Claudia Bohring, Ph.D., Jan Skrzypek, M.D., Walter Krause, M.D.

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Presentation transcript:

Influence of antisperm antibodies on the acrosome reaction as determined by flow cytometry Claudia Bohring, Ph.D., Jan Skrzypek, M.D., Walter Krause, M.D. Fertility and Sterility Volume 76, Issue 2, Pages 275-280 (August 2001) DOI: 10.1016/S0015-0282(01)01924-0

Figure 1 Typical distribution after staining spermatozoa with propidium iodide (FL3) and FITC-labeled antibody (FL1), as obtained by flow cytometry. Each dot represents one spermatozoa. (A), The spontaneous acrosome reaction (SAR) of spermatozoa. (B), Spermatozoa after AR induced by calcium ionophore (IAR), in which a shift of the number of FITC-stained spermatozoa, corresponding to the binding of TÜS-19 to AR spermatozoa, is obvious. 1 = vital spermatozoa, acrosome reacted; 2 = vital spermatozoa, acrosome unreacted; 3 = avital spermatozoa, acrosome reacted; 4 = avital spermatozoa, acrosome unreacted. Bohring. Antisperm antibodies and acrosome reaction. Fertil Steril 2001. Fertility and Sterility 2001 76, 275-280DOI: (10.1016/S0015-0282(01)01924-0)

Figure 2 Comparison between the two control groups: C1, control group without seminal plasma; and C2, control group with seminal plasma free of antisperm antibodies. The admixture of seminal plasma samples without antisperm antibodies showed no significant response in the spontaneous acrosome reaction (SAR) or the induced acrosome reaction (IAR), or the acrosome response to ionophore challenge (ARIC), which was induced in donor spermatozoa. The columns represent the median of each group. The standard derivation is marked. Bohring. Antisperm antibodies and acrosome reaction. Fertil Steril 2001. Fertility and Sterility 2001 76, 275-280DOI: (10.1016/S0015-0282(01)01924-0)

Figure 3 Comparison between groups C2, the control group of seminal plasma without antisperm antibodies, and T, seminal plasma containing antisperm antibodies. The admixture of seminal plasma samples with antisperm antibodies showed a significant increase in the spontaneous acrosome reaction (SAR), induced acrosome (IAR), and the acrosome response to ionophore challenge (ARIC), which was triggered in donor spermatozoa. The columns represent the median of each group. The standard derivation is marked. Bohring. Antisperm antibodies and acrosome reaction. Fertil Steril 2001. Fertility and Sterility 2001 76, 275-280DOI: (10.1016/S0015-0282(01)01924-0)