Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment Csaba Pribenszky, Eszter Losonczi,

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Presentation transcript:

Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment Csaba Pribenszky, Eszter Losonczi, Miklós Molnár, Zsolt Lang, Szabolcs Mátyás, Klára Rajczy, Katalin Molnár, Péter Kovács, Péter Nagy, Jason Conceicao, Gábor Vajta Reproductive BioMedicine Online Volume 20, Issue 3, Pages 371-379 (March 2010) DOI: 10.1016/j.rbmo.2009.12.007 Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Embryos in the microwells in the bottom of a 35-mm Petri dish as presented online by the controlling PC; (A) 2- to 4-cell-stage embryos on day 2; (B) embryo development on day 5; and (C) embryo development on day 6. Reproductive BioMedicine Online 2010 20, 371-379DOI: (10.1016/j.rbmo.2009.12.007) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Compact, digital inverted microscopes inside the incubator. The well-of-the-well dishes are positioned under the lamp console. Reproductive BioMedicine Online 2010 20, 371-379DOI: (10.1016/j.rbmo.2009.12.007) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Observing embryo development online on the screen of the PC. Reproductive BioMedicine Online 2010 20, 371-379DOI: (10.1016/j.rbmo.2009.12.007) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 Probability of a non-fragmented embryo developing to the blastocyst stage in relation to the time of the first and second cleavages. The cleavage data of 90 embryos are presented on the image. Each data point (circles) represents an embryo with the corresponding cleavage times. Filled circles=embryo reached the blastocyst stage; open circles=embryo did not reach the blastocyst stage. Reproductive BioMedicine Online 2010 20, 371-379DOI: (10.1016/j.rbmo.2009.12.007) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 5 Probability of a fragmented embryo developing to the blastocyst stage in relation to the time of the first and second cleavages. The cleavage data of 11 embryos are presented on the image. Each data point (circles) represents an embryo with the corresponding cleavage times. Filled circles=embryo reached the blastocyst stage; open circles=embryo did not reach the blastocyst stage. Reproductive BioMedicine Online 2010 20, 371-379DOI: (10.1016/j.rbmo.2009.12.007) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 6 Duration of the appearing/disappearing fragments. The average time for fragments to appear/disappear was 546±442min (dotted line). Reproductive BioMedicine Online 2010 20, 371-379DOI: (10.1016/j.rbmo.2009.12.007) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 7 Reduction of the probability of reaching the blastocyst stage if a fragmentation occurs during embryo development. Data for 11 fragmented embryos were analysed. If the first and second cleavage of an embryo occurred before 1300 and 1200min, respectively, the chance of blastocyst development (∼100%) is independent of the occurrence of fragmentation. If the first and second cleavage of an embryo occurred after 1500 and 1700min, respectively, the chance of blastocyst development (∼0%) is also independent from the occurrence of fragmentation. However, if the first and second cleavage of an embryo occurred between these time-intervals, respectively, a fragmented embryo may have a 60% less chance to develop to blastocyst than a non-fragmented embryo. Reproductive BioMedicine Online 2010 20, 371-379DOI: (10.1016/j.rbmo.2009.12.007) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions