Volume 8, Issue 9, Pages 1431-1433 (September 2015) DSDecode: A Web-Based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations  Weizhi Liu, Xianrong Xie, Xingliang Ma, Jun Li, Jiehu Chen, Yao-Guang Liu  Molecular Plant  Volume 8, Issue 9, Pages 1431-1433 (September 2015) DOI: 10.1016/j.molp.2015.05.009 Copyright © 2015 The Author Terms and Conditions

Figure 1 The Workflow of DSDecode/SaDSDecode and the Resulting Output. (A) The main steps of the DSDecode/SaDSDecode workflow. AS, anchor sequence; DS, degenerate sequence; DB, deleted bases; L-DS, long DS. If a large deletion equal to or longer than the primary DS is detected in an allele, a new L-DS is reset by the program for decoding again. If automatic decoding fails, semi-automatic decoding (SaDSDecode) can be used. (B and C) Data input areas of DSDecode and the resulting display. Additional “-” indicates a base deletion in the decoded allele(s) or the corresponding position of a base insertion in the reference sequence. In most cases, it is not necessary to change the default settings of 20 nt for AS and 15 nt for DS. (D and E) Data input areas of SaDSDecode and the resulting display. This case was derived from a chromatogram shown in Supplemental Figure 2C (3). Molecular Plant 2015 8, 1431-1433DOI: (10.1016/j.molp.2015.05.009) Copyright © 2015 The Author Terms and Conditions