53−/−) mice (Ziegler et al. 1994) were mock-irradiated or irradiated with a UVB sun lamp for a dose of 1000 J per m2 at a dose rate of 5.5 J per m2 per s. This lamp (FS20T12-UVB, National Biological;Ziegler et al. 1994) emits wavelengths between 270 and 420 nm, with peak emission at 313 nm (UVB, 84.3%; UVA, 15.7%; UVC, 0.1%). Twenty-four hours later, mice were sacrificed. Skin flaps were formalin-fixed and paraffin-embedded. Five micometer sections were then stained with p21WAF1/CIP1 antibody (Ab 5, Oncogene Research Products) by the standard 3,3′-diaminobenzidine (DAB) immunohistochemistry procedure with antigen retrieval. Journal of Investigative Dermatology 1999 113, 283-284DOI: (10.1046/j.1523-1747.1999.00657.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions
Figure 2 p21WAF1/CIP1 protein and mRNA expression in p53-null NHK4 keratinocytes after UV irradiation. Western (upper panel) and northern (middle panel) blot analysis of NHK4 cells isolated 5 h and 8 h after exposure to 40 and 120 J per m2 of UV (same lamp as in Figure 1); ribosomal RNA loading control on ethidium bromide stained gel before transfer (lower panel). Journal of Investigative Dermatology 1999 113, 283-284DOI: (10.1046/j.1523-1747.1999.00657.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions