Total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta are significantly correlated with fertilization rates after intracytoplasmic sperm injection Suseela Yelumalai, Ph.D., Marc Yeste, Ph.D., Celine Jones, Siti N. Amdani, M.Sc., Junaid Kashir, Ph.D., Ginny Mounce, M.Sc., Sarah J. Martins Da Silva, Ph.D., Christopher L. Barratt, Ph.D., Enda McVeigh, M.D., Kevin Coward, Ph.D. Fertility and Sterility Volume 104, Issue 3, Pages 561-568.e4 (September 2015) DOI: 10.1016/j.fertnstert.2015.05.018 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions
Figure 1 The proportion (%) of sperm exhibiting PLCζ (mean ± SEM) in controls and patients after IVF and ICSI treatment. Asterisk (∗) denotes significant (P<.05) differences between patients and controls. Fertility and Sterility 2015 104, 561-568.e4DOI: (10.1016/j.fertnstert.2015.05.018) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions
Figure 2 Mean fluorescent intensity (arbitrary units) of PLCζ levels per sperm (mean ± SEM) in controls and patients after IVF and ICSI treatment. Asterisk (∗) denotes significant (P<.05) differences between patients and controls. Fertility and Sterility 2015 104, 561-568.e4DOI: (10.1016/j.fertnstert.2015.05.018) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions
Supplemental Figure 1 Representative image of a human sperm showing equatorial and postacrosomal PLCζ immunostaining. BF = bright field; PA = PLCζ-staining in the postacrosomal region. Fertility and Sterility 2015 104, 561-568.e4DOI: (10.1016/j.fertnstert.2015.05.018) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions