Volume 120, Issue 6, Pages (May 2001)

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Volume 120, Issue 6, Pages 1412-1419 (May 2001) Expression of the duodenal iron transporters divalent-metal transporter 1 and ferroportin 1 in iron deficiency and iron overload Heinz Zoller, Günter Weiss, Igor Theurl, Robert O. Koch, Wolfgang Vogel Gastroenterology Volume 120, Issue 6, Pages 1412-1419 (May 2001) DOI: 10.1053/gast.2001.24033 Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 1 Quantification of duodenal DMT1 cDNA expression by real-time PCR. Results of TaqMan real-time PCR of 5 representative patients are shown. Fluorescence activity (ΔRn) is blotted vs. cycle number. (●) Normal patient; (▿) iron-deficient patient; (♦) C282Y patient; (○) non-HFE HH patient; (×) patient with secondary iron overload. For details, see Materials and Methods. Gastroenterology 2001 120, 1412-1419DOI: (10.1053/gast.2001.24033) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 2 DMT1 and FP1 cDNA levels in duodenal biopsy specimens from patients with disorders of iron metabolism. (A) DMT1/GAPDH cDNA ratio in logarithmic scale, median (horizontal line), 75% confidence interval (box), and minimal and maximal values (vertical line) in the respective patient groups. (B) Logarithmic FP1/GAPDH cDNA ratio (median, 75% confidence interval, and minimal and maximal values). Gastroenterology 2001 120, 1412-1419DOI: (10.1053/gast.2001.24033) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 3 DMT1 protein expression in duodenal biopsy specimen Western blot analysis for DMT1 of protein extracts from duodenal biopsy specimens. A representative example of each patient group is shown. The 60-kilodalton band corresponds to the DMT1 protein. Relative intensities were estimated from densitometric scanning of Western membranes and are expressed as percentages of control (100%; means ± SD for 4 experiments). *P < 0.05 compared with control. Gastroenterology 2001 120, 1412-1419DOI: (10.1053/gast.2001.24033) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 4 Distribution of DMT1 protein in patients with various iron disorders. Immunohistochemical staining for DMT1 of duodenal biopsy specimen from 5 representative patients is shown. Staining is most prominent on the apical membrane of duodenal enterocytes. (A) Patient with normal serum iron parameters; (B) iron-deficient patient; (C) HFE-HH (C282Y homozygous); (D) non-HFE HH; (E) secondary iron overload. Gastroenterology 2001 120, 1412-1419DOI: (10.1053/gast.2001.24033) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 5 Distribution of FP1 in biopsy specimens from normal, iron-deficient, and iron-overloaded patients. Representative cryosections of duodenal biopsy specimens from (A) a normal patient, (B) an iron-deficient patient, and (C) a patient with HFE HH are shown. The staining is more prominent in anemic and iron-overloaded patients than in the control patient, and the highest intensity is in the basolateral membrane. Gastroenterology 2001 120, 1412-1419DOI: (10.1053/gast.2001.24033) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 6 Association between duodenal DMT1 and FP1 mRNA expression. Correlation between DMT1/GAPDH and FP1/GAPDH ratios is shown in all patients investigated independent of underlying disease (all single values; the regression line and 95% confidence intervals are plotted). Gastroenterology 2001 120, 1412-1419DOI: (10.1053/gast.2001.24033) Copyright © 2001 American Gastroenterological Association Terms and Conditions