Mean feature profiles of targeted cells are highly consistent between gRNA sequences Mean feature profiles of targeted cells are highly consistent between.

Slides:



Advertisements
Similar presentations
Chapter 2 Exploring Data with Graphs and Numerical Summaries
Advertisements

5 Number Summary Box Plots. The five-number summary is the collection of The smallest value The first quartile (Q 1 or P 25 ) The median (M or Q 2 or.
Box and Whisker Plots and the 5 number summary Chapter 6 Section 7 Ms. Mayer Algebra 1.
BOX PLOTS/QUARTILES. QUARTILES: 3 points in a set of data that separate the set into 4 equal parts. Lower Quartile: Q1 (The median for the lower half.
Quantitative data. mean median mode range  average add all of the numbers and divide by the number of numbers you have  the middle number when the numbers.
Box and Whisker Plots and the 5 number summary Mr. J.D. Miles Turner Middle School Atlanta Georgia
Box and Whisker Plots and the 5 number summary Mr. J.D. Miles Turner Middle School Atlanta Georgia
Cumulative frequency Cumulative frequency graph
Box Plots March 20, th grade. What is a box plot? Box plots are used to represent data that is measured and divided into four equal parts. These.
What is a box-and-whisker plot? 5-number summary Quartile 1 st, 2 nd, and 3 rd quartiles Interquartile Range Outliers.
Box and Whisker Plots.
Box and Whisker Plots and the 5 number summary
Box and Whisker Plots and the 5 number summary
Tuning the response of the DPI
Abundance of proteins matching selected subcellular locations and functions in CaCo‐2 cells. Abundance of proteins matching selected subcellular locations.
Roles of stable RNA structures, 5ʹ TOP sequences, and sequence motifs within 5ʹUTR for translational regulation Roles of stable RNA structures, 5ʹ TOP.
MRNA coexpression of neighbouring genes is driven by chromatin fluctuations and regulatory interference mRNA coexpression of neighbouring genes is driven.
Overview of the experimental setting.
Melting behavior of protein complexes
Functional analysis of duplicate pair CIK1–VIK1 (A) Genetic interaction profile similarity. Functional analysis of duplicate pair CIK1–VIK1 (A) Genetic.
Validation of the CCE associations by mass spectrometry.
The absolute value of each deviation.
Comparative analysis of RNA and protein profiles.
Prediction of IGHV status based on Factor 1 in the CLL data and validation on outlier cases on independent assays Prediction of IGHV status based on Factor.
Disease‐causing mutations influence the association of CSPα with partner proteins Disease‐causing mutations influence the association of CSPα with partner.
Large‐scale image‐based CRISPR‐Cas9 gene perturbation profiling
Mean As A Balancing Point
Buffering of non‐functional mRNA co‐regulation likely is a passive process Buffering of non‐functional mRNA co‐regulation likely is a passive process Percentage.
Box and Whisker Plots.
Comparing histone profiles and DNA accessibility of secretory and enterocyte progenitor cells Comparing histone profiles and DNA accessibility of secretory.
Dynamics and variability of SMAD2 signaling in single cells
IRE1 signaling signatures in glioblastoma multiform
Measures of Central Tendency
Constructing Box Plots
Effect of mTOR inhibition on the Myc‐induced response
Heat maps showing global relative growth phenotype and comparison between measured and predicted values. Heat maps showing global relative growth phenotype.
Statistics and Data (Algebraic)
Differences in cellular organization emerge from quantitative proteomic experiments Differences in cellular organization emerge from quantitative proteomic.
Mean As A Balancing Point
RNAi causes widespread changes in cell population context leading to predictable changes in virus infection. RNAi causes widespread changes in cell population.
Examples for intrachromosomal mRNA co‐regulation patches
Collective shifts of abundance for nuclear and extracellular proteins in colorectal cancer Collective shifts of abundance for nuclear and extracellular.
Phenotypic profiling by between‐well comparison of feature profiles
Small‐molecule‐driven cell‐type enrichment as a model for intestinal differentiation Small‐molecule‐driven cell‐type enrichment as a model for intestinal.
Box and Whisker Plots and the 5 number summary
DeathPro dyes Hoechst and PI do not affect organoid growth and drug responses DeathPro dyes Hoechst and PI do not affect organoid growth and drug responses.
Evaluation of assay performance with positive and negative binary reference sets Evaluation of assay performance with positive and negative binary reference.
A. A. The two independent mutations identified in the structural gene of pqn-82 are shown above the gene. The genomic coordinates for the altered base.
Box-And-Whisker Plots
Two‐way unsupervised uncentered unnormalized hierarchical clustering using a Pearson's correlation of the phenotypic profiles of 4281 nonessential genes.
Comparison of nuclear surface area of HeLa and RKO cells
Box and Whisker Plots and the 5 number summary
Simulation showing that the cell length variability of the entire population can mask abnormal cell length variability at a specific cell cycle period.
Box and Whisker Plots.
Kinome‐wide activity regulation derived from known substrates and 41 quantitative phosphoproteomic studies Kinome‐wide activity regulation derived from.
An integrated NHR network.
Large‐scale image‐based CRISPR‐Cas9 gene perturbation profiling
Population kinetics and simulation of E2 induction. Related to Fig 4
Validation of phosphatase hits mapping prediction on the optimized growth model. Validation of phosphatase hits mapping prediction on the optimized growth.
Box and Whisker Plots and the 5 number summary
Examples of differentiating humoral response features identified by classification models Examples of differentiating humoral response features identified.
Warm-Up Define mean, median, mode, and range in your own words. Be ready to discuss.
Box and Whisker Plots and the 5 number summary
Drug sensitivity described by LD50 is similar in cells cultured in 2D or as organoids Drug sensitivity described by LD50 is similar in cells cultured in.
Gene signatures of wild‐type cells upon stimulation
Effect of rare SVs on cardiac transcription in DCM
Kinetics of GREB1 induction by estrogen in single cells (see also Fig EV4 and Movie EV3)‏ Kinetics of GREB1 induction by estrogen in single cells (see.
CRISPR‐Cas9 gene perturbation profiling in HeLa cells
BRI1 signaling at the dividing cells restores overall root growth
Presentation transcript:

Mean feature profiles of targeted cells are highly consistent between gRNA sequences Mean feature profiles of targeted cells are highly consistent between gRNA sequences Boxplots of the standardized single‐cell feature values of all transfected cells and phenotypically perturbed cells transfected with plasmids targeting NUP62, HSPA5, NUP107 or NUP98, bar graph representation of the percentage of T(+) cells with a PV > 0.62. Boxes indicate the 1st and 3rd quartile of the data distribution. The whiskers indicate the maximum and minimum datapoints within the 1st quartile minus 1.5 times the interquartile range (IQR) of the data and the third quartile plus 1.5 times the IQR.Schematic representation of the NPC, adapted from Weberruss and Antonin (Weberruss & Antonin, 2016).Cells were transfected with three independent plasmids targeting each of the genes NUP62, HSPA5, NUP107 or NUP98. Mean feature profiles were obtained from all transfected cells, or the subset of T(+) cells with a high PV. The Pearson correlation coefficient between pairs of profiles obtained from populations targeted for the same gene with different plasmids was calculated. The correlations between profiles obtained from all transfected cells, or the subset of T(+) cells with a high PV are compared.Boxplots of Pearson's correlation coefficients calculated between mean feature profiles of phenotypically perturbed cells transfected with plasmids targeting the same gene, or different genes. Phenotypic profiles were obtained from cells transfected with plasmids targeting selected subunits of the NPC and HSPA5 (green) or the top‐scoring genes that were identified in the large‐scale profiling of the mAb414 staining features for which multiple targeting plasmids were present in the library (grey). Boxes indicate the 1st and 3rd quartile of the data distribution. The whiskers indicate the maximum and minimum datapoints within the 1st quartile minus 1.5 times the interquartile range (IQR) of the data and the third quartile plus 1.5 times the IQR. Reinoud de Groot et al. Mol Syst Biol 2018;14:e8064 © as stated in the article, figure or figure legend