Activating transcription factor 3 gene expression suggests that tissue stress plays a role in leiomyoma development Mark Payson, M.D., Minnie Malik,

Slides:

Advertisements

Similar presentations

Expression of the novel gene embryo implantation factor 2 (EMO2) in the mouse uterus at the implantation sites Zhaogui Sun, Ph.D., Renwei Su, Ph.D.,

Advertisements

Role of cAMP-responsive element modulator-τ (CREMτ) in ipsilateral testicular injury after unilateral testicular torsion-detorsion Si-Ming Wei, M.D.,

Tsai-Der Chuang, Ph.D., Omid Khorram, M.D., Ph.D.

Vascular endothelial growth factor A and its two receptors in human preantral follicles from fetuses, girls, and women Ronit Abir, Ph.D., Asangla Ao,

Shalmali J. Dharma, M. Sc. , Deepak N. Modi, Ph. D. , Tarala D

Association of abdominal obesity, insulin resistance, and oxidative stress in adipose tissue in women with polycystic ovary syndrome Li Chen, M.S., Wen.

Sandrine Giscard d’Estaing, Ph. D. , Delphine Perrin, M. Sc

Distinctive proliferative phase differences in gene expression in human myometrium and leiomyomata Hongbo Wang, MD, Mamatha Mahadevappa, PhD, Karen Yamamoto,

Down-regulation of HLA-G boosted natural killer cell-mediated cytolysis in JEG-3 cells cultured in vitro Li Li Sun, M.Sc., Yibing Han, Ph.D., Jian Hui.

Fertility and Sterility

Increased expression of platelet-derived growth factor C messenger ribonucleic acid in uterine leiomyomata Yuh-Ming Hwu, M.D., Sheng-Hsiang Li, Ph.D.,

Gene expression profiling of multiple leiomyomata uteri and matched normal tissue from a single patient Irina K. Dimitrova, M.D., Jennifer K. Richer,

Gene expression profiling of human peri-implantation endometria between natural and stimulated cycles Yunao Liu, M.Sc., Kai-Fai Lee, Ph.D., Ernest H.Y.

Expression of the insulin-like growth factor and insulin systems in the luteinizing macaque ovarian follicle Rebecca S. Brogan, Ph.D., Scott Mix, Muraly.

Curcumin, a nutritional supplement with antineoplastic activity, enhances leiomyoma cell apoptosis and decreases fibronectin expression Minnie Malik,

Tranilast, an orally active antiallergic compound, inhibits extracellular matrix production in human uterine leiomyoma and myometrial cells Md Soriful.

Infertile women with isolated polycystic ovaries are deficient in endometrial expression of osteopontin but not αvβ3 integrin during the implantation.

Chakradhari Sharan, Ph. D. , Sunil K. Halder, Ph. D

Progestin-induced heart and neural crest derivatives expressed transcript 2 is associated with fibulin-1 expression in human endometrial stromal cells

Altered expression of the tachykinins substance P/neurokinin A/hemokinin-1 and their preferred neurokinin 1/neurokinin 2 receptors in uterine leiomyomata

Expression of insulin-like growth factors (IGFs) and IGF signaling: molecular complexity in uterine leiomyomas Lan Peng, M.D., Yong Wen, M.D., Yulong.

Placental expression profiling in preeclampsia: local overproduction of hemoglobin may drive pathological changes Magnus Centlow, M.Sc., Piero Carninci,

Fas ligand+ fallopian tube epithelium induces apoptosis in both Fas receptor+ T lymphocytes and endometrial cells Sebastian E. Illanes, M.D., Kevin Maisey,

Expression of forkhead transcription factors in human granulosa cells

The antifibrotic drug halofuginone inhibits proliferation and collagen production by human leiomyoma and myometrial smooth muscle cells Meagan M. Grudzien,

Expression of cell cycle regulatory genes during primordial-primary follicle transition in the mouse ovary Chang-Eun Park, M.S., Kwang-Yul Cha, M.D.,

Presence of the brain proteins cerebral cavernous malformation-2 and cerebral cavernous malformation-3 in rat testes and their potential role in experimental.

Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization Yuri Mizusawa, M.D., Naoaki.

Endometrial stromal progesterone receptor-A/progesterone receptor-B ratio: no difference between women with and without endometriosis Davide Gentilini,

Dopamine receptor 2 activation inhibits ovarian vascular endothelial growth factor secretion in an ovarian hyperstimulation syndrome (OHSS) animal model:

Panagiotis Bakas, M. D. , Angelos Liapis, M. D

Endometrial expression and in vitro modulation of the iron transporter divalent metal transporter-1: implications for endometriosis Carlos Patricio Alvarado-Díaz,

Myometrial cells undergo fibrotic transformation under the influence of transforming growth factor β-3 Doina S. Joseph, M.S., Minnie Malik, Ph.D., Sahadat.

Comparison of two methods of in vivo gene transfer by electroporation

Sana M. Salih, M. D. , Salama A. Salama, Ph. D. , Amin A. Fadl, Ph. D

Age-associated alteration of oocyte-specific gene expression in polar bodies: potential markers of oocyte competence Ze-Xu Jiao, M.D., Ph.D., Min Xu,

Proteomic analysis of individual human embryos to identify novel biomarkers of development and viability Mandy G. Katz-Jaffe, Ph.D., David K. Gardner,

Nonobese diabetic/severe combined immunodeficient murine xenograft model for human uterine leiomyoma Guiwen Wang, M.D., Hiroshi Ishikawa, M.D., Ph.D.,

J. Browning Fitzgerald, B. S. , Vargheese Chennathukuzhi, Ph. D

Submucosal uterine leiomyomas have a global effect on molecular determinants of endometrial receptivity Beth W. Rackow, M.D., Hugh S. Taylor, M.D. Fertility.

Acetylated α-tubulin is reduced in individuals with poor sperm motility Shweta Bhagwat, M.Sc., Veena Dalvi, M.Sc., Darshan Chandrasekhar, M.Sc., Tinsu.

Expression of the novel gene embryo implantation factor 2 (EMO2) in the mouse uterus at the implantation sites Zhaogui Sun, Ph.D., Renwei Su, Ph.D.,

Expression of erythropoietin messenger ribonucleic acid in wild-type MED12 uterine leiomyomas under estrogenic influence: new insights into related growth.

Yan Wen, M. D. , Rudy Quintero, M. D. , Bertha Chen, M. D

Nonsense mutation of EMX2 is potential causative for uterus didelphysis: first molecular explanation for isolated incomplete müllerian fusion Shan Liu,

Monocyte chemotactic protein-1 (MCP-1), its receptor, and macrophages in the perifollicular stroma during the human ovulatory process Pernilla Dahm-Kähler,

Excessive ovarian response is associated with increased expression of interleukin-2 in the periimplantation endometrium Guneet Makkar, Ph.D., Ernest.

Increased progesterone receptor expression in uterine leiomyoma: correlation with age, number of leiomyomas, and clinical symptoms Anastasia Tsigkou,

Florian Schneider, M. D. , Klaus Redmann, Ph. D. , Joachim Wistuba, Ph

Aromatase expression in abdominal omental/visceral and subcutaneous fat depots: a comparison of pregnant and obese women Suman Rice, Ph.D., Bijal Patel,

Jieqiang Lv, M. D. , Xueqiong Zhu, M. D. , Ph. D

Overexpression of Fetuin-A Counteracts Ectopic Mineralization in a Mouse Model of Pseudoxanthoma Elasticum (Abcc6−/−) Qiujie Jiang, Florian Dibra, Michael.

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Müllerian-inhibiting substance inhibits cytochrome P450 aromatase activity in human granulosa lutein cell culture Michael P. Grossman, M.D., Steven T.

Development and validation of a three-dimensional in vitro model for uterine leiomyoma and patient-matched myometrium Minnie Malik, Ph.D., William H.

Epidermal growth factor–containing fibulin-like extracellular matrix protein 1 expression and regulation in uterine leiomyoma Erica E. Marsh, M.D., M.S.C.I.,

Human myometrium and leiomyomas express gonadotropin-releasing hormone 2 and gonadotropin-releasing hormone 2 receptor Jason D. Parker, D.O., Minnie.

Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D

Oral feeding and nasal instillation immunization with Microtus brandti lactate dehydrogenase c epitope DNA vaccine reduces fertility in mice via specific.

