Protein microarrays for validation of antibodies.

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Presentation transcript:

Protein microarrays for validation of antibodies. Protein microarrays for validation of antibodies. The results of the specificity analysis of two pairs of monospecific antibodies on protein arrays with 1440 PrESTs are shown. Each pair (a and b and c and d) corresponds to antibodies generated against the same antigen in two different animals. The red bar corresponds to the relative signal from the specific antigen, and the black bars represent the cross-reactivity to the other PrESTs. In each graph is an image from one-fourth of the array inserted. A dual color system is used where the presence of all proteins is detected in green, and the specific antibody is detected in red. a and b, antibodies HPR000534 and HPR001102 directed against a DnaJ homolog protein. c and d, antibodies HPR000830 and HPR001088 directed against a predicted KIAA protein. Mathias Uhlén et al. Mol Cell Proteomics 2005;4:1920-1932 The American Society for Biochemistry and Molecular Biology