The Thermodynamic Meaning of Metabolic Exchange Fluxes

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The Thermodynamic Meaning of Metabolic Exchange Fluxes Wolfgang Wiechert Biophysical Journal Volume 93, Issue 6, Pages 2255-2264 (September 2007) DOI: 10.1529/biophysj.106.099895 Copyright © 2007 The Biophysical Society Terms and Conditions

Figure 1 Reaction mechanism examples of increasing complexity used to demonstrate the application of the three computational rules for apparent forward/backward flux quotients: (a) General bidirectional multistep Michaelis-Menten mechanism for a unimolecular reaction S→P. (b) Sequential mechanism for a unimolecular reaction S→P with two cofactors T, Q not taken into account for isotopic labeling. (c) Sequential binding mechanism for a bimolecular reaction S+T→P. (d) Random binding mechanism for a bimolecular reaction S+T→P. (e) Random bi-bi reaction mechanism with multiple substrates and products S+T→P+Q. S, T are substrates, P, Q are products, E is free enzyme, and Mi, ES, ET, EST, and ESPQ are enzyme complexes. Shaded circles indicate the potentially labeled substrates and products. Biophysical Journal 2007 93, 2255-2264DOI: (10.1529/biophysj.106.099895) Copyright © 2007 The Biophysical Society Terms and Conditions

Figure 2 Isotope-labeling networks for the reaction mechanism from Fig. 1 d. (a) Tracing an S atom to P. (b) Tracing a T atom to P. Dashed arrows indicate substances which enter or leave the network but are not considered for isotope tracing. Biophysical Journal 2007 93, 2255-2264DOI: (10.1529/biophysj.106.099895) Copyright © 2007 The Biophysical Society Terms and Conditions