Expression of leucine-rich repeat-containing G-protein-coupled receptors in the human cyclic endometrium Claudia A. Krusche, Ph.D., Tina Kroll, Henning.

Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells Tadahiro Yasuo, M.D., Kotaro Kitaya, M.D. Fertility.

Human sperm cells express CD44

Daniela Zuccarello, Ph. D. , Andrea Garolla, Ph. D

Differences in gene expression in the proliferative human endometrium

William H. Catherino, M.D., Ph.D., Minnie Malik, Ph.D.

Nerea Subirán, J. D. , Ekaitz Agirregoitia, J. D. , Asier Valdivia, Ph

Jason Yen-Ping Ho, M. D. , Weng Chi Man, Ph. D. , Yan Wen, M. D

Tina Khanam, B.Sc., Geoffrey Burnstock, Ph.D., D.Sc.

Nicotine induces apoptosis in TM3 mouse Leydig cells

Kálmán A. Kovács, M. D. , Ph. D. , Ferenc Lengyel, Ph. D

Presentation transcript:

Activating transcription factor 3 gene expression suggests that tissue stress plays a role in leiomyoma development Mark Payson, M.D., Minnie Malik, Ph.D., Sarah Siti-nur Morris, James H. Segars, M.D., Rebecca Chason, M.D., William H. Catherino, M.D., Ph.D. Fertility and Sterility Volume 92, Issue 2, Pages 748-755 (August 2009) DOI: 10.1016/j.fertnstert.2008.06.030 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 ATF3 mRNA expression in leiomyoma and myometrium. Representative reverse-transcriptase PCR analyzing ATF3 levels in leiomyoma and myometrium samples (N = 7). Lane 1: LM, lane marker. Lane 2: negative control, ATF3 and 18S primers (no mRNA). Lane 3: 10 μg myometrial mRNA, ATF3 primers. Lane 4: 10 μg leiomyoma mRNA, ATF3 primers. Lane 5: 10 μg myometrial mRNA, ATF3, and 18S primers. Lane 6: 10 μg leiomyoma mRNA, ATF3, and 18S primers. Fertility and Sterility 2009 92, 748-755DOI: (10.1016/j.fertnstert.2008.06.030) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Assessment of ATF3 protein expression. (A) Western Blot showing ATF3 protein expression (21-kDa bands) using 10 μg each of representative myometrium (M) and leiomyoma (L) lysates (upper panel). β-Actin (42-kDa bands) was used as a control (lower panel). (B) Graphical representation of mean fold protein expression (leiomyoma:myometrium) for ATF3 protein in leiomyoma (black bar) and myometrium (white bar) from eight sample pairs determined by pixel intensity, P<.05 (two-tailed Student's t test). ATF3 was found to be more abundant in leiomyoma. (C) Immunohistochemical images of ATF3 protein in leiomyoma and corresponding myometrium at 63× magnification. Intracellular staining of ATF3 is demonstrated by the arrows. Similar staining patterns were seen in six different sample pairs (not shown). Fertility and Sterility 2009 92, 748-755DOI: (10.1016/j.fertnstert.2008.06.030) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) mRNA expression of AP-1 transcripts. Graphical representation of mean fold mRNA expression (leiomyoma:myometrium) for cJun and cFos, respectively, in leiomyoma (black bars) and myometrium (white bars) from 10 sample pairs. Expression values were determined by real-time reverse transcriptase PCR using 10 ng of mRNA isolated from leiomyoma and myometrium samples, respectively. Significance was determined using a two-tailed t test. The mean fold mRNA expression for Jun was found to be −29.56, SEM of 14.9 (P<.05). Fos was found to be −37.97 with an SEM of 15.1 (P<.05). (B) Protein expression cJun and cFos. Graphical representation of mean fold protein expression (leiomyoma:myometrium) for cJun and cFos, respectively, in leiomyoma (black bars) and myometrium (white bars) from eight sample pairs. Values were determined by pixel intensity from Western blot experiments. The mean fold protein expression for cJun and cFos were found to be 1.2 and 1.3, respectively. Protein levels between myometrium and leiomyoma did not differ significantly using a two-tailed t test. Fertility and Sterility 2009 92, 748-755DOI: (10.1016/j.fertnstert.2008.06.030) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions