Lnp protein requires phospholipids to form stacked discs.

Slides:

Advertisements

Similar presentations

Fig. 4. Expressional profiles of apolipoproteins in HDL

Advertisements

Volume 27, Issue 1, Pages (October 2013)

Interaction of SRP19 with nuclear transport receptors.

Volume 93, Issue 6, Pages (June 1998)

BRCA1 Is Associated with a Human SWI/SNF-Related Complex

Opsin Is a Phospholipid Flippase

The coiled-coil domains of Lnp are required for bicelle stacking.

H-NS2 protein interacts with Lon and H-NS protein in vitro.

Lnp-mediated stacked bicelle formation requires the P domain.

Reconstituted human Lnp forms stacked discs.

H-NS2 is upregulated in an hns mutant derivative of the strain E

A A B C D Fraction number b MCF7 lysate fraction A B C D A B C D GST-p851 GST Kinase assay GST-p851.

Volume 94, Issue 1, Pages (July 1998)

Volume 3, Issue 5, Pages (May 2013)

Purification of budding yeast cohesin and its loader.

Last class Salting out Dialysis Paper 1 discussion.

Distinct Roles for CTD Ser-2 and Ser-5 Phosphorylation in the Recruitment and Allosteric Activation of Mammalian mRNA Capping Enzyme C.Kiong Ho, Stewart.

RNA Seeds Higher-Order Assembly of FUS Protein

Volume 15, Issue 2, Pages (July 2004)

Calcium induced CHGB aggregation and single particle analysis of CHGB dimers. Calcium induced CHGB aggregation and single particle analysis of CHGB dimers.

Transcription Factor MIZ-1 Is Regulated via Microtubule Association

Fig. 2. Binding of recombinant full-length Lm-411 to muscle receptors, myotubes, and self-polymerization is enhanced by mag and αLNNd. Binding of recombinant.

HMGN Proteins Act in Opposition to ATP-Dependent Chromatin Remodeling Factors to Restrict Nucleosome Mobility Barbara P. Rattner, Timur Yusufzai, James.

Effect of NTRs on NPCs. Effect of NTRs on NPCs. (A–D) Images from a video sequence (see Video 1) of the cytoplasmic side of the NE, in which, step-by-step,

Volume 38, Issue 5, Pages (June 2010)

Membrane insertion of CHGB introduces positive curvature in bilayers.

Volume 21, Issue 9, Pages (September 2013)

Volume 113, Issue 2, Pages (April 2003)

Kinesin 1 Drives Autolysosome Tubulation

Sichen Shao, Ramanujan S. Hegde Molecular Cell

Volume 28, Issue 6, Pages (December 2007)

2-D gel images visualized by Coomassie Brilliant Blue staining representing total proteins extracted from HCT-8 under apoptotic conditions in 2 mm Gln.

Enlargements of 2-D gels visualized by Coomassie Brilliant Blue staining. Enlargements of 2-D gels visualized by Coomassie Brilliant Blue staining. In.

Richard W. Deibler, Marc W. Kirschner Molecular Cell

Volume 108, Issue 1, Pages (January 2015)

In vitro sterol transport activity of WT and MR/ND mutant START domain of STARD3 In vitro sterol transport activity of WT and MR/ND mutant START domain.

Condensins, Chromosome Condensation Protein Complexes Containing XCAP-C, XCAP-E and a Xenopus Homolog of the Drosophila Barren Protein Tatsuya Hirano,

Isolation of a cRNP replicative intermediate and vRNP from influenza virus-infected cells. Isolation of a cRNP replicative intermediate and vRNP from influenza.

Purity of Lnp and its derivatives.

V-SVZ cultures express transcription factors expected for region of origin. V-SVZ cultures express transcription factors expected for region of origin.

Cryo-ET of stacks. Cryo-ET of stacks. (A) Human Lnp was reconstituted with phospholipids at a 1:5 molar ratio. The sample was analyzed by cryo-ET at −3.

Nucleosomes assembled with FACT have similar MNase digestion pattern as salt-reconstituted nucleosomes. Nucleosomes assembled with FACT have similar MNase.

Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7 cells or RPE cells. Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7.

DNA competes FACT away from H2A–H2B and facilitates formation of hexasomes/nucleosomes. DNA competes FACT away from H2A–H2B and facilitates formation of.

FACT does not disassemble nucleosomes assembled on α-satellite DNA.

Megakaryocyte total surface area increases with protrusion extension.

Identification of Z-FA-FMK as a potent compound that increases SMN protein expression in HEK293 and patient fibroblast cells. Identification of Z-FA-FMK.

Interference of Lnp domains with the formation of stacked bicelles.

FACT neither disassembles nor binds properly assembled nucleosomes.

Volume 111, Issue 3, Pages (November 2002)

The Zn2+-finger domain is not essential for the formation of stacked bicelles. The Zn2+-finger domain is not essential for the formation of stacked bicelles.

Reconstituted Xenopus Lnp forms stacked membrane discs.

Fibril-induced NM prions do not evolve from SGs.

Establishment of OL1 cells.

Volume 16, Issue 23, Pages (December 2006)

WW domain does not show dominant-negative effect on TBSV replication in a yeast cell extract. WW domain does not show dominant-negative effect on TBSV.

Kinesin 1 Drives Autolysosome Tubulation

Fig. 5 Analysis of VLPs formed from deletion CP mutants.

Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.

Mapping the Pirh2 and p73 interaction sites.

Volume 96, Issue 3, Pages (February 2009)

Western blot analysis of plasma proteins from two-dimensional gel electrophoresis. Western blot analysis of plasma proteins from two-dimensional gel electrophoresis.

Volume 113, Issue 2, Pages (July 2017)

DNA-binding and double-strand break formation by Vpr.

Volume 15, Issue 19, Pages (October 2005)

Volume 18, Issue 6, Pages (June 2010)

Fig. 5 Dcr-2 directly binds to Toll 3′UTR.

SDS-PAGE and Coomassie Blue Staining of Protein Samples after Various Purification Steps of Both Rice and Barley ADPG Hydrolytic NPPs.Lane numbering of.

Fig. 4 MxB intermolecular assembly interfaces and their role in the MxB assembly and HIV-1 inhibition. MxB intermolecular assembly interfaces and their.

Design of lipid-binding deficient mutants of α-synuclein.

Presentation transcript:

Lnp protein requires phospholipids to form stacked discs. Lnp protein requires phospholipids to form stacked discs. (A) Proteoliposomes containing full-length Xenopus Lnp were subjected to Nycodenz gradient centrifugation. Fractions (F1–F6) were collected from the top and analyzed by SDS-PAGE and Coomassie blue staining. (B) Non-reconstituted, purified full-length Xenopus Lnp was visualized by negative-stain EM at different concentrations. Scale bar, 20 nm. (C) Purified full-length Xenopus Lnp was reconstituted with phospholipids at a 1:5 protein-to-lipid ratio. Detergent was added after reconstitution and the sample was imaged by negative-stain EM. Scale bar, 20 nm. Songyu Wang et al. LSA 2018;1:e201700014 © 2018 Wang et al