A New Noncanonical Nuclear Genetic Code

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A New Noncanonical Nuclear Genetic Code Rocı́o Sánchez-Silva, Eduardo Villalobo, Loı̈c Morin, Antonio Torres  Current Biology  Volume 13, Issue 5, Pages 442-447 (March 2003) DOI: 10.1016/S0960-9822(03)00126-X

Figure 1 Alignment of Deduced Amino Acid Sequences of Actin Vorticella microstoma, Opisthonecta heneguyii, and Opisthonecta matiensis actin are aligned to actin from rabbit (accession number NM_007392), Arabidopsis thaliana (accession number M20016), Chlamydomonas reinhardtii (accession number D50838.1), Tetrahymena thermophila (accession number M13939), and Paramecium tetraurelia (our unpublished data). Filled circles and filled triangles denote UAA and UAG codons, respectively, at the nucleotide sequence (see Figure S1). Gaps, introduced to improve alignments, are indicated by dots. The black and gray shadowed amino acids represent, respectively, glutamate and glutamine residues encoded by UAR in ciliates. The numbers on top of the alignments refer to the sequence in the first row. Rab, rabbit; Atha, Arabidopsis thaliana; Cre, Chlamydomonas reinhardtii; Tth, Tetrahymena thermophila; Pte, Paramecium tetraurelia; Vmi, Vorticella microstoma; Ohe, Opisthonecta henneguyi; Oma, Opisthonecta matiensis. Current Biology 2003 13, 442-447DOI: (10.1016/S0960-9822(03)00126-X)

Figure 2 Alignment of Deduced Amino Acid Sequences of α-Tubulin V. microstoma, Opisthonecta heneguyii, and Opisthonecta matiensis α-tubulin sequences are aligned to α-tubulin from Mus musculus (accession number BC008117.1), Arabidopsis thaliana (accession number AY091372.1), Chlamydomonas reinhardtii (accession number M11447.1), Tetrahymena thermophila (accession number M86723), and Paramecium tetraurelia (accession number X99489). Filled circles and filled triangles denote UAA and UAG codons, respetively, at the nucleotide sequence (see Figure S1). The black and gray shadowed amino acids represent, respectively, glutamate and glutamine residues encoded by UAR in ciliates. The numbers on top of the alignments refer to the sequence in the first row. Mmus, Mus musculus; Atha, Arabidopsis thaliana; Cre, Chlamydomonas reinhardtii; Tth, Tetrahymena thermophila; Pte, Paramecium tetraurelia; Vmi, Vorticella microstoma; Ohe, Opisthonecta henneguyi; Oma, Opisthonecta matiensis. Current Biology 2003 13, 442-447DOI: (10.1016/S0960-9822(03)00126-X)

Figure 3 Consensus Tree Based on α-Tubulin-Deduced Amino Acid Sequences The resulting bootstrap values are displayed at each node; neighbor-joining, parsimony, and maximum likelihood are listed from left to right. The scale bar represents 0.6083 expected amino acid replacements per position. Current Biology 2003 13, 442-447DOI: (10.1016/S0960-9822(03)00126-X)