Cardiomyocyte Cell-Cycle Activity during Preadolescence

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Cardiomyocyte Cell-Cycle Activity during Preadolescence Mark H. Soonpaa, David C. Zebrowski, Colin Platt, Anthony Rosenzweig, Felix B. Engel, Loren J. Field Cell Volume 163, Issue 4, Pages 781-782 (November 2015) DOI: 10.1016/j.cell.2015.10.037 Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 1 Characterization of Cardiomyocyte Cell-Cycle Parameters during Preadolesence (A) Example of cardiomyocyte DNA synthesis (arrow) in the heart of an MHC-nLAC mouse carrying a BrdU mini-pump from PN10 through PN19. Panels show Hoechst staining of DNA (blue signal), beta-galactosidase immune reactivity (red signal) and BrdU immune reactivity (green signal). Scale bar, 10 microns. (B) BrdU immune reactivity (green signal) in the small intestine of an MHC-nLAC mouse carrying a BrdU mini-pump for 24 hr (left) or 9 days (right). Arrows, villi crypts. Scale bar, 200 microns. (C) Example of cardiomyocyte DNA synthesis (arrow) in dispersed cells from a C57Bl/6J mouse heart carrying a BrdU mini-pump from PN10 through PN19. Panels show Hoechst staining of DNA (blue signal) and BrdU immune reactivity (green signal). Scale bar, 20 microns. (D) Example of an S-phase cardiomyocyte nucleus in a PN15 heart as evidenced by co-localization of Nkx2.5 (red signal) and Ki-67 (green signal) immune reactivity (arrow). Scale bar, 10 microns. (E) Example of cardiomyocyte (upper panel) and non-cardiomyocyte (lower panel) H3P immune reactivity (green signal, arrow) in postnatal hearts. Cardiomyocytes were identified by α-actinin immune reactivity (red signal). Scale bar, 10 microns. (F) Cardiac expression of mitosis-related genes on PN10, PN14, PN15, PN16, and PN21 in C57BL/6J mice (mean ± SEM) relative to their level at P10. mRNA levels were quantitated and normalized to 18S as described (Livak and Schmittgen, 2001). Significance was tested using unpaired, 2-tailed Student’s t tests with Bonferroni correction for multiple testing; bars indicate p < 0.05 versus subsequent time point. Cell 2015 163, 781-782DOI: (10.1016/j.cell.2015.10.037) Copyright © 2015 Elsevier Inc. Terms and Conditions